Complexing capacity of the nutrient medium and its relation to inhibition of algal photosynthesis by copper

Gächter, René; Lum-Shue-Chan, K.; Chau, Y. K.

doi:10.1007/bf02502921

Cited by 41 publications

(16 citation statements)

References 19 publications

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“…Shuman and Michael (13) suggested that electrode processes at the mercury droplet may obscure the true nature of interactions of cupric ion with natural organic ligands. In addition, Gachter et al (14) suggested that a chemical measurement of natural complexation capacity of a water sample does not by itself guarantee that an equivalent amount of copper can be tolerated without an adverse effect on phytoplankton production.…”

Section: Introductionmentioning

confidence: 99%

Copper complexation capacity of marine water samples from southern California

Srna¹,

Garrett²,

Miller³

et al. 1980

Environ. Sci. Technol.

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The ability of a variety of natural water samples from southern California to complex cupric ion was measured by using anodic stripping voltammetry (ASV) and a technique employing algal bioassay. Samples investigated ranged from oceanic waters to a freshwater lake which drained into a coastal lagoon. According to ASV results, complexation capacity ranged from undetectable to 0.93 pM Cu2+/L. The difference between ECjo values obtained from bioassays using a 0.45-pm filtered and ultrafiltered ( G O O M,) water was used to measure the reduction in free cupric ion by high-molecular-weight compounds. Cupric ion EC50 values differed by up to 2.3 pM between filtered and ultrafiltered samples. The correlation between results using the titration method and the bioassay technique was 0.993. Quantitative differences between chemical measures of complexation and biological effect indicated the importance of verifying the accuracy of chemical techniques used to measure complexation capacity. Results of the survey suggested that coastal lagoons provide a useful test site for study of the dynamics of complexing compounds in the marine environment.

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Section: Introductionmentioning

confidence: 99%

Copper complexation capacity of marine water samples from southern California

Srna¹,

Garrett²,

Miller³

et al. 1980

Environ. Sci. Technol.

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“…Most work has involved laboratory experiments rather than field studies, and comparatively little is known about the complexation capacities and metal-binding specificities of natural ligands in freshwater ecosystems generally or the North American Great Lakes in particular. Pioneering work in this respect was conducted originally by Chau et al (1970) and Gächter et al (1973). The latter authors demonstrated that despite apparent excess complexing capacity for Cu of several hundred nanomoles per litre in waters from Lake Ontario, the Niagara River, and Hamilton Harbor, additions of as little as 100 nmol·L -1 Cu reduced carbon fixation substantially.…”

Section: Introductionmentioning

confidence: 98%

Copper inhibition of phytoplankton in Saginaw Bay, Lake Huron

Lehman¹,

Bazzi²,

Nosher³

et al. 2004

Can. J. Fish. Aquat. Sci.

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Field surveys and bioassays during 1999 and 2000 demonstrated trace metal effects on the phytoplankton of inner and outer regions of Saginaw Bay, Lake Huron. Addition of as little as 1 µg·L -1 copper suppressed algal biomass, measured as particulate chlorophyll a, compared with control treatments, additions of ethylenediaminetetraacetic acid, or additions of carrier water alone. Suppression effects of copper, added alone or in combination with cadmium, lead, and thallium, were evident in inner and outer regions of Saginaw Bay during both spring and summer when phytoplankton communities were composed alternatively of diatoms and cyanobacteria. Experimental treatments were conducted in parallel with measurements of metal concentrations and metal complexation capacities of lake water collected by ultraclean trace metal techniques. Despite apparent overchelation of dissolved copper by organic ligands in Saginaw Bay, additions of a few tens of nanomoles of copper per litre strongly reduces algal biomass compared with control treatments. These results suggest that metal concentrations in some Great Lakes waters in equilibrium with natural chelators may be above the essential requirements and potentially at levels of incipient toxicity to the native phytoplankton communities.Résumé : Des inventaires de terrain et des essais biologiques en 1999 et 2000 ont démontré les effets des métaux en traces sur le phytoplancton des régions intérieure et extérieure de la baie de Saginaw au lac Huron. L'addition d'une quantité aussi faible que 1 µg·L -1 de cuivre supprime la biomasse des algues, mesurée en tant que chlorophylle a particulaire par comparaison avec les témoins qui ont reçu des additions d'acide éthylènediamine tétra-acétique ou des additions de l'eau de dilution seule. Les effets de suppression du cuivre, ajouté seul ou en combinaison avec du cadmium, du plomb et du thallium, sont apparents dans les régions intérieure et extérieure de la baie de Saginaw, tant au printemps qu'à l'été, à des moments où les communautés phytoplanctoniques sont composées respectivement de diatomées et de cyanobactéries. Les expériences ont été réalisées en parallèle à des dosages des concentrations de métaux et des mesures du pouvoir de complexation des métaux de l'eau du lac récoltée par des techniques ultra-propres pour l'analyse des métaux en traces. Malgré une chélation excessive du cuivre dissous par les ligands organiques dans la baie de Saginaw, l'addition de quelques dixs de nanomoles de cuivre par litre réduit considérablement la biomasse des algues, par comparaison avec les témoins. Ces résultats indiquent que les concentrations de métaux dans certaines eaux des Grands Lacs en équilibre avec les agents de chélation naturels peuvent dépasser les besoins essentiels des communautés indigènes de phytoplancton et se situer potentiellement à des concentrations correspondant à un début de toxicité.[Traduit par la Rédaction] Lehman et al. 1880

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“…Phytoplankton growth experiments by Barber (3) demonstrated significant response to small additions of both metals and chelators. These and stmilar observations led G~chter, Lum-Shue-Chan and Chau (4) to conclude that the free copper ion and its inorganic complexes are more toxic than are its organic ccmplexes.…”

mentioning

confidence: 99%

Trace metal ion activities from liquid-liquid partitioning measurements

Kennish¹

2000

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Elucidation of the chemical speciation of trace metals ~n the natural aquatic environment will lead to a better understanding of thei~ distribution and ecological effects. One approach which can provide useful information about the chemical reactivity of metal ions is the measurement of their activity. Phase equilibrium methods are required and liquid-liquid partition equilib"da are applicable. This study 2 utilized model systems to demonstrate this applicability.The partitioning of copper(II) ions as a chelate of acetylacetone was used to determine the trace activity coefficients of the copper(II) electrolyte in the CU(N03)2-HN03-KN03, Cu(N03)2-HCl04-NaCl04 and CuC1 2 -HCl-KCl systems over a wide range of ionic strengths (~).By careful control of pH and acetylacetone concentration only 1-3% of the metal ion was extracted. Under these conditions the amount extracted is proportional to the activity. The concentration of the bis(Acetylacetonato) Copper(II) was determined in the organic phase by spectrophotometric and atomic absorption methods but any convenient concentration technique could be used to measure the amount extracted.A comparison of activity measurements by liquid-liquid partitioning was made with electrochemical measurements by utilizing a copper ion selective electrode. The significantly lower activity coefficient values obtained by the electrochemical method were explained in terms of the liquid junction potential and the necessity for extrathermodynamic approaches to single ion activities.Potential application of the liquid-liquid partitioning method to the determination of trace activity coefficients in natural aquatic systems was demonstrated by extension of the method to measurements in copper(II) amino acid solutions at ~ = 0.001 and ~ = 0.723. The ionic strength adjustments in this case were made with NaCl. A significant difference in the free copper (II) ion activity was observed between solutions of copper(II) glycinate and copper(II) alaninate under identical conditions of metal and ligand concentrations, pH and ionic strength. Lum-Shue-Chan and Chau (4) to conclude that the free copper ion and its inorganic complexes are more toxic than are its organic ccmplexes.They estimated that ionic copper becomes toxic to planktonic algae at concentrations of approxtmate1y 10-10 M. Stiff (5) and Pagenkopf, Russo and Thurston (6) indicated that reduced copper toxicity results from bicarbonate alkalinity through formation of carbonate complexes or precipitates. Sylva (7) showed that the influence of organic complexing agents such as humic and fulvic acid cannot be overlooked. 2More recently, detailed studies utilizing equilibrium calculations have provided increased evidence that copper toxicity is indeed related to the activity of the free copper ion. Sunda and Guillard (8), utilizing cultures of Thalassiosira pseudonana and Nannochlous atomus, found that both growth rate inhibition and the copper content of the cells were related to the activity of the cupric ion. The growth rates of Thalassios...

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Complexing capacity of the nutrient medium and its relation to inhibition of algal photosynthesis by copper

Cited by 41 publications

References 19 publications

Copper complexation capacity of marine water samples from southern California

Copper complexation capacity of marine water samples from southern California

Copper inhibition of phytoplankton in Saginaw Bay, Lake Huron

Trace metal ion activities from liquid-liquid partitioning measurements

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