Composition, diversity and bioactivity of culturable bacterial endophytes in mountain-cultivated ginseng in Korea

Chowdhury, Md. Emran Khan; Jeon, Junhyun; Rim, Soon Ok; Park, Young-Hwan; Lee, Seung Kyu; Bae, Hyeun‐Jong

doi:10.1038/s41598-017-10280-7

Cited by 58 publications

(49 citation statements)

References 44 publications

(47 reference statements)

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“…Recently, the indole-3-carboxylic acid (IAA) and other auxins have been shown to stimulate cell elongation, resulting in root growth initiation or an enhancement of nutritional elements absorption by the hosts [104,105]. Besides, IAA was supposed to improve the adaptability of plant microbe interaction [106]. are shown next to the branches.…”

Section: Discussionmentioning

confidence: 99%

Diversity and bioactivities of fungal endophytes from Distylium chinense, a rare waterlogging tolerant plant endemic to the Three Gorges Reservoir

Duan

Qin

et al. 2019

Preprint

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Background The present study focuses on diversity and biological activities of the endophytic fungal community from Distylium chinense , a rare waterlogging tolerant plant endemic to the Three Gorges Reservoir. This study has been explored the characteristics of endophytic fungi in waterlogged environment including both aquatic and terrestrial fungi, and they may produce new metabolites under complex and extreme conditions, which may possess bioactive property. Therefore, the antioxidant, antimicrobial and anticancer activities of all endophytes isolated from this study have been investigated. Moreover, the active metabolites of the most broad-spectrum bioactive strain have also been studied.Results A total of 154 fungal endophytes were isolated from roots and stems. They were categorized into 30 morphotypes based on cultural characteristics and were affiliated with 27 different taxa. Among these, the most abundant fungal orders included Diaporthales (34.4%) and Botryosphaeriales (30.5%), which were predominantly represented by the species Phomopsis sp. (24.7%) and Neofusicoccum parvum (23.4%). Fermentation extracts were evaluated, screening for antioxidant, antimicrobial and anticancer activities. Among the 154 isolates tested, 99 (64.3%) displayed significant antioxidant activity, 153 (99.4%) exhibited inclusive antimicrobial activity against at least one tested microorganism and 27 (17.5%) showed exclusive anticancer activity against one or more cancer cell lines. Specifically, the crude extract of Irpex lacteus DR10-1 exhibited note-worthy bioactivities. Further chemical investigation on DR10-1 strain resulted in the isolation and identification of two known bioactive metabolites, indole-3-carboxylic acid ( 1 ) and indole-3-carboxaldehyde ( 2 ), indicating their potential roles in plant growth promotion and human medicinal value.Conclusions These results indicated that diverse endophytic fungal population inhabits D. chinense . The isolated endophyte DR10-1 ( Irpex lacteus ) has the potential to be a source of novel antioxidant/antimicrobial/anticancer compounds. The findings of the present study not only provide a sustainable resource for the utilization of endophytic fungi in D. chinense but also provides an important basis for further understanding of fungal communities in medicinal plants.

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Section: Discussionmentioning

confidence: 99%

Diversity and bioactivities of fungal endophytes from Distylium chinense, a rare waterlogging tolerant plant endemic to the Three Gorges Reservoir

Duan

Qin

et al. 2019

Preprint

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“…The transport of ginsenosides from various tissue parts needs to be explored in future experiments. Moreover, ginseng is typically grown in a farm setting under artificial shade conditions often involving pesticides and fertilizers [20,21], and managed by unified practice. Whereas this ginseng is certified organic grown under the shade of a pine forest, accumulation of ginsenosides can be affected by some uncontrolled factors, including soil, moisture, and light [22].…”

Section: Quantification Of the Main Ginsenosides In Various Parts Of mentioning

confidence: 99%

Comparison of Ginsenoside Components of Various Tissues of New Zealand Forest-Grown Asian Ginseng (Panax Ginseng) and American Ginseng (Panax Quinquefolium L.)

2020

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Asian ginseng (Panax ginseng) and American ginseng (Panax quinquefolium L.) are the two most important ginseng species for their medicinal properties. Ginseng is not only popular to consume, but is also increasingly popular to cultivate. In the North Island of New Zealand, Asian ginseng and American ginseng have been grown in Taupo and Rotorua for more than 15 years. There are no publications comparing the chemical constituents between New Zealand-grown Asian ginseng (NZPG) and New Zealand-grown American ginseng (NZPQ). In this study, fourteen ginsenoside reference standards and LC–MS2 technology were employed to analyze the ginsenoside components of various parts (fine root, rhizome, main root, stem, and leaf) from NZPG and NZPQ. Fifty and 43 ginsenosides were identified from various parts of NZPG and NZPQ, respectively, and 29 ginsenosides were found in both ginseng species. Ginsenoside concentrations in different parts of ginsengs were varied. Compared to other tissues, the fine roots contained the most abundant ginsenosides, not only in NZPG (142.49 ± 1.14 mg/g) but also in NZPQ (115.69 ± 3.51 mg/g). For the individual ginsenosides of both NZPG and NZPQ, concentration of Rb1 was highest in the underground parts (fine root, rhizome, and main root), and ginsenoside Re was highest in the aboveground parts (stem and leaf).

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“…The extracted genomic DNA was prepared and used as a template. 1.5 kb 16S rRNA genes were amplified using bacterial universal primers (Biomarker biotechnology, Beijing, China) 27F (5 -AGAGTTTGATCCTGGCTCAG-3 ) and 1492R (5 -GGTTACC TTGTTACGA CTT-3 ) [19]. The PCR mixture included 0.5 µM of each primer, 23.5 µL of nuclease-free water, 25 µL master mix, and 1 µL of DNA template, making up a total of 50 µL.…”

Section: Extraction Of Dna and Pcr Amplificationmentioning

confidence: 99%

“…October in the Nanping region. The samples from this region had the highest number of phyla (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26).…”

Section: Composition Of the Structural Diversity Of Bacteriamentioning

confidence: 99%

Seasonal Diversity of Endophytic Bacteria Associated with Cinnamomum camphora (L.) Presl.

2019

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Investigations on the density, diversity, and distribution of endophytic bacterial community associated with leaves of Cinnamomum camphora (L.) Presl. were carried out during three seasons using 16s rDNA high-throughput sequencing technology. Samples were collected from five species in Nanping (A, B, C, D, and E) and one from Fuqin (F) in the Fujian province, China in the months of April, July, and October (represented by 1, 2 and 3), indicating spring, summer, and early winter, respectively. Results from 16s rDNA sequences revealed 10,844,124 effective sequences. The highest OTUs (Operational taxonomic units) was highest in the A1 sample (1086), while the lowest was in C2 (509). Our observations showed that samples taken in October had the highest diversity of endophytes as indicated by the Shannon index (B3 = 5.3337), Chao1 (E3 = 1233.10), abundance-based coverage estimator (ACE) (A3 = 1764.72), and the Simpson indices of diversity (C3 = 0.1655) irrespective of the species. The order of the endophytes richness in the samples was April > July > October. The Ribosomal Database Project (RDP) classifier showed that the obtained sequences belonged to nine major phyla: Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Gemmatimonadetes, Acidobacteria, Planctomycetes, Chloroflexi, and Fusobacteria. Proteobacteria accounted for the highest proportion in each sample, ranging from 35.15% to 89.72%. These sequences belonged mainly to 10 orders: Rhizobiales, Clostridiales, Peseudomonadales, Burkholderiales, Bacteroidales, Enterobacteriales, Rhodocyclales, Sphingomonadales, Lactobacillales, and Bacillales. Also, other taxa with possible taxonomic statuses, which were unclassified, were present.

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Composition, diversity and bioactivity of culturable bacterial endophytes in mountain-cultivated ginseng in Korea

Cited by 58 publications

References 44 publications

Diversity and bioactivities of fungal endophytes from Distylium chinense, a rare waterlogging tolerant plant endemic to the Three Gorges Reservoir

Diversity and bioactivities of fungal endophytes from Distylium chinense, a rare waterlogging tolerant plant endemic to the Three Gorges Reservoir

Comparison of Ginsenoside Components of Various Tissues of New Zealand Forest-Grown Asian Ginseng (Panax Ginseng) and American Ginseng (Panax Quinquefolium L.)

Seasonal Diversity of Endophytic Bacteria Associated with Cinnamomum camphora (L.) Presl.

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