Composite Scaffolds for Bone Tissue Regeneration

Navarro, Melba; Planell, Josep A.

doi:10.1002/9781118097298.weoc050

Cited by 4 publications

(6 citation statements)

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“…There is an extensive list of polymers that have been assessed for use in bone tissue replacement, both of natural and synthetic origin which have been extensively reviewed elsewhere [ 6 , 131 , 132 , 133 , 134 ]. Natural polymers such as chitosan, alginate and hyaluronic acid as well as proteins such as collagen, fibrin and silk show promise due to their inherent biocompatibilities and biodegradabilities [ 135 ]. Many of these polymers also contain protonable functional groups which can be easily utilized in order to deliver bioactive factors such as proteins, drugs and nucleic acids [ 136 , 137 , 138 ].…”

Section: Tissue Engineeringmentioning

confidence: 99%

Chitosan for Gene Delivery and Orthopedic Tissue Engineering Applications

2013

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Gene therapy involves the introduction of foreign genetic material into cells in order exert a therapeutic effect. The application of gene therapy to the field of orthopaedic tissue engineering is extremely promising as the controlled release of therapeutic proteins such as bone morphogenetic proteins have been shown to stimulate bone repair. However, there are a number of drawbacks associated with viral and synthetic non-viral gene delivery approaches. One natural polymer which has generated interest as a gene delivery vector is chitosan. Chitosan is biodegradable, biocompatible and non-toxic. Much of the appeal of chitosan is due to the presence of primary amine groups in its repeating units which become protonated in acidic conditions. This property makes it a promising candidate for non-viral gene delivery. Chitosan-based vectors have been shown to transfect a number of cell types including human embryonic kidney cells (HEK293) and human cervical cancer cells (HeLa). Aside from its use in gene delivery, chitosan possesses a range of properties that show promise in tissue engineering applications; it is biodegradable, biocompatible, has anti-bacterial activity, and, its cationic nature allows for electrostatic interaction with glycosaminoglycans and other proteoglycans. It can be used to make nano- and microparticles, sponges, gels, membranes and porous scaffolds. Chitosan has also been shown to enhance mineral deposition during osteogenic differentiation of MSCs in vitro. The purpose of this review is to critically discuss the use of chitosan as a gene delivery vector with emphasis on its application in orthopedic tissue engineering.

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Section: Tissue Engineeringmentioning

confidence: 99%

Chitosan for Gene Delivery and Orthopedic Tissue Engineering Applications

2013

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“…Известно большое количество статей, в которых исследуются возможности формиро-вания матриц с помощью методов литографии. Эти методы позволяют формировать строго воспроизводи-мые запланированные массивы функ-ционально связанных макро-и нано-объектов [14,23]. Мы приверженцы данного направления, которое приме-нимо к любым конструкциям, любым полимерам и позволяет формиро-вать разнообразные высокоточные матрицы.…”

Section: рис 10unclassified

“…За последние три года опубликован ряд обзоров, посвя-щенных результатам использования нанотехнологий для решения задач инженерии тканей, прежде всего, костной [16,19,20,22]. Имеются также сообщения, что создаваемая нанопо-ристость на поверхности имплантатов (поры диаметром 30 нм) гарантирует их многолетнее использование [7,8,10,12,14,21]. Надо признать, что про-блема выращивания кости и формиро-вания тканеинженерных имплантатов является комплексной, для ее решения необходимо привлекать специалистов из различных областей, включая кле-точных биологов, специалистов в обла-сти биоматериаловедения, специали-стов по формированию гетерограниц и поверхностей, по изучению взаимо-действия клеток и материалов и т.д.…”

Section: рис 10unclassified

“…До недавнего време-ни на эту часть проблемы не обраща-ли должного внимания в связи с тем, что отсутствовали массовые методы формирования наноструктур. Однако быстрое развитие методов штамповой и импринт-микро-и импринт-нано-фотолитографии привело к форми-рованию целого направления в иссле-довании и практическом применении наноструктур в области регенератив-ной медицины [14,16,20].…”

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Создание Тканеинженерного Эквивалента Костной Ткани И Перспективы Его Использования В Травматологии И Ортопедии

Larionov¹,

Садовой²,

Самохин³

et al. 2014

Хирургия позвоночника 3-2014

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Objective. To present experimental prototypes of tissueengineered bone equivalent (TEBE) based on nanostructured bioresorbable synthetic polymer cellular matrices (BSPCMs) and osteogenic differentiated cells created to repair bone defects. Material and Methods. Nanostructured BSPCMs were developed and produced, which further served as the basis for creating TEBE. Cultured cells were transferred on the surface of nanostructured matrix, where patterns of their growth, expansion, and behavior were studied. Topography and surface properties of TEBE prototypes were investigated using methods of light-optical, scanning electron, and atomic force microscopy. Results. A possibility of creating experimental TEBE prototype based on BSPCM, which copies to the maximum extent the bone structure at the micro-and nanoscales is shown. Surface of the BSPCM was additionally nanostructured by formation of longitudinally oriented nano-trenches, to increase adhesion and osteoconductive properties. Conclusion. A strategy for creating nanostructured BSP-CM and TEBE was developed, and experimental prototypes suitable for further investigations to form biodegradable implants for needs of traumatology, orthopaedics, and spine medicine were produced. Key Words: matrix, scaffold, bone, tissue engineering, photolithography.Hir. Pozvonoc. 2014; (3):77-85. Цель исследования.Анализ экспериментальных об-разцов тканеинженерного эквивалента костной ткани (ТЭКТ) на основе наноструктурированных биорезор-бируемых синтетических полимерных клеточных матриц (БСПКМ) и остеогенных дифференцированных клеток для возмещения дефектов кости. Материал и методы. Разработаны и получены нано-структурированные БСПКМ, которые послужили ос-новой для создания ТЭКТ. Осуществлен перенос куль-тивируемых клеток на поверхность БСПКМ, изучены закономерности клеточного роста, экспансии, клеточ-ного поведения на поверхности БСПКМ. Выполнено изучение рельефа поверхности и заданных свойств об-разцов ТЭКТ с использованием методов светоопти-ческой, сканирующей электронной и атомно-силовой микроскопии. Результаты. Показана возможность создания экспери-ментального образца ТЭКТ на основе БСПКМ, который максимально копирует структуру костной ткани на ми-кро-и наноуровне. Поверхность БСПКМ имела допол-нительную наноструктурированность из-за нанесения продольных наноканавок, направленную на увеличение адгезивных и остеокондуктивных свойств. Заключение. Разработана стратегия создания БСПКМ и ТЭКТ, получены экспериментальные образцы, пригод-ные для дальнейших исследований с целью формирова-ния биодеградируемых имплантатов для нужд травмато-логии и ортопедии, вертебрологии.

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“…Furthermore, in order to get more efficient cell–material interaction and promote osteoconduction and osteoinduction during bone regeneration, fabrication of three‐dimensional (3D) structure in the biomaterials and surface property modification with specific growth factors, for example, BMPs, platelet‐derived growth factor, and transforming growth factor‐β, on the surface of the biomaterials have been further investigated and applied for the treatment of the bone defects in the animal model and/or clinic trials. The results suggest that the combination of the biomaterials with 3D structure and/or the surface modification with the growth factors bring great benefit for the treatment of bone defects .…”

mentioning

confidence: 95%

Implantation With New Three-Dimensional Porous Titanium Web for Treatment of Parietal Bone Defect in Rabbit

Guo

Iku

et al. 2013

Artificial Organs

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Titanium net (meshes) with excellent mechanical properties can promote bone compatibility and has been used as a repairing material for bone defects in clinical settings. In the present study, using spiral computed tomography (CT) and histomorphological techniques, we investigated the effect of a novel kind of titanium web with a three-dimensional (3D) porous structure on bone formation in rabbit skull (os parietal) defect. The images from the spiral CT scan demonstrate that the titanium web is completely fused with the surrounding bone tissue, even at the first month after implantation. The histomorphological findings show that different cells and tissues, including osseous tissue, connective tissue, and adipose cells, can easily grow into the 3D scaffold meshes of the titanium web, even in the center of the web and combine together as a whole body, suggesting that the titanium web possesses a very good biocompatibility, which is beneficial to the growth of bone tissue and promotes healing of the defected rabbit skull.

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Composite Scaffolds for Bone Tissue Regeneration

Cited by 4 publications

References 153 publications

Chitosan for Gene Delivery and Orthopedic Tissue Engineering Applications

Chitosan for Gene Delivery and Orthopedic Tissue Engineering Applications

Создание Тканеинженерного Эквивалента Костной Ткани И Перспективы Его Использования В Травматологии И Ортопедии

Implantation With New Three-Dimensional Porous Titanium Web for Treatment of Parietal Bone Defect in Rabbit

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