Complete Genome Sequence of
            <i>Escherichia</i>
            Phage Lw1, a New Member of the RB43 Group of Pseudo T-Even Bacteriophages

Kushkina, Alla; Товкач, Ф. И.; Comeau, A.; Kostetskii, Igor; Lisovski, Igor; Остапчук, А. М.; Voychuk, S.I.; Gorb, Tetiana; Romaniuk, Liudmyla

doi:10.1128/genomea.00743-13

Cited by 6 publications

(10 citation statements)

References 7 publications

(8 reference statements)

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“…Like RB43, Miller encodes a single tRNA gene. In RB43, the tRNA is an Ile where as in Miller, it is a Met as has been reported in RB43-like Escherichia coli phage Lw1 ( 7 ).…”

Section: Genome Announcementsupporting

confidence: 52%

“…Unlike RB43, which has a fused rIIa and rIIb gene, Miller has separate rIIa and rIIb genes that share an overlapping start/stop codon. As with RB43 and other pseudo T-even phages, the genome of Miller is opened to rIIb ( 7 ). Miller has a 178,171-base pair genome, a coding density of 95.6%, 43.13% G+C content, and 277 predicted genes.…”

Section: Genome Announcementmentioning

confidence: 99%

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Complete Genome Sequence of Citrobacter freundii Myophage Miller

et al. 2015

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“…Like RB43, Miller encodes a single tRNA gene. In RB43, the tRNA is an Ile where as in Miller, it is a Met as has been reported in RB43-like Escherichia coli phage Lw1 ( 7 ).…”

Section: Genome Announcementsupporting

confidence: 52%

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Citrobacter freundii Myophage Miller

et al. 2015

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“…Phage DL64 have a large genome compared with the other two, and DL54 showed the highest gene density. Interestingly, phage DL64 appears to carry the rIIA/rIIB lysis inhibition cassette, which is widely distributed among the genomes of phages infecting different host genera (Kushkina et al ., ; Wittmann et al ., ). This enables a delay in host lysis, but consequently results in a high increase in the burst size of the phage, providing phages of this type with a competitive advantage over others.…”

Section: Discussionmentioning

confidence: 99%

A novel bacteriophage cocktail reduces and disperses Pseudomonas aeruginosa biofilms under static and flow conditions

Alves

Esteban

Kot

et al. 2015

Microbial Biotechnology

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Summary P seudomonas aeruginosa is an opportunistic human pathogen that forms highly stable communities – biofilms, which contribute to the establishment and maintenance of infections. The biofilm state and intrinsic/acquired bacterial resistance mechanisms contribute to resistance/tolerance to antibiotics that is frequently observed in P . aeruginosa isolates. Here we describe the isolation and characterization of six novel lytic bacteriophages: viruses that infect bacteria, which together efficiently infect and kill a wide range of P . aeruginosa clinical isolates. The phages were used to formulate a cocktail with the potential to eliminate P . aeruginosa PAO1 planktonic cultures. Two biofilm models were studied, one static and one dynamic, and the phage cocktail was assessed for its ability to reduce and disperse the biofilm biomass. For the static model, after 4 h of contact with the phage suspension (MOI 10) more than 95% of biofilm biomass was eliminated. In the flow biofilm model, a slower rate of activity by the phage was observed, but 48 h after addition of the phage cocktail the biofilm was dispersed, with most cells eliminated (> 4 logs) comparing with the control. This cocktail has the potential for development as a therapeutic to control P . aeruginosa infections, which are predominantly biofilm centred.

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“…Despite the fact, that peak III fractions contained a fairly large number of siphovirions with filled heads (appeared as dark capsids on EM-micrograph, Fig 3, b), a comparative restriction analysis of total phage DNA revealed no difference in the corresponding patterns (Fig 4). The patterns appeared to be identical to those of phage Lw1, which had been isolated from this primary phage pool earlier [3]. To disprove a speculation that virion nucleic acid of the siphophages was destroyed during manipulations, TAFE-separation of total phage DNA from the same samples was conducted.…”

Section: Restriction Patterns Of the Chromatographic Peaksmentioning

confidence: 73%

“…Beckman SW50 rotor (100 000 g, 4 °C, 4h) for additional phage purification and separation. The gradient steps had densities of 1.4, 1.5 and 1.6 g/cm 3 [8]. From the other side, dialysis of salt-containing phage samples leads to their diluting and, thus, decreasing of phage particle concentration.…”

Section: Cscl-gradient Centrifugation Of the Peak Fractions Was Perfomentioning

confidence: 99%

Biodiversity Of Bacteriophages From Lysed Batch Culture Of RecombinantEscherichia ColiBL21(DE3) Examined By Anion Exchange Chromatography And TEM

Kushkina

Товкач

2020

Preprint

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It was found that the phage population assaulted a batch culture of an industrial recombinant derivative of E. coli BL21(DE3) was attenuated that manifested in producing pinprick-type plaques and their inability to propagation in subsequent passages. Because of this, the goals of the present research were to evaluate biodiversity and scrutinize the possible virion structural defects of the attenuated phage population prior to pure phage lines isolation. The anion exchange chromatography (AEC) was chosen as the principal method allowing to get a comparative phage population profile based on the virion net surface charge, as well as to treat the 5-liter virion-contained sample and collect high-quality concentrated and separated phage fractions to further analysis. The isolate consisted of a mix of two phages belonging to Myoviridae (A2-morphotype) and Siphoviridae (B1morphotype) families. By restriction analysis, the main portion of this phage pool (about 99% of all virions) was identified as the primary population of myophage Lw1, which possessed its own intra-population biodiversity (heterogeneity). It consisted of a major and two minor subpopulations that differed by phage capsid size and shape. The subpopulation III consisted of aberrant tubby-phages with triprolate (expanded at both sides) capsids having low aspect ratio.

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Complete Genome Sequence of Escherichia Phage Lw1, a New Member of the RB43 Group of Pseudo T-Even Bacteriophages

Cited by 6 publications

References 7 publications

Complete Genome Sequence of Citrobacter freundii Myophage Miller

Complete Genome Sequence of Citrobacter freundii Myophage Miller

A novel bacteriophage cocktail reduces and disperses Pseudomonas aeruginosa biofilms under static and flow conditions

Biodiversity Of Bacteriophages From Lysed Batch Culture Of RecombinantEscherichia ColiBL21(DE3) Examined By Anion Exchange Chromatography And TEM

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