Complete Nucleotide Sequence of the IncN Plasmid Encoding IMP-6 and CTX-M-2 from Emerging Carbapenem-Resistant Enterobacteriaceae in Japan

Kayama, Shizuo; Shigemoto, Norifumi; Kuwahara, Ryuichi; Oshima, Koichiro; Hirakawa, Hideki; Hisatsune, Junzo; Jové, Thomas; Nishio, Hisaaki; Yamasaki, Katsutoshi; Wada, Yasunao; Ueshimo, Takeshi; Miura, Taito; Sueda, Taijiro; Onodera, Makoto; Yokozaki, Michiya; Hattori, Masahira; Ohge, Hiroki; Sugai, Motoyuki

doi:10.1128/aac.04759-14

Cited by 42 publications

(45 citation statements)

References 19 publications

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“…Other plasmid replicon groups, such as IncN, IncP, IncX, IncU, IncI, A/C, IncR, and L/M, are also occasionally reported in KPC‐producing K. pneumoniae . Moreover, these plasmid replicon groups are also associated with K. pneumoniae strains producing other carbapenemases.…”

Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

“…The IncN plasmid type is also of broad host range, with high transmission efficiency. These plasmids are also important in the dissemination of carbapenemase genes, including bla KPC , bla NDM , bla IMP , and bla VIM (Table ) . Within this group, three subgroups with similar plasmid scaffolds but less similarity in backbone sequences have been described: IncN1 (R46), IncN2 (p271A), and IncN3 (pN‐Cit); these characteristics might be the reason for their stability and success in disseminating multiple carbapenemases.…”

Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

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Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Kopotsa

Sekyere

Mbelle

2019

Annals of the New York Academy of Sciences

171

178

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Carbapenem‐resistant Enterobacteriaceae (CRE) have been listed by the WHO as high‐priority pathogens owing to their high association with mortalities and morbidities. Resistance to multiple β‐lactams complicates effective clinical management of CRE infections. Using plasmid typing methods, a wide distribution of plasmid replicon groups has been reported in CREs around the world, including IncF, N, X, A/C, L/M, R, P, H, I, and W. We performed a literature search for English research papers, published between 2013 and 2018, reporting on plasmid‐mediated carbapenem resistance. A rise in both carbapenemase types and associated plasmid replicon groups was seen, with China, Canada, and the United States recording a higher increase than other countries. blaKPC was the most prevalent, except in Angola and the Czech Republic, where OXA‐181 (n = 50, 88%) and OXA‐48–like (n = 24, 44%) carbapenemases were most prevalent, respectively; blaKPC‐2/3 accounted for 70% (n = 956) of all reported carbapenemases. IncF plasmids were found to be responsible for disseminating different antibiotic resistance genes worldwide, accounting for almost 40% (n = 254) of plasmid‐borne carbapenemases. blaCTX‐M, blaTEM, blaSHV, blaOXA‐1/9, qnr, and aac‐(6′)‐lb were mostly detected concurrently with carbapenemases. Most reported plasmids were conjugative but not present in multiple countries or species, suggesting limited interspecies and interboundary transmission of a common plasmid. A major limitation to effective characterization of plasmid evolution was the use of PCR‐based instead of whole‐plasmid sequencing–based plasmid typing.

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Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Kopotsa

Sekyere

Mbelle

2019

Annals of the New York Academy of Sciences

171

178

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“…This phenomenon is especially important in Japan because the most prevalent CPE in Japan are IMP-1 and IMP-6- types, which show low-level resistance to carbapenems. Furthermore, IMP-6-type isolates are generally susceptible to imipenem, but resistant to meropenem [10, 11]. Therefore, IMP-type CPE is occasionally misidentified in laboratory examinations and is referred to as ‘stealth-type CPE’ to draw attention to this type of CPE [11].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, IMP-6-type isolates are generally susceptible to imipenem, but resistant to meropenem [10, 11]. Therefore, IMP-type CPE is occasionally misidentified in laboratory examinations and is referred to as ‘stealth-type CPE’ to draw attention to this type of CPE [11]. Several reports indicated difficulties in screening for OXA-48-type isolates, which are widely known to display low-level resistance [12].…”

Section: Introductionmentioning

confidence: 99%

Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens

et al. 2017

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BackgroundIdentification of carbapenemase-producing Enterobacteriaceae (CPE) in faecal specimens is challenging. This fact is particularly critical because low-level carbapenem-resistant organisms such as IMP-producing CPE are most prevalent in Japan. We developed a modified selective medium more suitable for IMP-type CPE.MethodsFifteen reference CPE strains producing different types of β-lactamases were used to evaluate the commercially available CHROMagar KPC and chromID CARBA as well as the newly prepared MC-ECC medium (CHROMagar ECC supplemented with meropenem, cloxacillin, and ZnSO4) and M-ECC medium (CHROMagar ECC supplemented with meropenem and ZnSO4). A total of 1035 clinical samples were then examined to detect CPE using chromID CARBA and M-ECC medium.ResultsAll tested strains producing NDM-, KPC-, and OXA-48-carbapenemases were successfully cultured in the media employed. Although most of the IMP-positive strains did not grow in CHROMagar KPC, chromID CARBA, or MC-ECC, all tested strains grew on M-ECC. When faecal samples were applied to the media, M-ECC medium allowed the best growth of IMP-type CPE with a significantly higher sensitivity (99.3%) than that of chromID CARBA (13.9%).ConclusionsM-ECC medium was determined as the most favourable selective medium for the detection of IMP-type CPE as well as other types of CPE.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-017-2312-1) contains supplementary material, which is available to authorized users.

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“…They also contribute to the transfer of clinically important antibiotic resistance genes (e.g. encoding carbapenem resistance in Klebsiella pneumoniae) (1). The clinical isolate R46 (2) as well as its deletion variant pKM101 are the most studied model systems (3).…”

mentioning

confidence: 99%

Structural Analysis and Inhibition of TraE from the pKM101 Type IV Secretion System

Casu

Smart

Hancock

et al. 2016

Journal of Biological Chemistry

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Gram-negative bacteria use type IV secretion systems (T4SSs) for a variety of macromolecular transport processes that include the exchange of genetic material. The pKM101 plasmid encodes a T4SS similar to the well-studied model systems from Agrobacterium tumefaciens and Brucella suis. Here, we studied the structure and function of TraE, a homolog of VirB8 that is an essential component of all T4SSs. Analysis by X-ray crystallography revealed a structure that is similar to other VirB8 homologs but displayed an altered dimerization interface. The dimerization interface observed in the X-ray structure was corroborated using the bacterial two-hybrid assay, biochemical characterization of the purified protein, and in vivo complementation, demonstrating that there are different modes of dimerization among VirB8 homologs. Analysis of interactions using the bacterial two-hybrid and cross-linking assays showed that TraE and its homologs from Agrobacterium, Brucella, and Helicobacter pylori form heterodimers. They also interact with heterologous VirB10 proteins, indicating a significant degree of plasticity in the protein-protein interactions of VirB8-like proteins. To further assess common features of VirB8-like proteins, we tested a series of small molecules derived from inhibitors of Brucella VirB8 dimerization. These molecules bound to TraE in vitro, docking predicted that they bind to a structurally conserved surface groove of the protein, and some of them inhibited pKM101 plasmid transfer. VirB8-like proteins thus share functionally important sites, and these can be exploited for the design of specific inhibitors of T4SS function.

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Complete Nucleotide Sequence of the IncN Plasmid Encoding IMP-6 and CTX-M-2 from Emerging Carbapenem-Resistant Enterobacteriaceae in Japan

Cited by 42 publications

References 19 publications

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens

Structural Analysis and Inhibition of TraE from the pKM101 Type IV Secretion System

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