Composition of mitochondrial DNA COI nucleotide of striped snakehead (Channa striata bloch, 1793) collected from three rivers in Sumatra and Kalimantan

Arisuryanti, Tuty; Vicken,; ꞌAzizah, Nur; Ratman, Nofita; Julaeha, Ade Siti; Firdaus, Nadya Ulfa Nida’; Hakim, Lukman

doi:10.1063/5.0015907

Cited by 1 publication

(1 citation statement)

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“…The CG content values not exceeding 50% were also detected in 6 species from 2 genera consisted of Ompok pabda (49.3%), Ompok pabo (46.4%), and Ompok bimaculatus (45.6%) (Malakar et al 2012), Kryptopterus apogon (46.40%), Kryptopterus micronema (46.40%), and Kryptopterus limpok (46.40%) (Jusmaldi et al 2017). This total can also be found for several marine fish species (Xu et al 2021) and freshwater fish species (Arisuryanti et al 2020b;Pandey et al 2020). All samples showed identical sequences (only one haplotype) indicating no sequence variation within a population.…”

Section: Resultsmentioning

confidence: 63%

Coi-Based Dna Barcoding of Selais Fish From Arut River, Central Kalimantan, Indonesia

Kasayev

Arisuryanti

2022

J.Tropical Biodiversity Biotechnology

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Selais fish belongs to famili Siluridae consisting of 12 genera with 104 properly validated species. The human need for these fish has sharply increased due to the benefits provided especially for consumption. However, morphologically the selais fish are slightly difficult to differentiate among other silurid fish for non-specialist experts. Thus, a DNA barcoding approach using the mitochondrial COI gene as a molecular marker in this study was applied to clarify a taxonomic status and classification species of selais fish from Arut River (Central Kalimantan, Indonesia) and was also to assembly COI database library of the fish from Indonesia. The method used in this study was a PCR method with a pair of universal primers FishF2 and FishR2. Based on COI-based DNA barcoding, the whole samples showed there were no sequence differences (only 1 haplotype) within the population and this confirmed that these fish only consisted of one identical species. Furthermore, phylogenetic analysis (NJ / ML / BI) revealed that selais fish in this study had a close genetic relationship with Ompok hypophthalmus compared to other Ompok groups. This relationship was supported by the genetic distance value not exceeding 3.6% and this evaluated the undetermined naming of the selais fish from Arut River which was previously still unclassifiable.

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