Complete genome sequence of Gluconacetobacter xylinus E25 strain—Valuable and effective producer of bacterial nanocellulose

Kubiak, Katarzyna; Kurzawa, Marta; Jędrzejczak-Krzepkowska, Marzena; Ludwicka, Karolina; Krawczyk, Mariusz; Migdalski, Andrzej; Kacprzak, Magdalena M.; Loska, Damian; Krystynowicz, A.; Bielecki, Stanisław

doi:10.1016/j.jbiotec.2014.02.006

Cited by 51 publications

(32 citation statements)

References 19 publications

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“…Kubiak et al [36] investigated the complete genome sequence of G. xylinus E25 and identified a megaplasmid that had not been reported before. Megaplasmids have been found to be important for the survival of other Alphaproteobacteria genera in unfavorable environments.…”

Section: Discussionmentioning

confidence: 99%

“…Megaplasmids have been found to be important for the survival of other Alphaproteobacteria genera in unfavorable environments. Kubiak et al [36] also found some G. xylinus E25 genes connected to oxidoreductases, for instance H845_1089 and H845_1144. The activity of enzymes coded for by such genes may help to explain the ability of G. xylinus to grow in medium containing aromatic compounds.…”

Section: Discussionmentioning

confidence: 99%

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Effects of aromatic compounds on the production of bacterial nanocellulose by Gluconacetobacter xylinus

et al. 2014

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BackgroundBacterial cellulose (BC) is a polymeric nanostructured fibrillar network produced by certain microorganisms, principally Gluconacetobacter xylinus. BC has a great potential of application in many fields. Lignocellulosic biomass has been investigated as a cost-effective feedstock for BC production through pretreatment and hydrolysis. It is well known that detoxification of lignocellulosic hydrolysates may be required to achieve efficient production of BC. Recent results suggest that phenolic compounds contribute to the inhibition of G. xylinus. However, very little is known about the effect on G. xylinus of specific lignocellulose-derived inhibitors. In this study, the inhibitory effects of four phenolic model compounds (coniferyl aldehyde, ferulic acid, vanillin and 4-hydroxybenzoic acid) on the growth of G. xylinus, the pH of the culture medium, and the production of BC were investigated in detail. The stability of the phenolics in the bacterial cultures was investigated and the main bioconversion products were identified and quantified.ResultsConiferyl aldehyde was the most potent inhibitor, followed by vanillin, ferulic acid, and 4-hydroxybenzoic acid. There was no BC produced even with coniferyl aldehyde concentrations as low as 2 mM. Vanillin displayed a negative effect on the bacteria and when the vanillin concentration was raised to 2.5 mM the volumetric yield of BC decreased to ~40% of that obtained in control medium without inhibitors. The phenolic acids, ferulic acid and 4-hydroxybenzoic acid, showed almost no toxic effects when less than 2.5 mM. The bacterial cultures oxidized coniferyl aldehyde to ferulic acid with a yield of up to 81%. Vanillin was reduced to vanillyl alcohol with a yield of up to 80%.ConclusionsThis is the first investigation of the effect of specific phenolics on the production of BC by G. xylinus, and is also the first demonstration of the ability of G. xylinus to convert phenolic compounds. This study gives a better understanding of how phenolic compounds and G. xylinus cultures are affected by each other. Investigations in this area are useful for elucidating the mechanism behind inhibition of G. xylinus in lignocellulosic hydrolysates and for understanding how production of BC using lignocellulosic feedstocks can be performed in an efficient way.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of aromatic compounds on the production of bacterial nanocellulose by Gluconacetobacter xylinus

et al. 2014

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“…In addition, BC can be modified to enhance the performance of materials and to satisfy the requirements of certain fields . Generally, BC is generated by some special bacteria, and Gluconacetobacter xylinus is the most typical one whose complete genome sequence has been learned …”

Section: Introductionmentioning

confidence: 99%

Comparison of bacterial cellulose production by Gluconacetobacter xylinus on bagasse acid and enzymatic hydrolysates

Luo

Huang

et al. 2017

J of Applied Polymer Sci

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To fulfill the comprehensive utilization of cellulose and hemicellulose components in bagasse for bacterial cellulose (BC) production, both bagasse acid and enzymatic hydrolysates were used for BC production by Gluconacetobacter xylinus. Although the BC accumulation rate was slower during the early period of fermentation in the bagasse acid hydrolysate than in the enzymatic hydrolysate, the highest BC yield (1.09 vs. 0.42 g/L) was higher in the bagasse acid hydrolysate. The substrate utilization was evaluated in both bagasse acid and enzymatic hydrolysates, and glucose, xylose, and acetic acid were better carbon sources than arabinose and cellobiose for G. xylinus. The structure of the BC samples obtained from bagasse acid and enzymatic hydrolysates, including the microscopic morphology, functional groups, and crystals, was similar especially in the later phase of fermentation, which was analyzed by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, and X‐ray diffraction. Thus, both bagasse acid and enzymatic hydrolysates could be promising substrates for BC production. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45066.

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“…The idea of BC as a potential leather substitute rests on the industrial production of cellulose fibres by members of the genera Komagataeibacter (bacteria ingested as part of kombucha tea and other fermentations, and which enjoy Generally Regarded As Safe [GRAS] status), e.g., by the model strain K. xylinum (Kubiak et al, 2014). These microorganisms, either alone or in combination with other bacteria and yeasts, aerobically produce pellicles of cellulose that accumulate in the extracellular medium.…”

Section: Bacterial Cellulose As a Potential Leather Substitutementioning

confidence: 99%

Bacterial cellulose as a potential bioleather substitute for the footwear industry

García¹,

Prieto

2018

Microbial Biotechnology

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Complete genome sequence of Gluconacetobacter xylinus E25 strain—Valuable and effective producer of bacterial nanocellulose

Cited by 51 publications

References 19 publications

Effects of aromatic compounds on the production of bacterial nanocellulose by Gluconacetobacter xylinus

Effects of aromatic compounds on the production of bacterial nanocellulose by Gluconacetobacter xylinus

Comparison of bacterial cellulose production by Gluconacetobacter xylinus on bagasse acid and enzymatic hydrolysates

Bacterial cellulose as a potential bioleather substitute for the footwear industry

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