Complete Genome Sequence of the First Aichi Virus Isolated in Taiwan

Chang, Jenn-Tzong; Chen, Yao-Shen; Chen, Bao-Chen; Chao, David; Chang, Tsung-Hsien

doi:10.1128/genomea.00107-12

Cited by 15 publications

(14 citation statements)

References 7 publications

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“…A nucleotide difference of ≈20% from the available sequences of genotypes A, B, and C was observed, tentatively leading to the conclusion that this sequence might belong to a new genotype of Aichi virus. More sequence information is needed to substantiate this finding by isolating this Aichi virus strain and subsequently subjecting the virus to whole-genome sequencing, as was described for an Aichi virus isolated in Taiwan in 2010 ( 36 ). For 3 of the sewage water concentrates from our study, 3C sequences were detected that showed high similarities with the recently discovered canine kobuvirus ( 37 , 38 ) (Figure 4).…”

Section: Discussionmentioning

confidence: 99%

Aichi Virus in Sewage and Surface Water, the Netherlands

Lodder¹,

Rutjes²,

Takumi³

et al. 2013

Emerg. Infect. Dis.

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Detection of Aichi virus in humans was initially reported in Japan in 1989. To establish a timeline for the prevalence of Aichi virus infection among humans in the Netherlands, we conducted molecular analysis of archival water samples from 1987–2000 and 2009–2012. Aichi virus RNA was detected in 100% (8/8) of sewage samples and 100% (7/7) of surface water samples collected during 1987–2000 and 100% (8/8) of sewage samples and 71% (5/7) of surface water samples collected during 2009–2012. Several genotype A and B Aichi virus lineages were observed over the 25-year period studied, but the time course of viral genetic diversity showed recent expansion of the genotype B population over genotype A. Our results show that Aichi virus has been circulating among the human population in the Netherlands since before its initial detection in humans was reported and that genotype B now predominates in this country.

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Section: Discussionmentioning

confidence: 99%

Aichi Virus in Sewage and Surface Water, the Netherlands

Lodder¹,

Rutjes²,

Takumi³

et al. 2013

Emerg. Infect. Dis.

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“…Among those, two complete genome sequences of T. phaeum [ 9 ], “T. acetatoxydans” [ 10 ] and one draft genome sequence of C. ultunense [ 11 ] have been published, the later two by this working group. Here, we are presenting the draft genome sequence of the third mesophilic SAOB Syntrophaceticus schinkii strain Sp3.…”

Section: Introductionmentioning

confidence: 99%

Working draft genome sequence of the mesophilic acetate oxidizing bacterium Syntrophaceticus schinkii strain Sp3

Manzoor

Müller

Niazi

et al. 2015

Stand in Genomic Sci

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Syntrophaceticus schinkii strain Sp3 is a mesophilic syntrophic acetate oxidizing bacterium, belonging to the Clostridia class within the phylum Firmicutes, originally isolated from a mesophilic methanogenic digester. It has been shown to oxidize acetate in co-cultivation with hydrogenotrophic methanogens forming methane. The draft genome shows a total size of 3,196,921 bp, encoding 3,688 open reading frames, which includes 3,445 predicted protein-encoding genes and 55 RNA genes. Here, we are presenting assembly and annotation features as well as basic genomic properties of the type strain Sp3.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0092-z) contains supplementary material, which is available to authorized users.

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“…The human AiV, genome accession no. JX56424 25 , was isolated from a newborn with diarrhea in Taiwan and propagated in Vero cells (ATCC: CCL-81, Manassas, VA, USA). Human lung carcinoma A549 cells (ATCC: CCL-185) and HEK293T cells (ATCC: CRL-3216) were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 100 μg/ml penicillin/streptomycin (Gibco, ThermoFisher Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Aichi virus 3C protease modulates LC3- and SQSTM1/p62-involved antiviral response

2020

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Rationale: Autophagy is an essential, homeostatic process by which cells break down their own components, it also contributes to restricting bacterial infection in host defense systems; yet, how autophagy regulates viral infection remains inconclusive. Aichi virus (AiV), belonging to the genus Kobuvirus in the Picornaviridae family, causes acute gastroenteritis in human. The role of autophagy-mediated anti-viral activity on AiV infection was investigated in this study. Methods: The effect of autophagy-associated molecules in retinoic acid-inducible gene-I (RIG-I)-like receptor (RLR) antiviral signal axis was analyzed in AiV infected cells by using biochemistry and pharmacologic approaches. In addition, the AiV viral protein regulating autophagy-associated RLR activity was also evaluated. Results : In AiV-infected cells, autophagic flux including the formation of autophagic vacuoles, as well as degradation of microtubule-associated protein light chain 3 (LC3) and sequestosome-1 (SQSTM1/p62) were observed. Ectopic overexpression of LC3 and p62, but not Atg proteins, contributed to RLR antiviral signal axis, shRNA knockdown of LC3 and p62 led to a downregulation of antiviral inflammation. Moreover, AiV infection inhibited double-stranded RNA (dsRNA)-activated RLR activity by the viral protein 3C protease but not H42D, C143S protease dead mutants. AiV 3C protease caused the degradation of LC3 and p62, and also RLR signal proteins. Conclusion: This study reveals a possible mechanism of autophagy-associated proteins regulating virus replication. Maintaining a cellular level of LC3 and p62 during the viral infection period might help restrict virus replication. Although, AiV 3C protease dampens the LC3 and p62-mediated host antiviral machinery for AiV replication. Results obtained provide a better understanding of the molecular pathogenesis of AiV for developing methods of prevention and treatment.

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Complete Genome Sequence of the First Aichi Virus Isolated in Taiwan

Cited by 15 publications

References 7 publications

Aichi Virus in Sewage and Surface Water, the Netherlands

Aichi Virus in Sewage and Surface Water, the Netherlands

Working draft genome sequence of the mesophilic acetate oxidizing bacterium Syntrophaceticus schinkii strain Sp3

Aichi virus 3C protease modulates LC3- and SQSTM1/p62-involved antiviral response

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