Complex pattern of the myelo-monocytic differentiation antigens MRP8 and MRP14 during chronic airway inflammation

Roth, Johannes; Teigelkamp, Stefan; Wilke, Martina; Grün, Lothar; Tümmler, Burckhardt; Sorg, Clemens

doi:10.1016/s0171-2985(11)80259-7

Cited by 93 publications

(85 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8,9,41 Expression of MRP8 and MRP14 in vivo correlates well with disease activity in different models of inflammation in mice as well as in various kinds of human inflammatory diseases. 11,12,14,16,17,25 Analyzing expression of MRP8 and MRP14 in IM we found two different phenotypes of infil- trating monocytes/macrophages with a close association of highly MRP8/MRP14-expressing monocytes with destructed muscle fibers in DM, PM, and IBM. Since both proteins are secreted by monocytes at local sites of inflammation via a so-called alternative pathway of secretion 16,34 we looked for direct effects of extracellular MRP8/MRP14 on myocytes.…”

Section: Discussionmentioning

confidence: 83%

“…12,34 Since presence of MRP8/MRP14 complexes was such a characteristic feature in all three types of IM and since it was associated with muscle necrosis, we wondered whether MRP8/MRP14 would have a direct effect on striated muscle cells. To investigate direct effects of MRP8/MRP14 complexes on proliferation of C2C12 myoblasts in vitro, cells were cultured for 24 hours with varying concentrations of MRP8/MRP14 and then stained with anti-Ki67 (Figure 2).…”

Section: Mrp8/mrp14 Complex Inhibits Proliferation Of C2c12 Myoblastsmentioning

confidence: 99%

“…13,23,24 In addition, both proteins are specifically released by monocytes during the course of inflammatory reactions, and serum concentrations of MRP8 and MRP14 have been shown to correlate well with the activity of inflammatory reactions in various human diseases. 11,12,14,16,17,25 In the present study we investigated the expression and local distribution of MRP8 and MRP14 in different inflammatory muscle disorders (DM, PM, and IBM) as well as their effects on muscle cells in vitro. In all three kinds of IM we found a clear correlation between infiltration of MRP8/ MRP14-expressing monocytes and the inflammatory destruction of muscle fibers.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Expression of Calcium-Binding Proteins MRP8 and MRP14 in Inflammatory Muscle Diseases

Seeliger

Vogl

Engels

et al. 2003

The American Journal of Pathology

Self Cite

View full text Add to dashboard Cite

The pathophysiological role of infiltrating macrophages and their subtypes in idiopathic inflammatory myopathies such as dermatomyositis, polymyositis, and inclusion body myositis is not fully clear. Monocytes exhibit various phenotypes with different functional properties such as release of pro-or anti-inflammatory mediators. Expression of myeloid-related proteins MRP8 and MRP14, two calcium-binding S100-proteins, characterizes a proinflammatory subtype of macrophages. We immunohistochemically investigated expression of MRP8 and MRP14 in muscle biopsies of 33 patients with dermatomyositis, polymyositis, and inclusion body myositis. We found a clear association of expression of MRP8 and MRP14 by infiltrating macrophages with degeneration of myofibers. Because MRP8 and MRP14 are secreted by activated macrophages we investigated if these proteins would have direct extracellular effects on myocytes. We found that the purified MRP8/MRP14 complex inhibited proliferation and differentiation of C2C12 myoblasts and that it induced apoptosis via activation of caspase-3 in a time-and dose-dependent manner. These results indicate that in the course of inflammatory myopathies, activated macrophages can promote destruction and impair regeneration of myocytes via secretion of MRP8/MRP14. Myositis is a term describing muscle inflammation independent of its etiology. The heterogeneous group of acute and chronic idiopathic inflammatory myopathies (IM) is histologically characterized by signs of destruction and partial regeneration of inflamed muscle fibers.

show abstract

Section: Discussionmentioning

confidence: 83%

Section: Mrp8/mrp14 Complex Inhibits Proliferation Of C2c12 Myoblastsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Expression of Calcium-Binding Proteins MRP8 and MRP14 in Inflammatory Muscle Diseases

Seeliger

Vogl

Engels

et al. 2003

The American Journal of Pathology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The presence of S100A8, S100A9, and S100A8/A9 has been associated with bacterial infections in humans and mice (12,15,37). However, it is not clear whether this was a consequence of inflammation, or if it contributed actively to the development of the innate immune response.…”

Section: Discussionmentioning

confidence: 99%

“…The high levels of serum S100A8/A9 in chronic inflammatory pathologies such as rheumatoid arthritis and inflammatory bowel disease (13,35,36), as well as during bronchitis and tuberculosis (12,37), suggest that they might play a role in inflammatory reactions. However, clear evidence regarding their involvement in leukocyte migration remains undiscovered.…”

mentioning

confidence: 99%

Blockade of S100A8 and S100A9 Suppresses Neutrophil Migration in Response to Lipopolysaccharide

Vandal

Rouleau

Boivin

et al. 2003

The Journal of Immunology

200

169

View full text Add to dashboard Cite

Recently, proinflammatory activities had been described for S100A8 and S100A9, two proteins found at inflammatory sites and within the neutrophil cytoplasm. In this study, we investigated the role of these proteins in neutrophil migration in vivo in response to LPS. LPS was injected into the murine air pouch, which led to the release of S100A8, S100A9, and S100A8/A9 in the pouch exudates that preceded accumulation of neutrophils. Passive immunization against S100A8 and S100A9 led to a 52% inhibition of neutrophil migration in response to LPS at 3 h postinjection. Injection of LPS was also associated with an increase in peripheral blood neutrophils and the presence in serum of S100A9 and S100A8/A9. Intravenous injection of S100A8, S100A9, or S100A8/A9 augmented the number of circulating neutrophils and diminished the number of neutrophils in the bone marrow, demonstrating that S100A8 and S100A9 induced the mobilization of neutrophils from the bone marrow to the blood. Finally, passive immunization with anti-S100A9 inhibited the neutrophilia associated with LPS injection in the air pouch. These results suggest that S100A8 and S100A9 play a role in the inflammatory response to LPS by inducing the release of neutrophils from the bone marrow and directing their migration to the inflammatory site.

show abstract

EFHand

Fagan

Nelson

Chazin

2002

Wiley Encyclopedia of Molecular Medicine

View full text Add to dashboard Cite

Complex pattern of the myelo-monocytic differentiation antigens MRP8 and MRP14 during chronic airway inflammation

Cited by 93 publications

References 21 publications

Expression of Calcium-Binding Proteins MRP8 and MRP14 in Inflammatory Muscle Diseases

Expression of Calcium-Binding Proteins MRP8 and MRP14 in Inflammatory Muscle Diseases

Blockade of S100A8 and S100A9 Suppresses Neutrophil Migration in Response to Lipopolysaccharide

EFHand

Contact Info

Product

Resources

About