Complete maternal mitochondrial genome of the freshwater mussel Cuneopsis celtiformis (Bivalvia: Unionidae)

Abstract

The genus Cuneopsis Simpson, 1900 comprises seven valid species, and Cuneopsis celtiformis (Heude, 1874) is the type species of this genus. Previous phylogenetic studies using complete mitochondrial genomes showed that Cuneopsis was not monophyletic, but the result was hampered by incomplete species sampling and lack of the type species of this genus. In this study, we collected C. celtiformis from the type locality and determined its complete maternal mitochondrial genome. This mitogenome is 15,922 bp in length and contains 14 protein-coding genes (including one F- orf ), two rRNA genes, 22 tRNA genes, and 1 putative control region. Our mitochondrial phylogenomic analysis confirms that currently recognized genus Cuneopsis is polyphyletic, and C. celtiformis is the closest to C. heudei with high maximum likelihood bootstrap support value. Comprehensive sampling of all Cuneopsis species is needed for phylogenetic analysis to erect new genera in future studies.

Mitogenomic phylogeny resolves Cuneopsis (Bivalvia: Unionidae) as polyphyletic: The description of two new genera and a new species

“…Although previous molecular studies have thrown some light on Cuneopsis classification, generic monophyly and inter-species relationships were weakly supported (Huang et al, 2019;Wu et al, 2018). With the expansion of taxa sampling (Lopes-Lima et al, 2020) and datasets (Dai et al, 2021;Wu et al, 2019), Cuneopsis was found to be non-monophyletic and still not well resolved with its allied genera (including Nodularia, Schistodesmus, and Inversiunio). Therefore, a conclusive assessment on Cuneopsis should include the type species C. celtiformis, together with comprehensive species sampling.…”

“…As the F-type mitogenomes of Cuneopsis species have been successively determined, mitochondrial phylogenomics preliminarily the polyphyly of Cuneopsis, even though affinities among Cuneopsis s.l. and its allied genera lacked strong nodal support (Dai et al, 2021;Wu et al, 2019). In our present study, all currently recognized species of Cuneopsis, except C. kiangsiensis, are included and subjected to whole mitogenome sequencing.…”

Revisiting the genus Pseudocuneopsis (Bivalvia, Unionidae): Morphology, mitochondrial phylogenomics, and the description of a new species

“…The genus is closely related to Cuneopsis Simpson, 1900, and Tchangsinaia Starobogatov, 1970. Cuneopsis sensu lato Simpson, 1900, which mainly includes species endemic to China (Wu et al., 2022), is polyphyletic in recent studies (Dai et al., 2021; Wu et al., 2019). Based on mitochondrial phylogenomic analyses, our previous research (Wu et al., 2022) has confirmed and categorized Cuneopsis s.l .…”