Complete Genomic Sequence of Campylobacter jejuni subsp.
            <i>jejuni</i>
            HS:19 Strain RM1285 Isolated from Packaged Chicken

Parker, Craig T.; Huynh, Steven; Heikema, Astrid P.

doi:10.1128/genomea.01100-16

Cited by 3 publications

(6 citation statements)

References 14 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These proteins include the eight enzymes known to be responsible for the Omethyl phosphoramidate decoration of the capsule [Cj1415− Cj1421 in C. jejuni NCTC 11168 (HS:2) and HS19.01− HS19.07 in C. jejuni NCTC 12517 (HS: 19)]. [9][10][11][12][13]22,49 Except for HS19.07 and Cj1421/Cj1422, the sequence identities for these proteins are ≥97%. The other relevant homologous enzymes include Cj1435 (HAD phosphatase), Cj1437 (PLPdependent aminotransferase), and Cj1441 (UDP-glucose 6dehydrogenase) where the amino acid sequence identity is >55%.…”

Section: ■ Discussionmentioning

confidence: 99%

“…The genes for these five enzymes are found in the CPS gene cluster of strain NCTC 11168, and close homologues (except for Cj1436) are found in strain NCTC 12517 (HS:19). 22 Cj1435 is predicted to be a haloacid dehalogenase (HAD) phosphatase. Cj1436 and Cj1437 are predicted to be pyridoxal phosphate (PLP)-containing enzymes.…”

mentioning

confidence: 99%

“…The enzymes most likely involved in the biosynthesis and formation of the glucuronamide moiety to the growing polysaccharide chain include Cj1435–Cj1438 and Cj1441 for HS:2. The genes for these five enzymes are found in the CPS gene cluster of strain NCTC 11168, and close homologues (except for Cj1436) are found in strain NCTC 12517 (HS:19) . Cj1435 is predicted to be a haloacid dehalogenase (HAD) phosphatase.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Functional and Structural Characterization of the UDP-Glucose Dehydrogenase Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni

Riegert

Raushel

2021

Biochemistry

View full text Add to dashboard Cite

Campylobacter jejuni is a pathogenic organism that can cause campylobacteriosis in children and adults. Most commonly, campylobacter infection is brought on by consumption of raw or undercooked poultry, unsanitary drinking water, or pet feces. Surrounding the C. jejuni bacterium is a coat of sugar molecules known as the capsular polysaccharide (CPS). The capsular polysaccharide can be very diverse among the different strains of C. jejuni, and this diversity is considered important for evading the host immune system. Modifications to the CPS of C. jejuni NCTC 11168 include O-methylation, phosphoramidylation, and amidation of glucuronate with either serinol or ethanolamine. The enzymes responsible for amidation of glucuronate are currently unknown. In this study, Cj1441, an enzyme expressed from the CPS biosynthetic gene cluster in C. jejuni NCTC 11168, was shown to catalyze the oxidation of UDP-α-d-glucose into UDP-α-d-glucuronic acid with NAD+ as the cofactor. No amide products were found in an attempt to determine whether the putative thioester intermediate formed during the oxidation of UDP-glucose by Cj1441 could be captured in the presence of added amines. The three-dimensional crystal structure of Cj1441 was determined in the presence of NAD+ and UDP-glucose bound in the active site of the enzyme (Protein Data Bank entry 7KWS). A more thorough bioinformatic analysis of the CPS gene cluster suggests that the amidation activity is localized to the t-terminal half of Cj1438, a bifunctional enzyme that is currently annotated as a sugar transferase.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Functional and Structural Characterization of the UDP-Glucose Dehydrogenase Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni

Riegert

Raushel

2021

Biochemistry

View full text Add to dashboard Cite

show abstract

“…The complete genome sequence of this Canadian strain, also known as the HS:19 Penner reference strain, was published previously [ 13 ]. The food-related strain was isolated from packed chicken meat in the USA, and its full genome was also published previously [ 14 ]. The genomes of seven C. jejuni strains with serotypes other than HS:19 and genetically diverse backgrounds, other than ST-22, were also used in this study.…”

Section: Methodsmentioning

confidence: 99%

Biomolecule sulphation and novel methylations related to Guillain-Barré syndrome-associated Campylobacter jejuni serotype HS:19

et al. 2021

Self Cite

View full text Add to dashboard Cite

Campylobacter jejuni strains that produce sialylated lipooligosaccharides (LOS) can cause the immune-mediated disease Guillain-Barré syndrome (GBS). The risk of GBS after infection with C. jejuni Penner serotype HS:19 is estimated to be at least six times higher than the average risk. Aside from LOS biosynthesis genes, genomic characteristics that promote an increased risk for GBS following C. jejuni HS:19 infection, remain uncharacterized. We hypothesized that strains with the HS:19 serotype have unique genomic features that explain the increased risk for GBS. We performed genome sequencing, alignments, single nucleotide polymorphisms' analysis and methylome characterization on a subset, and pan-genome analysis on a large number of genomes to compare HS:19 with non-HS:19 C. jejuni genome sequences. Comparison of 36 C. jejuni HS:19 with 874 C. jejuni non-HS:19 genome sequences led to the identification of three single genes and ten clusters containing contiguous genes that were significantly associated with C. jejuni HS:19. One gene cluster of seven genes, localized downstream of the capsular biosynthesis locus, was related to sulphation of biomolecules. This cluster also encoded the campylobacter sialyl transferase Cst-I. Interestingly, sulphated bacterial biomolecules such as polysaccharides can promote immune responses and, therefore, (in the presence of sialic acid) may play a role in the development of GBS. Additional gene clusters included those involved in persistence-mediated pathogenicity and gene clusters involved in restriction-modification systems. Furthermore, characterization of methylomes of two HS:19 strains exhibited novel methylation patterns (5′-CATG-3 and 5′-m6AGTNNNNNNRTTG-3) that could differentially effect gene-expression patterns of C. jejuni HS:19 strains. Our study provides novel insight into specific genetic features and possible virulence factors of C. jejuni associated with the HS:19 serotype that may explain the increased risk of GBS.

show abstract

“…jejuni strains with the capsular serotype HS:41 and strain C. jejuni subsp. jejuni RM1285 (HS:19) ( 16 , 17 ). RM3420 also possesses a C. jejuni Mu-like prophage that carries the dns gene encoding an extracellular deoxyribonuclease, previously shown to inhibit natural transformation of C. jejuni ( 18 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Genomic Sequence of Campylobacter jejuni subsp. jejuni HS:19 Penner Serotype Reference Strain RM3420

2017

Self Cite

View full text Add to dashboard Cite

show abstract

Complete Genomic Sequence of Campylobacter jejuni subsp. jejuni HS:19 Strain RM1285 Isolated from Packaged Chicken

Cited by 3 publications

References 14 publications

Functional and Structural Characterization of the UDP-Glucose Dehydrogenase Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni

Functional and Structural Characterization of the UDP-Glucose Dehydrogenase Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni

Biomolecule sulphation and novel methylations related to Guillain-Barré syndrome-associated Campylobacter jejuni serotype HS:19

Genomic Sequence of Campylobacter jejuni subsp. jejuni HS:19 Penner Serotype Reference Strain RM3420

Contact Info

Product

Resources

About