Complete genome sequence of Vibrio vulnificus FORC_017 isolated from a patient with a hemorrhagic rash after consuming raw dotted gizzard shad

Chung, Han Young; Kim, You‐Tae; Kim, Su Yeon; Na, Eun Jung; Ku, Hye‐Jin; Lee, Keun Hwa; Heo, Sang Taek; Ryu, Sangryeol; Kim, Heebal; Choi, Sang Ho; Lee, Ju‐Hoon

doi:10.1186/s13099-016-0104-6

Cited by 13 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…piscis strains sequenced to date with an identity and coverage of almost 100% and it was also in a conjugative‐virulence plasmid (pVh1) (Pajuelo et al ., 2015) harboured by one strain of V. harveyi isolated from a diseased farmed seabass (Pajuelo et al ., ). Surprisingly, both genes were also present in other recently isolated V. vulnificus strains, one from a woman infected after ingestion of raw fish (sushi) and the other from a fish farm water (Danin‐Poleg et al ., ; Chung et al ., ) (Fig. D).…”

Section: Discussionmentioning

confidence: 85%

Adaptation to host in Vibrio vulnificus, a zoonotic pathogen that causes septicemia in fish and humans

Hernández‐Cabanyero

Lee

Tolosa‐Enguis

et al. 2019

Environmental Microbiology

View full text Add to dashboard Cite

Summary Vibrio vulnificus is a siderophilic pathogen spreading due to global warming. The zoonotic strains constitute a clonal‐complex related to fish farms that are distributed worldwide. In this study, we applied a transcriptomic and single gene approach and discover that the zoonotic strains bypassed the iron requirement of the species thanks to the acquisition of two iron‐regulated outer membrane proteins (IROMPs) involved in resistance to fish innate immunity. Both proteins have been acquired by horizontal gene transfer and are contributing to the successful spreading of this clonal‐complex. We have also discovered that the zoonotic strains express a virulent phenotype in the blood of its main susceptible hosts (iron‐overloaded humans and healthy eels) by combining a host‐specific protective envelope with the common expression of two toxins (VvhA and RtxA1), one of which (RtxA1) is directly involved in sepsis. Finally, we found that both IROMPs are also present in other fish pathogenic species and have recently been transmitted to the phylogenetic lineage involved in human primary sepsis after raw seafood ingestion. Together our results highlight the potential hazard that the aquaculture industry poses to public health, which is of particular relevance in the context of a warming world.

show abstract

Section: Discussionmentioning

confidence: 85%

Adaptation to host in Vibrio vulnificus, a zoonotic pathogen that causes septicemia in fish and humans

Hernández‐Cabanyero

Lee

Tolosa‐Enguis

et al. 2019

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Despite many studies being conducted on the physiological function of CadA in V. vulnificus (VvCadA), very little is known about its enzymatic characteristics. Draft or complete genome sequences of several strains of V. vulnificus have been reported (Efimov et al, 2015; Chung et al, 2016), and these genomes generally contain one cadA , but not ldcC .…”

Section: Introductionmentioning

confidence: 99%

Biochemical Characterization and Phylogenetic Analysis of the Virulence Factor Lysine Decarboxylase From Vibrio vulnificus

Han

Yuan

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

Cadaverine is produced in organisms from the amino acid lysine in a decarboxylation reaction catalyzed by lysine decarboxylase (EC 4.1.1.18). The inducible lysine decarboxylase CadA plays a vital role in acid stress response for enteric bacteria. Vibrio vulnificus is an extremely virulent human pathogen causing gastroenteritis when the acid conditions that prevent survival of V. vulnificus in the stomach or small intestine are overcome. A gene encoding CadA was identified from V. vulnificus. Subsequent analyses showed that CadA from V. vulnificus (VvCadA) is a decamer with a 82-kDa subunit. Homogenous VvCadA was purified from Escherichia coli and used for lysine decarboxylation with an optimal pH of 6.0 and optimal temperature of 37°C. The apparent Vmax and Km for lysine were 9.45 ± 0.24 μM/min and 0.45 ± 0.05 mM, respectively. Mutation analysis suggested that the amino-acid-binding pyridoxal phosphate, the cofactor of the enzyme, plays a vital role in the reaction. Mutation of the negatively charged residues interacting with lysine also affected the activity of the enzyme to some extent. Quantitative RT-PCR showed that expression of VvcadA was up-regulated under low pH, low salinity, and oxidative stresses. Furthermore, the concentration of cadaverine released to the cell exterior also increased under these stresses. Protein sequence similarity network (SSN) analysis indicated that lysine decarboxylases with ornithine decarboxylases and arginine decarboxylases shared a common ancestor, and that lysine decarboxylases are more conserved during evolution. Our data provide evidence for the biochemical characteristics and important roles of VvCadA under stress conditions.

show abstract

“…vulnificus reference genomes. The estimated sizes for the entire CICESE-316 and CICESE-325 genomes were ~4.76 and ~4.71 Mb, respectively—smaller than the 5.2 Mb that has been reported for CMCP6 and FORC_017, the 5.3-Mb YJ016 genome, and the 5.0-Mb MO6-24/O genome [26, 27, 41]. These data show that the differences between C-genotype V .…”

Section: Discussionmentioning

confidence: 64%

Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

et al. 2019

View full text Add to dashboard Cite

Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V . vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE , the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection.

show abstract

Complete genome sequence of Vibrio vulnificus FORC_017 isolated from a patient with a hemorrhagic rash after consuming raw dotted gizzard shad

Cited by 13 publications

References 23 publications

Adaptation to host in Vibrio vulnificus, a zoonotic pathogen that causes septicemia in fish and humans

Adaptation to host in Vibrio vulnificus, a zoonotic pathogen that causes septicemia in fish and humans

Biochemical Characterization and Phylogenetic Analysis of the Virulence Factor Lysine Decarboxylase From Vibrio vulnificus

Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

Contact Info

Product

Resources

About