Composite chondroitin-6-sulfate/dermatan sulfate/chitosan scaffolds for cartilage tissue engineering

Chen, Yen‐Lin; Lee, Hsiao-Ping; Chan, Hing‐Yuen; Sung, Li‐Yu; Chen, Huang‐Chi; Hu, Yu‐Chen

doi:10.1016/j.biomaterials.2007.01.027

Cited by 104 publications

(58 citation statements)

References 28 publications

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“…Rapid degradation [170] [171]; [172]; [173]; [174] Alginate Aids re-differentiation of de-differentiated chondrocytes [66] In vivo injectable options [175] Abundant and low cost Certain cross-linkage can result in poor biocompatibility [184]; [185]; [186]; [187]; [188]; [189]; [182]; [190] Dermatan sulphate, [194]; [195] …”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Bioengineering of articular cartilage: past, present and future

Felimban

Moulton

et al. 2013

Regenerative Medicine

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The treatment of cartilage defects poses a clinical challenge owing to the lack of intrinsic regenerative capacity of cartilage. The use of tissue engineering techniques to bioengineer articular cartilage is promising and may hold the key to the successful regeneration of cartilage tissue. Natural and synthetic biomaterials have been used to recreate the microarchitecture of articular cartilage through multilayered biomimetic scaffolds. Acellular scaffolds preserve the microarchitecture of articular cartilage through a process of decellularization of biological tissue. Although promising, this technique often results in poor biomechanical strength of the graft. However, biomechanical strength could be improved if biomaterials could be incorporated back into the decellularized tissue to overcome this limitation.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Bioengineering of articular cartilage: past, present and future

Felimban

Moulton

et al. 2013

Regenerative Medicine

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“…polylactic acid, polyglycolic acid (Chu et al 1995;Giurea et al 2003;Ueki et al 2003;Hu & Athanasiou 2005), hydroxyapatite (Wu et al 2008), chitosan (Kim et al 2003;Chen et al 2007), and gelatin (Lien et al 2008), to design chondrocyteseeded or cell-free implants for articular cartilage repair. Some of these materials allow formation of the tissue to be repaired that resembles normal cartilage.…”

Section: Introductionmentioning

confidence: 99%

Collagen/hyaluronan membrane as a scaffold for chondrocytes cultivation

et al. 2009

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Rabbit chondrocytes were cultivated in vitro using the collagen/hyaluronan membrane. The membrane did not show any adverse effects on chondrocyte viability during in vitro cultivation. The inoculated cells grew without any negative changes. According to the histochemical analyses: (i) hematoxylin and eosin; (ii) safranin O; and (iii) rabbit anti-human collagen type II staining, the rabbit chondrocytes maintained their morphology and phenotype during in vitro cultivation. The collagen/hyaluronan membrane became more stable and stiffer after long time cultivation. The proliferation of the chondrocytes stabilised the structure of the membrane. The collagen/hyaluronan membrane is suitable material for the chondrocyte growth and could provide functional tissue-engineered scaffold for cartilage repair.

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“…The main drawbacks of chitosan are its poor biodegradability and hydrophilicity. Many chitosan-based composite scaffolds have been developed and shown to have beneficial qualities for tissue engineering (Bini et al, 2005;Chen et al, 2007). Since gelatin is capable of promoting cell adhesion and growth, here, we produced a chitosan/gelatin composite scaffold, with a view to enhancing cell attachment and proliferation.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of bone matrix gelatin/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering

Wang

Zhang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. This study was designed to evaluate bone matrix gelatin (BMG)/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering. Chondrocytes were isolated from costal cartilage of Sprague-Dawley rats and seeded on BMG/fibrin glue or chitosan/gelatin composite scaffolds. After different in vitro culture durations, the scaffolds were subjected to hematoxylin and eosin, Masson's trichrome, and toluidine blue staining, anti-collagen II and anti-aggrecan immunohistochemistry, and scanning electronic microscopy (SEM) analysis. After 2 weeks of culture, chondrocytes were distributed evenly on the surfaces of both scaffolds. Cell numbers and the presence of extracellular matrix components were markedly increased after 8 weeks of culture, and to a greater extent on the chitosan/gelatin scaffold. The BMG/fibrin glue scaffold showed signs of degradation after 8 weeks. Immunofluorescence analysis confirmed higher levels of collagen II and aggrecan using the chitosan/gelatin scaffold. SEM revealed that the majority of cells on the surface of the BMG/fibrin glue scaffold demonstrated a round morphology, while those in the chitosan/gelatin group had a spindle-like shape, with pseudopodia. Chitosan/gelatin scaffolds appear to be superior to BMG/ fibrin glue constructs in supporting chondrocyte attachment, proliferation, and biosynthesis of cartilaginous matrix components.

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Composite chondroitin-6-sulfate/dermatan sulfate/chitosan scaffolds for cartilage tissue engineering

Cited by 104 publications

References 28 publications

Bioengineering of articular cartilage: past, present and future

Bioengineering of articular cartilage: past, present and future

Collagen/hyaluronan membrane as a scaffold for chondrocytes cultivation

Evaluation of bone matrix gelatin/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering

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