Component Composition and Antimicrobial Activity of CO2 Extract of Portulaca oleracea, Growing in the Territory of Kazakhstan

Abstract

In the medicine of many countries, the use of herbal healing agents included a significant contribution to improving human health and well-being. Many antibiotics have been widely used to treat infectious diseases caused by various pathogenic bacteria. However, increased multidrug resistance has led to increased severity of diseases caused by bacterial pathogens. Bacteria remain the main causative agents of diseases that cause human death, even in the present day. This cause prompted scientists to investigate alternative new molecules against bacterial strains. The significant interest for the study is Portulaca oleracea L. (family Portulacaceae), a widespread annual plant used in folk medicine. Thus, the production and study of CO2 extract of Portulaca oleracea is an actual problem. Methods. Raw materials were collected from Almaty and Zhambyl regions (Southeast and South Kazakhstan) in phase flowering. Portulaca oleracea herb’s CO2 extract was obtained by subcritical carbon dioxide extraction (installation of carbon dioxide flow-through extraction- 5L). The Wiley 7th edition and NIST’02 library were used to identify the mass spectra obtained. The antimicrobial activity study was conducted by the micromethod of serial dilution and disco-diffuse method. Standard test strains of microorganisms were used: Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538-P, Candida albicans ATCC 10231, and Escherichia coli ATCC 8739. Results. The use of carbon dioxide extraction (further CO2 extract) is a promising direction of obtaining total medicinal substances containing biologically active substances, from fractions of volatile esters of various composition and functional purpose until a fraction of fatty acids and fat-soluble vitamins. In the current study, we obtained CO2 extract at subcritical conditions from aboveground organs of Portulaca oleracea and investigated the component composition for the first time. From 41 to 66 components were identified in the composition of Portulaca oleracea‘s CO2 extract. Studies of antimicrobial activity showed that CO2 extract of Portulaca oleracea had the expressed effect against clinically significant microorganisms such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Candida albicans. Conclusions. This study showed that CO2 extract of Portulaca oleracea’s raw material contained biological active compounds exhibiting a significant antimicrobial effect.

Chemical Composition and Antimicrobial Activity of Subcritical CO2 Extract of Lepidium latifolium L. (Brassicaceae)

“…It was also found hexane as a control does not show antimicrobial activity against cultures of microorganisms ( Figure 4 ). It was conditionally accepted that the diameter of the growth zone delay was over 15 mm for high activity, 10–15 mm for medium activity, and less than 10 mm for low activity [ 37 ].…”

Determination of the Chemical Composition and Antimicrobial Activity of Lavatera thuringiaca L. Medicinal Herb Material Extracted under Subcritical Conditions by the Liquid Carbon Dioxide Method

“…Also, this extract has a bacteriostatic effect against the clinical isolate of Staphylococcus haemolyticus and fungicidal activity against Candida albicans ATCC 10231 with a growth retardation zone of 22.0 ± 1.73. When interpreting the data, it was conditionally assumed that the diameter of the growth retardation zone of more than 15 mm proves high antimicrobial activity; 10-15 mm, average antimicrobial activity; and less than 10 mm, low antimicrobial activity [60]. e antimicrobial activity of the obtained extracts was compared with other extracts obtained by Serbian scientists Pavle Z. Mašković and others.…”

A review on bioactive phytochemicals, ethnomedicinal and pharmacological importance of Purslane (Portulaca oleracea L.)

“…El-Desouky et al (2021) found antifungal activities against three species of Aspergillus namely A. avus, A. ochraceus and A. parasiticus. Recently Tleubayeva et al (2021) showed that carbon dioxide extract of Purslane possess antibacterial activities against E. coli, S. aureus, B. subtilis, and antifungal activity against C. albicans.…”