2002
DOI: 10.1074/jbc.m200304200 View full text |Buy / Rent full text
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Abstract: RNA recognition motifs (RRMs) are characterized by highly conserved regions located centrally on a ␤-sheet, which forms the RNA binding surface. Variable flanking regions, such as the loop connecting ␤-strands 2 and 3, are thought to be important in determining the RNAbinding specificities of individual RRMs. The N-terminal RRM of the spliceosomal U1A protein mediates binding to an RNA hairpin (U1hpII) in the U1 small nuclear RNA. In this complex, the ␤ 2 -␤ 3 loop protrudes through the 10-nucleotide RNA loop.… Show more

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“…2). In support of this, we have previously observed no binding to a U1hpII RNA with a reversedloop sequence, in which six of the seven target nucleotides are mutated (Katsamba et al 2001(Katsamba et al , 2002aLaw et al 2005). Kinetic analysis of the interaction of U1A with the +5 bp and +10 bp RNA targets showed a small increase in the association rate ( Fig.…”
Section: Lengthening the Rna Stem Has Minor Kinetic Effectssupporting
“…Presumably, this allows the formation of sufficient close-range interactions to allow the two molecules to begin association. Based on our previous work, this involves, among others, electrostatic interactions between the RNA and Lys20, 22, and 50, and the stacking of Tyr13 on C5 (Katsamba et al 2001(Katsamba et al , 2002aLaw et al 2005Law et al , 2006. Thus, surprisingly, the unstructured nature of the target sequence is not a major factor.…”
Section: Increasing Loop Length Results In Faster Association While Dmentioning
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“…The Ser140 residue is located in the helix b2-helix b3 loop that is involved in the binding to U1 RNA according to a model of the homologous RRM in the spliceosomal U1A protein. 30 Therefore, an intriguing possibility arises that the phosphorylation of Ser140, by a so far unidentified kinase, plays a role in the regulation of the U1-70K protein during mRNA splicing, by affecting its binding to U1 RNA. The increase in phosphorylation of Ser140 we observed early during apoptosis could then be associated with a change in mRNA splicing activity required for the progression of apoptosis.…”
Section: Discussionmentioning
“…The complex between the spliceosomal protein U1A and its target on the U1 small nuclear RNA has served as a model system for many studies. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] In this complex, the N-terminal RNA recognition motif of U1A interacts in a sequence-specific manner with stem/loop II (U1SLII) of the U1 small nuclear RNA ( Figure 1). Both experimental and computational studies have probed determinants of the binding affinity, and a number of experimental studies 1,7,9,10,[13][14][15] have been carried out to dissect the binding rate.…”
Section: Introductionmentioning