Composition and variability of phospholipids in Chinese human milk samples

Li, Siming; Chen, Yujie; Han, Bin; Xu, Tao; Liu, Tongjie; Yi, Huaxi; Zhou, Xin; Zhang, Lanwei; Liu, Ping; Ma, Cheng; Li, Yuanyuan; Pan, Jiancun; Jiang, Shilong

doi:10.1016/j.idairyj.2020.104782

Cited by 11 publications

(2 citation statements)

References 35 publications

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“…Mature bovine milk was collected during the mid-lactation period and used in most experiments unless stated otherwise. Human milk samples were obtained as previously described ( 62 ). Briefly, fresh human milk samples were collected by means of an electric breast pump from women aged 25 to 35 years who were at a lactational stage of 15 to 180 days.…”

Section: Methodsmentioning

confidence: 99%

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis

et al. 2023

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Milk-derived extracellular vesicles (mEVs) have been proposed as a potential nanomedicine for intestinal disorders; however, their impact on intestinal barrier integrity in gut inflammation and associated metabolic diseases has not been explored yet. Here, mEVs derived from bovine and human breast milk exert similar protective effects on epithelial tight junction functionality in vitro, survive harsh gastrointestinal conditions ex vivo, and reach the colon in vivo. Oral administration of mEVs restores gut barrier integrity at multiple levels, including mucus, epithelial, and immune barriers, and prevents endotoxin translocation into the liver in chemical-induced experimental colitis and diet-induced nonalcoholic steatohepatitis (NASH), thereby alleviating gut disorders, their associated liver inflammation, and NASH. Oral administration of mEVs has potential in the treatment of gut inflammation and gut-liver axis–associated metabolic diseases via protection of intestinal barrier integrity.

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“…However, due to the lack of electroactive chromophores and fluorescent groups in the choline molecule, , the techniques that can be applied to the detection are very limited. The universal detection methods mainly include chromatography, − electrochemical methods, ,− and chemiluminescence methods. − Despite the high accuracy, there are still some unavoidable limitations, such as complex sample preparation, long separation time, substantial consumption of organic reagents, complicated electrode modification, and reliance on large-scale equipment . Compared with traditional methods, enzyme biosensors have attracted wide attention in recent years for their simple, rapid, sensitive, and efficient advantages. , Some of them are mediated by choline oxidase (ChO x ), which can oxidize choline into betaine and hydrogen peroxide (H 2 O 2 ), and the choline concentration would be further calculated by the generated H 2 O 2 . − However, the detection process requires a series of standard configuration, sample determination, and other steps; particularly, multi-step operation is very complex under different temperatures and pH values. − Meanwhile, the utilized oxidoreductase exhibits high costs, complex purification, and poor stability.…”

Section: Introductionmentioning

confidence: 99%

Choline Oxidase-Integrated Copper Metal–Organic Frameworks as Cascade Nanozymes for One-Step Colorimetric Choline Detection

Huang

Song

Zhang

et al. 2022

J. Agric. Food Chem.

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Choline is an important factor for regulating human health and is widely present in various foods. In this work, a sensor strategy based on a choline oxidase-integrated copper(II) metal−organic framework with peroxidase-like activity is constructed for one-step cascade detection of choline. The one-step cascade strategy can avoid intermediate product transferring in general multi-step reactions, and the multi-enzyme activities can be well exerted under one condition, thus exhibiting excellent catalytic activity and enhanced stability. In the integrated system, choline is catalyzed by ChO x to produce betaine and H 2 O 2 , which eventually got converted to hydroxyl radicals by the peroxidase nanozyme, oxidized the chromogenic substrate ABTS, and produced an observable absorption peak at 420 nm. A new choline detection method was thus established and showed a satisfactory linear relationship at 6−300 μM, which has been used for the choline analysis in milk.

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Composition and variability of phospholipids in Chinese human milk samples

Cited by 11 publications

References 35 publications

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis

Choline Oxidase-Integrated Copper Metal–Organic Frameworks as Cascade Nanozymes for One-Step Colorimetric Choline Detection

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