Complete mitochondrial genome of the pink bollworm Pectinophora gossypiella (Lepidoptera: Gelechiidae)

Abstract

Pectinophora gossypiella (Lepidoptera: Gelechiidae) is a key pest in many cotton-growing countries of the world. In this study, the complete mitochondrial (mt) genome of the pink bollworm P. gossypiella was determined, which is 15,202 bp in length (GenBank accession number: KM225795) containing 37 typical animal mitochondrial gene and an A + T-rich region. The gene order of P. gossypiella mtDNA was different from the insect ancestral gene order in the translocation of trnM, as shared by previously sequenced lepidopteran mtDNAs. The protein-coding genes (PCGs) have typical mitochondrial start codons ATN, with the exception of COI, Nad5, which uses the start codons CGA, GTT. Eight PCGs stop with complete termination codons (TAA), whereas five PCGs use incomplete stop codon T. All of the tRNA genes had typical cloverleaf secondary structures except for trnS1(AGN), in which the dihydrouridine (DHU) arm did not form a stable stem-loop structure. Like other insects, the control region is located between rrnS and trnM with a length of 309 bp and an A + T content of 94.8%, which is the most AT-rich region and comparatively simple, with little evidence of long tandem repeats, but harbors a conserved structure combining the motif ATAGA and a 18-bp poly-T stretch.

Complete mitochondrial genome of the gelechioidHieromantis kurokoi(Lepidoptera: Stathmopodidae)

“…The gene arrangement of H. kurokoi is identical to that of other ditrysian Lepidoptera, including gelechioids, that have the order trnM-trnI-trnQ (where the underline indicates a gene inversion) between the A þ T-rich region and ND2 (Kim et al 2011;Park et al 2016;Timmermans et al 2014;Zhao et al 2016), instead of the ancestral trnI-trnQ-trnM order found in the majority of insects (Boore 1999).…”

“…and Nepticuloidea (Stigmella roborella) were included as outgroups. GenBank accession numbers are as follows: Urodus decens, KJ508062 (Timmermans et al 2014); Adoxophyes honmai, DQ073916 (Lee et al 2006); Ethmia eupostica, KJ508047 (Timmermans et al 2014); Perimede sp., KJ508041 (Timmermans et al 2014); Endrosis sarcitrella, KJ508037 (Timmermans et al 2014); Promalactis suzukiella, KM875542 (Park et al 2014); Oegoconia novimundi, KJ508036 (Timmermans et al 2014); Atrijuglans hetaohei, KT581634 (Unpublished); Pectinophora gossypiella, KM225795 (Zhao et al 2016); Tineola bisselliella, KJ508045 (Timmermans et al 2014); Phyllonorycter froelichiella, KJ508048 (Timmermans et al 2014); Astrotischeria sp., KJ508056 (Timmermans et al 2014); and Stigmella roborella, KJ508054 (Timmermans et al 2014). …”

“…Nevertheless, prior to this study, only seven mitochondrial genomes (mitogenomes), representing six of the 19 families, have been sequenced (Park et al 2016;Timmermans et al 2014;Zhao et al 2016). Thus, more species from a diverse taxonomic group will be essential for mitogenome-based phylogenetic studies.…”

Complete mitochondrial genome of the gelechioidStathmopoda auriferella(Lepidoptera: Stathmopodidae)

“…to 15,408 bp (Oegoconia novimundi) (Timmermans et al 2014;Park et al 2016a, b;Zhao et al 2016). The gene arrangement of S. auriferella was identical to that of other ditrysian Lepidoptera, including gelechioids.…”

“…It is the second most species-rich group of Lepidoptera (van Nieukerken et al 2011) that is important to attaining a better understanding of higher-level phylogeny van Nieukerken et al 2011). Nevertheless, prior to this study, only eight mitochondrial genomes (mitogenomes) have been sequenced (Timmermans et al 2014;Park et al 2016a, b;Zhao et al 2016). Greater focus on additional species from a diverse taxonomic group is essential to conduct comprehensive mitogenome-based phylogenetic studies on Lepidoptera (including Gelechioidea).…”