Complex regional pain syndrome: A case report and review of the literature

Alkali, Nura H.; AlTahan, Abdulrahman; Al-Majed, Mohammad; Altahan, Husam

doi:10.4103/aam.aam_23_19

Cited by 5 publications

(2 citation statements)

References 9 publications

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“…The method also reduces the chances of persistent neuropathic pains, complex regional pain syndrome, double sticking, anxiety and fainting resulted from venipuncture during sample collection. 24,25 Similarly, the volume of blood required to obtain RNA and DNA samples will be reduced. These are advantageous especially to patients with low blood count-related diseases such as sickle cell disease, thalassemia, leukemia, aplastic anemia, iron and vitamin deficiency, as well as hemophobic subjects.…”

Section: Discussionmentioning

confidence: 99%

Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique

et al. 2022

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Objectives: Extraction of DNA and RNA is the first step in genomics and transcriptomics studies. Phenol-chloroform method for DNA extraction has been the widely used method. However, this method is relatively expensive and time-consuming. The objective of the present study was to validate a cost and time-effective protocol that will reduce the burden of molecular biology-based research and make a difference in laboratories with limited resources. Methods: A comparative study was conducted at Syed Qamer Alam Research Laboratory, Shifa College of Medicine; from February, 2021 to August, 2021. TRIzolTM method was used to extract RNA from blood samples of coronary artery disease patients and remnant was used to extract DNA. The quantity, purity and integrity of the extracted DNA by both methods (TRIzol and phenol-chloroform) was examined. PCR product amplification was performed with thrombomodulin (THBD) gene to validate the characteristic of the extracted DNA and its efficiency for downstream experiments. Results: The DNA yield in the TRIzolTM method was three-fold higher than phenol chloroform method. Both methods showed intact genomic DNA on the agarose gel, and extracted DNA was efficient for PCR amplification. Conclusion: The TRIzolTM method for RNA and DNA co-extraction is fast, simple and economical technique. So, it can be adopted for routine molecular biology analyses in limited resources setup. doi: https://doi.org/10.12669/pjms.38.7.5509 How to cite this:Dandare A, Rafiq M, Liaquat A, Khan MJ. Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique. Pak J Med Sci. 2022;38(7):---------. doi: https://doi.org/10.12669/pjms.38.7.5509 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 99%

Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique

et al. 2022

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“…Complex regional pain syndrome (CRPS) is a rare neuropathic pain disorder that is associated with severe pain [205]. CPRS typically occurs after a regional injury and presents nociceptive, vascular, and bone distal limb changes that surpass the predicted clinical course of the initiating injury in both severity and duration, often resulting in severe motor dysfunction and disability.…”

Section: Aliamides In Peripheral Neuropathic Painmentioning

confidence: 99%

ALIAmides Update: Palmitoylethanolamide and Its Formulations on Management of Peripheral Neuropathic Pain

D’Amico

Impellizzeri

Cuzzocrea

et al. 2020

IJMS

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Neuropathic pain results from lesions or diseases of the somatosensory nervous system and it remains largely difficult to treat. Peripheral neuropathic pain originates from injury to the peripheral nervous system (PNS) and manifests as a series of symptoms and complications, including allodynia and hyperalgesia. The aim of this review is to discuss a novel approach on neuropathic pain management, which is based on the knowledge of processes that underlie the development of peripheral neuropathic pain; in particular highlights the role of glia and mast cells in pain and neuroinflammation. ALIAmides (autacoid local injury antagonist amides) represent a group of endogenous bioactive lipids, including palmitoylethanolamide (PEA), which play a central role in numerous biological processes, including pain, inflammation, and lipid metabolism. These compounds are emerging thanks to their anti-inflammatory and anti-hyperalgesic effects, due to the down-regulation of activation of mast cells. Collectively, preclinical and clinical studies support the idea that ALIAmides merit further consideration as therapeutic approach for controlling inflammatory responses, pain, and related peripheral neuropathic pain.

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Complex Regional Pain Syndrome: Evidence-Based Advances in Concepts and Treatments

Limerick

Christo

Tram

et al. 2023

Curr Pain Headache Rep

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Complex regional pain syndrome: A case report and review of the literature

Cited by 5 publications

References 9 publications

Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique

Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique

ALIAmides Update: Palmitoylethanolamide and Its Formulations on Management of Peripheral Neuropathic Pain

Complex Regional Pain Syndrome: Evidence-Based Advances in Concepts and Treatments

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