Composição Química E Susceptibilidade Do Óleo Essencial De Óregano (Origanum Vulgare L., Família Lamiaceae) Frente À Cepas De Escherichia Coli, Staphylococcus Aureus E Salmonella Choleraesuis

Araújo, Luana Silva; Araújo, Renato Silva; Serra, Josilene Lima; Nascimento, Adenilde Ribeiro

doi:10.5380/cep.v33i1.43808

Cited by 10 publications

(11 citation statements)

References 2 publications

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“…Unlike our findings, Araújo et al (2015) identified 25 compounds in the oregano OE, with the two major ones being 4-terpineol and thymol, and with carvacrol not being detected as major compound. However, in the present study, samples of fresh oregano leaves were collected, while Araújo et al (2015) used dry leaves from the local market of São Luís, Maranhão. By assessing five commercial brands of oregano EO, Silva et al (2010) showed that the major component in all oils was carvacrol, corroborating our results.…”

Section: Resultscontrasting

confidence: 99%

In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates

Possamai

Santos

Silva

et al. 2019

SCA

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The pet industry is currently expanding and specializing mainly in the field of domestic felines. Problems related to antimicrobial resistance are frequent, and the use of essential oils (EOs) in animal feed has become a novel treatment strategy. Thus, the objective of this study was to assess the bacteriostatic activity of Brazilian lemon balm (Lippia alba), lemon grass (Cymbopogon citratus), and oregano (Origanum vulgare) in bacterial isolates from 12 samples of cat food sold in bulk. The EOs from fresh leaves of crops were obtained from the Medicinal Garden of Paranaense University, Umuarama, Paraná. Cat food samples were processed for identification of gram-positive and gram-negative microorganisms. The determination of the bacteriostatic activity of the EOs was performed by determination of the minimum inhibitory concentration (MIC) at dilutions of 2.5, 1.25, and 0.62 mg/mL. The diffusion disc technique was used to evaluate the resistance profile to the main antimicrobials used in the feline clinic and to analyze the effect of the association of these antimicrobials with the EOs studied. A total of 23 isolates were obtained, of which 16 were gram-negative and seven were gram-positive. As for the oil composition for L. alba, C. citratus, and O. vulgare, 40, 24, and 44 compounds were identified, respectively, with the major ones being geranial, geranial/?-citral, and carvacrol, respectively. Regarding MIC, no differences were found for any EOs tested. The lowest MIC value was obtained for the C. citratus EO (0.83 mg/mL) for two bacteria (coagulase-negative Staphylococcus and Corynebacterium kutscheri). The means of the inhibition halos for the 10 antimicrobials tested in association or not with one of the EOs for Klebsiella aerogenes, Proteus vulgaris, and Serratia rubidaea showed that, for S. rubidaea, the inhibition halo diameter (12.4 mm) was greater (p < 0.05) when amoxicillin was associated with the O. vulgare EO than the association of the same antibiotic with the C. citratus EO (11.0 mm). For K. aerogenes and P. vulgaris, there was no difference in inhibition halo diameter when EOs were included. In conclusion, L. alba, C. citratus, and O. vulgare EOs are effective in inhibiting the growth of gram-positive and gram-negative bacteria and can be added to cat food to replace chemical antimicrobials.

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Section: Resultscontrasting

confidence: 99%

In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates

Possamai

Santos

Silva

et al. 2019

SCA

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“…Chromatography revealed that the essential oils of the analyzed species presented a diverse group of major compounds, some of which have been widely cited in the literature. According to Araújo et al (2015), the antibacterial action of essential oils is directly related to their chemical composition. Additionally, Millezi et al (2014) reinforced that, in Brazil, studies have described the existence of different chemical characteristics between the essential oils of the same species that are grown in different geographic regions of the globe.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Essential Oils Against Pathogens of Importance in Caprine and Ovine Mastitis

et al. 2021

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Mastitis is an important infirmity that affects dairy goats and sheep flocks. Antimicrobials are widely used in mastitis therapy; however, the phenomenon of bacterial resistance has sparked interest in therapeutic alternatives, especially the research on essential oils from several plant species. Therefore, this study aimed to evaluate the antimicrobial activity of essential oils against pathogens of importance in caprine and ovine mastitis. The essential oils were obtained by hydrodistillation and the chemical composition was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests were performed to evaluate the antimicrobial activity of the essential oils (EO) of four plants. Fifteen bacterial isolates, previously identified as Staphylococcus spp. and Staphylococcus aureus, were used. The following plant species were used against the 15 bacterial isolates: Lippia origanoides “alecrim pimenta,” Lippia alba “erva cidreira,” Cymbopogon citratus “capim-santo,” and Ocimum basilicum “manjericão.” The first three essential oils presented antimicrobial activity against 100% of the isolates (15/15), with MICs of 560 µg mL-1, 1,173 µg mL-1, and 1,280 µg mL-1, and MBCs of 613 µg mL-1, 1,226 µg mL-1, and 1,333 µg mL-1, respectively. Nevertheless, the essential oil of O. basilicum, which was also tested, exerted no activity against any of the isolates. Carvacrol, citral, geraniol, and estragole were some of the major compounds found. These results can aid other studies that aim to use these essential oils as potential products for subsequent use in the treatment of mastitis in small ruminants.

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“…Estudos sugerem que as bactérias Gram-positivas são mais susceptíveis aos óleos essenciais, do que as Gram-negativas [3,23,24] . Isto ocorre provavelmente devido a complexidade estrutural da parede celular das Gram-negativas que apresentam uma membrana externa composta por lipopolissacarídeos, responsável por envolver a parede celular e proporcionar uma maior resistência, a qual restringe a difusão e dificulta a disseminação e acúmulo do óleo essencial na célula bacteriana [3,4,7,9,25] .…”

Section: Resultsunclassified

Efeito antimicrobiano e sinergístico de óleos essenciais sobre bactérias contaminantes de alimentos

Pombo

Ribeiro

Pinto

et al. 2018

Segur. Aliment. Nutr.

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Os óleos essenciais extraídos de plantas apresentam atividade antimicrobiana frente a diversos microrganismos, e são considerados como alternativa a conservação de alimentos. Este estudo testou o efeito antimicrobiano e sinergístico dos óleos essenciais de Eugenia caryophyllata (cravo) e Origanum vulgare (orégano) sobre bactérias contaminantes de alimentos: Escherichia coli, Salmonella entérica, Staphylococcus aureus e Bacillus cereus. O efeito antibacteriano de diferentes concentrações dos óleos essenciais, puros e em combinação, foi determinado pela técnica de difusão em ágar. O aumento da concentração de óleo essencial provocou um aumento significativo no efeito antimicrobiano (p≤0,05). As bactérias Gram-positivas foram mais sensíveis aos óleos essenciais, do que as Gram-negativas. O óleo de orégano apresentou um espectro de ação maior em relação ao cravo. As misturas binárias desses óleos na mesma proporção não apresentaram sinergismo. Enquanto as misturas nas maiores concentrações de orégano e menores de cravo apresentaram sinergismo e seu efeito antibacteriano foi potencializado.

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Composição Química E Susceptibilidade Do Óleo Essencial De Óregano (Origanum Vulgare L., Família Lamiaceae) Frente À Cepas De Escherichia Coli, Staphylococcus Aureus E Salmonella Choleraesuis

Cited by 10 publications

References 2 publications

In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates

In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates

Antibacterial Activity of Essential Oils Against Pathogens of Importance in Caprine and Ovine Mastitis

Efeito antimicrobiano e sinergístico de óleos essenciais sobre bactérias contaminantes de alimentos

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