Complete Genome Sequence of Lumpy Skin Disease Virus Isolate SERBIA/Bujanovac/2016, Detected during an Outbreak in the Balkan Area

Toplak, Ivan; Petrović, Tamaš; Vidanović, Dejan; Lazić, Sava; Šekler, Milanko; Manić, Marija; Petrović, Milun; Kuhar, Urška

doi:10.1128/genomea.00882-17

Cited by 22 publications

(16 citation statements)

References 8 publications

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“…The modifi cation of VNT in relation to the recommended VNT by OIE [12] refers to the virus used, cell culture and duration of the test. VNT was performed using homologous virus SERBIA/Bujanovac/2016 that originated from LSD clinically infected animal [10,11], and was isolated on MDBK cell line. On the other hand, OIE recommends using Capripoxvirus for VNT [12].…”

Section: Discussionmentioning

confidence: 99%

“…The isolated LSDV named SERBIA/Bujanovac/2016 was sequenced in the length of the complete genome and submitted into NCBI GenBank under the number KY702007. Sequencing of the genome revealed that LSDV isolated in Serbia consists of 150661 nucleotides and that it shares 99.95% of nucleotide identity with the Neethling Warmbaths LW strain, which was isolated in South Africa in 1999 [11].…”

Section: Introductionmentioning

confidence: 99%

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Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods

et al. 2019

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Infection of cattle with lumpy skin disease virus (LSDV) is very important from the aspect of livestock production. Although it can cause significant economic losses, available serological assays are still not sufficiently efficient and reliable. A 3-day VNT was performed using Madin-Darby bovine kidney (MDBK) cell line and LSDV isolated from clinically infected cow to improve serological diagnostics of lumpy skin disease (LSD). In total, 325 cattle sera samples were tested in order to compare the performances of VNT and ELISA. Tested samples originated from 125 cows before the occurrence of LSD in the Republic of Serbia and 200 tested samples originated from vaccinated cows. Sera samples from vaccinated cows were collected starting from the vaccination day to 4 months after vaccination. In 7 different time intervals after vaccination sampling was carried out in 20 cows originating from one herd and in 3 different time intervals in 20 cows originating from a different herd each time of sampling. Out of 200 samples from vaccinated cows, antibodies against LSDV were detected in 68 (34%) samples by VNT, and in 60 (30%) samples by ELISA. No positive finding was detected by VNT in samples collected before the occurrence of LSD in Serbia, while one positive finding was detected in the same samples by ELISA. The first presence of antibodies in vaccinated cattle was detected by both tests 20 days after vaccination, and the largest number of animals with antibodies against LSDV was detected 30 days after vaccination. Comparing the results obtained by VNT and ELISA, it was calculated that kappa index was 0.913. The specificity of VNT and ELISA was 100% and 99.2%, respectively. VNT is simpler to perform compared to the recommended virus neutralization test by the OIE and can improve LSD serological diagnostics with additional sensitivity testing.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods

et al. 2019

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“…Phylogenetic analysis indicated that the LSDV strain Serbia/Bujanovac/2016 had higher sequence homology with other field LSDV strains (>99.8%) than to vaccine strains (>98.8%). The highest sequence homology of 99.99% was found with field strain Evros/GR/15 (Toplak et al, ). Phylogenetic analysis of whole genome sequences provides more detailed information about genetic differences between the LSDV strains than the phylogenetic analysis based on sequences of only one gene.…”

Section: Discussionmentioning

confidence: 99%

“…The isolated LSDV was completely sequenced (NCBI GenBank, number ) (Toplak et al, ) and compared to available complete genome sequences of field and vaccine lumpy skin disease viruses (LSDV), sheep pox viruses (SPPV) and goat pox viruses (GTPV) obtained from GenBank (http://www.ncbi.nlm.nih.gov). The evolutionary history was inferred using the Neighbour‐Joining method.…”

Section: Methodsmentioning

confidence: 99%

Epizootic features and control measures for lumpy skin disease in south‐east Serbia in 2016

Manić¹,

Stojiljković²,

Petrović³

et al. 2019

Transbound Emerg Dis

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Lumpy skin disease (LSD) is an infectious disease of cattle caused by virus of the Capripoxvirus genus (LSDV), family Poxviridae. Until 2015, it had not appeared in the Balkans. In June 2016, LSD spread throughout Serbia. This study analyses the first LSD occurrence, epizootic features, applied diagnostic procedures and control measures in five districts in south‐east Serbia (Pcinja, Jablanica, Pirot, Toplica and Nisava). In total, there were 225 LSD outbreaks reported in Serbia, out of which 189 (84%) were located in the study area. The highest number of outbreaks was registered in Pcinja district (169), where LSD was first registered. The median distance and time between the nearest previous outbreak sites were calculated (4.32 km and 9 days). The median altitude of outbreak locations was 992 m with more than 90% above 500 m (p ≤ 0.001). The average herd morbidity rate in the study area was 13.6% and the herd mortality rate was recorded only in Pcinja (0.5%) and Jablanica (1.6%) districts. Samples taken from the cattle suspected to LSD were subjected to real time PCR analysis. Out of 233 samples tested for LSDV 132 (56.7%) were positive. The LSDV genome was identified in skin nodules (85.4%), blood (72.7%) and nasal swabs (62.5%). Phylogenetic analysis indicated that the LSDV strain circulating in Serbia fell within the cluster of field LSDV found worldwide. In response to the LSD epizootic, animal trade and movement were prohibited, complete stamping out, disinfection, disinsection and an entire bovine population vaccination using the homologous Neethling live attenuated vaccine (OBP, South Africa) were conducted. A month and a half after the completion of the vaccination campaign, the LSD epizootic was stopped, and no new cases have been reported since.

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“…In mid-2016, there were reports on the spread of this highly dangerous infectious disease in the Republic of Serbia, which were followed by amendments to the Ordinance on the 2016 Animal Health Protection Program (OGRS, 2016). Emergency vaccination was carried out primarily in Pčinja and Jablanica Districts using the vaccine donated by the European Commission as an aid to Serbia to control and prevent the spread of LSD (OGRS, 2016;Toplak et al, 2017), and later according to the plan in other districts. Apart from the health aspect, the disease has a significant economic impact due to animal production losses (reduced milk production, reduced quality of the skin), restrictions on the movement of susceptible animals and restrictive trade measures which are in force in infected areas (VAMA, 2016a).…”

Section: Introductionmentioning

confidence: 99%

Total Number of Cattle in the Republic of Serbia after an Outbreak of Lumpy Skin Disease

Lazić¹,

Nikitović

Antić

et al. 2019

Agro-know. J.

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Numerous studies conducted worldwide indicate a tendency towards a continuous decline in number of cattle. Appropriate measures should be taken to prevent the reduction of the number of cattle and to increase the concentration of animals per farm. In this regard, the objective of this study was to analyze the total number of cattle after an outbreak of Lumpy Skin Disease (LSD) in the Republic of Serbia. Due to the occurrence of this highly dangerous infectious disease in the Balkans and Eastern Europe, emergency and preventive vaccination was performed in infected and vulnerable areas in the Republic of Serbia. To prevent the spread of LSD, competent authorities banned public gatherings of cattle at fairs, exhibitions, livestock markets and purchase places, which resulted in economic losses for certain farmers. This disease is believed to have impacted the total number of cattle slaughtered and the number of deaths, as well as the import and export of live cattle, animal products and by-products of animal origin. The comparison between indices for the number of cattle shows a decline in the total number of cattle in the analyzed period across statistical territorial units. Results on the total number of cattle can be used, among other things, to create new and more complex questions arising from current and future farming problems.

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Complete Genome Sequence of Lumpy Skin Disease Virus Isolate SERBIA/Bujanovac/2016, Detected during an Outbreak in the Balkan Area

Cited by 22 publications

References 8 publications

Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods

Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods

Epizootic features and control measures for lumpy skin disease in south‐east Serbia in 2016

Total Number of Cattle in the Republic of Serbia after an Outbreak of Lumpy Skin Disease

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