Complete Genome Sequence of Pseudomonas fluorescens LBUM636, a Strain with Biocontrol Capabilities against Late Blight of Potato

Morrison, Christopher K.; Novinscak, Amy; Gadkar, Vijay; Joly, David L.; Filion, Martin

doi:10.1128/genomea.00446-16

Cited by 12 publications

(6 citation statements)

References 6 publications

(8 reference statements)

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“…Very few culturable strains of P. yamanorum are known (8H1 (Arnau et al, 2015), LP2 (Komesli et al, 2020), LBUM636 (Morrison et al, 2016)), and P. arsenicoxi dans (VC-1 (Campos et al, 2010)), however, only P. arsenicoxydans ACM1 (GenBank: ASM413599v1) was isolated from the moss rhizosphere in Antarctica.…”

Section: Discussionmentioning

confidence: 99%

Bacteria of the genus Pseudomonas isolated from Antarctic substrates

Hnatush

Komplikevych

Maslovska

et al. 2021

UAJ

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The study’s primary purposes were establishing the number of microorganisms that exhibit hydrolytic activity in Antarctic soil and mosses samples, isolation of metal-resistant strains of bacteria, and description of their physiological and biochemical properties. Samples collected during the XXIII Ukrainian Antarctic Expedition in 2019 were used. The number of colony-forming units of microorganisms exhibiting proteolytic, amylolytic, cellulase, lipolytic activity was studied. Pure bacterial cultures were isolated using standard microbiological methods. Determination of resistance of isolates to heavy metals was estimated after their cultivation during ten days on agar plates with different concentrations of CdCl2 ⋅ 2.5H2O, CoCl2 ⋅ 6H2O, K2Cr2O7, FeSO4 ⋅ 7H2O, CuCl2 ⋅ 2H2O. Identification of strains was based on the sequencing of the 16S rRNA gene, morphological, physiological, and biochemical properties. Among the 23 isolates, nine metal-resistant strains were selected, four of which were identified as Pseudomonas yamanorum ІМV B-7916 and 79_102, and as P. arsenicoxidans 5A_1N_24, and 89_1T_89. Among the selected strains, the most resistant to heavy metals was P. yamanorum 79_102. All studied strains synthesize lipases during growth on medium with tween-20, which contains 0.5–1 mM of ferrous sulfate and copper (II) chloride. The studied strains produce exopolysaccharides during growth at 6 and 22 °C. The most effective among these strains exopolysaccharides are synthesized by P. arsenicoxidans 5A_1N_24 — 768 mg/g of dry weight. Our results expand the knowledge about the diversity of microorganisms of extreme biotopes, their properties, resistance to heavy metal compounds.

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Section: Discussionmentioning

confidence: 99%

Bacteria of the genus Pseudomonas isolated from Antarctic substrates

Hnatush

Komplikevych

Maslovska

et al. 2021

UAJ

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“…The phenazines are small molecules that can penetrate different types of cells, including prokaryotes. Many phenazines present antimicrobial activity, suppressing pathogenic microorganisms of humans, animals and plants, also playing an important role in the survival strategies of bacteria in the environment (Chen et al, 2014 ; Briard et al, 2015 ; Dasgupta et al, 2015 ; Garrison et al, 2016 ; Morrison et al, 2016 ). The P. aeruginosa LV strain is a great producer of phenazine carboxylic acid (PCA).…”

Section: Discussionmentioning

confidence: 99%

New Insights about Antibiotic Production by Pseudomonas aeruginosa: A Gene Expression Analysis

et al. 2017

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The bacterial resistance for antibiotics is one of the most important problems in public health and only a small number of new products are in development. Antagonistic microorganisms from soil are a promising source of new candidate molecules. Products of secondary metabolism confer adaptive advantages for their producer, in the competition for nutrients in the microbial community. The biosynthesis process of compounds with antibiotic activity is the key to optimize their production and the transcriptomic study of microorganisms is of great benefit for the discovery of these metabolic pathways. Pseudomonas aeruginosa LV strain growing in the presence of copper chloride produces a bioactive organometallic compound, which has a potent antimicrobial activity against various microorganisms. The objective of this study was to verify overexpressed genes and evaluate their relation to the organometallic biosynthesis in this microorganism. P. aeruginosa LV strain was cultured in presence and absence of copper chloride. Two methods were used for transcriptomic analysis, genome reference-guided assembly and de novo assembly. The genome referenced analysis identified nine upregulated genes when bacteria were exposed to copper chloride, while the De Novo Assembly identified 12 upregulated genes. Nineteen genes can be related to an increased microbial metabolism for the extrusion process of exceeding intracellular copper. Two important genes are related to the biosynthesis of phenazine and tetrapyrroles compounds, which can be involved in the bioremediation of intracellular copper and we suggesting that may involve in the biosynthesis of the organometallic compound. Additional studies are being carried out to further prove the function of the described genes and relate them to the biosynthetic pathway of the organometallic compound.

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“…In 2016, the complete genome of Pseudomonas fluorescens LBUM636, a strain with significant biocontrol activity against Phytophthora infestans , was sequenced, providing a bioinformatic reference for mining biocontrol mechanisms 54 . Subsequently, it was reported that phenazine‐1‐carboxylic acid (PCA), produced by Pseudomonas fluorescens strain LBUM636, probably plays a key role in the biocontrol activity of this strain against Phytophthora infestans , which was revealed through comparisons between the wild‐type strain and its isogenic mutant phzC‐ that does not produce PCA 55 .…”

Section: Bacteria As Biocontrol Agents and Their Modes Of Actionmentioning

confidence: 99%

A review of biocontrol agents in controlling late blight of potatoes and tomatoes caused by Phytophthora infestans and the underlying mechanisms

Wang

Long

2023

Pest Management Science

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Phytophthora infestans causes light blight on potatoes and tomatoes, which has a significant economic impact on agriculture. The management of late blight has been largely dependent on the application of synthetic fungicides, which is not ultimate solution for sustainable agriculture and environmental safety. Biocontrol strategies are expected to be alternative methods to the conventional chemicals in controlling plant diseases in the integrated pest management (IPM) programs. Well‐studied biocontrol agents against P. infestans include fungi, oomycetes, bacteria, and compounds produced by these antagonists, in addition to certain bioactive metabolites produced by plants. Laboratory and greenhouse experiments suggest a potential for using biocontrol in practical late blight disease management. However, the transition of biocontrol to field applications is problematic for the moment, due to low and variable efficacies. In this review, we provide a comprehensive summary on these biocontrol strategies and the underlying corresponding mechanisms. To give a more intuitive understanding of the promising biocontrol agents against P. infestans in agricultural systems, we discuss the utilizations, modes of action and future potentials of these antagonists based on their taxonomic classifications. To achieve a goal of best possible results produced by biocontrol agents, it is suggested to work on field trials, strain modifications, formulations, regulations, and optimizations of application. Combined biocontrol agents having different modes of action or biological adaptation traits may be used to strengthen the biocontrol efficacy. More importantly, biological control agents should be applied in the coordination of other existing and forthcoming methods in the IPM programs.This article is protected by copyright. All rights reserved.

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Complete Genome Sequence of Pseudomonas fluorescens LBUM636, a Strain with Biocontrol Capabilities against Late Blight of Potato

Cited by 12 publications

References 6 publications

Bacteria of the genus Pseudomonas isolated from Antarctic substrates

Bacteria of the genus Pseudomonas isolated from Antarctic substrates

New Insights about Antibiotic Production by Pseudomonas aeruginosa: A Gene Expression Analysis

A review of biocontrol agents in controlling late blight of potatoes and tomatoes caused by Phytophthora infestans and the underlying mechanisms

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