Complete Genome Sequence of Geobacillus thermoglucosidans TNO-09.020, a Thermophilic Sporeformer Associated with a Dairy-Processing Environment

Zhao, Yu; Caspers, Martien P. M.; Abee, Tjakko; Siezen, Roland J.; Kort, Remco

doi:10.1128/jb.00318-12

Cited by 35 publications

(24 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…JF8 (3.49 Mbp, 52.0% GC, 3584 CDS) (Shintani et al, 2014), G. thermoleovorans (3.60 Mbp, 52.3% GC, 3887 CDS) (Muhd Sakaff et al, 2012), G. kaustophilus (3.54 Mbp, 52.1% GC, 3498 CDS) (Takami et al, 2004), G. stearothermophilus (3.27 Mbp, 52.6% GC, 3042 CDS) (Rozanov et al, 2014), and to a lesser extent G. thermodenitrificans (3.55 Mbp, 49.0% GC, 3444 CDS) (Feng et al, 2007) and G. thermoglucosidasius (3.75 Mbp, 43.9% GC, 3443 CDS) (Zhao et al, 2012). The pGt3 plasmid of Geobacillus LC300 shows similarity with plasmids pHTA426 from G. kaustophilus (97% identity, 37% coverage, BLASTN), pBt40 from Geobacillus sp.…”

Section: Resultsmentioning

confidence: 99%

Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium

Cordova

Long

Venkataramanan

et al. 2015

Metabolic Engineering

View full text Add to dashboard Cite

We have isolated a new extremely thermophilic fast-growing Geobacillus strain that can efficiently utilize xylose, glucose, mannose and galactose for cell growth. When grown aerobically at 72 °C, Geobacillus LC300 has a growth rate of 2.15 h−1 on glucose and 1.52 h−1 on xylose (doubling time less than 30 minutes). The corresponding specific glucose and xylose utilization rates are 5.55 g/g/h and 5.24 g/g/h, respectively. As such, Geobacillus LC300 grows 3-times faster than E. coli on glucose and xylose, and has a specific xylose utilization rate that is 3-times higher than the best metabolically engineered organism to date. To gain more insight into the metabolism of Geobacillus LC300 its genome was sequenced using PacBio’s RS II single-molecule real-time (SMRT) sequencing platform and annotated using the RAST server. Based on the genome annotation and the measured biomass composition a core metabolic network model was constructed. To further demonstrate the biotechnological potential of this organism, Geobacillus LC300 was grown to high cell-densities in a fed-batch culture, where cells maintained a high xylose utilization rate under low dissolved oxygen concentrations. All of these characteristics make Geobacillus LC300 an attractive host for future metabolic engineering and biotechnology applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium

Cordova

Long

Venkataramanan

et al. 2015

Metabolic Engineering

View full text Add to dashboard Cite

show abstract

“…Although there is no evidence for a mutual relationship between TNO-09.020 and TNO-09.006, our observation may bear some resemblance to that of the yogurt consortium, where the proteolytic activity of L. bulgaricus results in the supply of amino acids for Streptococcus thermophilus (24). The ecology and interrelationship between the selected isolates will be elucidated using gene trait-matching approaches based on wholegenome sequence information (20,21). Such information would be relevant because our results suggest that the presence of proteolytic microorganisms in the dairy concentrate production line may contribute to the diversity and spore load of specific thermophiles in end products.…”

Section: Discussionmentioning

confidence: 99%

“…The percentage of relatedness to the type strain matched the Ͼ70% criterion for the assignment of all three bacterial species ( Table 1). The sequences of the full genomes of the three strains were determined (20,21), and the strains were characterized regarding their temperature growth range and optimum, sporulation efficiency, and spore heat resistance (Tables 1 and 2). In addition, their ability to sporulate was confirmed by microscopic examination, showing the formation of bright-phase endospores at the poles (see Fig.…”

Section: Enrichment Ofmentioning

confidence: 99%

Abiotic and Microbiotic Factors Controlling Biofilm Formation by Thermophilic Sporeformers

Zhao

Caspers

Metselaar

et al. 2013

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

e One of the major concerns in the production of dairy concentrates is the risk of contamination by heat-resistant spores from thermophilic bacteria. In order to acquire more insight in the composition of microbial communities occurring in the dairy concentrate industry, a bar-coded 16S amplicon sequencing analysis was carried out on milk, final products, and fouling samples taken from dairy concentrate production lines. The analysis of these samples revealed the presence of DNA from a broad range of bacterial taxa, including a majority of mesophiles and a minority of (thermophilic) sporeforming bacteria. Enrichments of fouling samples at 55°C showed the accumulation of predominantly Brevibacillus and Bacillus, whereas enrichments at 65°C led to the accumulation of Anoxybacillus and Geobacillus species. Bacterial population analysis of biofilms grown using fouling samples as an inoculum indicated that both Anoxybacillus and Geobacillus preferentially form biofilms on surfaces at air-liquid interfaces rather than on submerged surfaces. Three of the most potent biofilm-forming strains isolated from the dairy factory industrial samples, including Geobacillus thermoglucosidans, Geobacillus stearothermophilus, and Anoxybacillus flavithermus, have been characterized in detail with respect to their growth conditions and spore resistance. Strikingly, Geobacillus thermoglucosidans, which forms the most thermostable spores of these three species, is not able to grow in dairy intermediates as a pure culture but appears to be dependent for growth on other spoilage organisms present, probably as a result of their proteolytic activity. These results underscore the importance of abiotic and microbiotic factors in niche colonization in dairy factories, where the presence of thermophilic sporeformers can affect the quality of end products.

show abstract

“…Therefore, G. thermoglucosidans is a good candidate for screening the glucan branching enzyme. Based on the complete genome sequence of G. thermoglucosidans [Zhao et al, 2012], a gene putatively encoding a novel branching enzyme was identified. This report describes the cloning of the G. thermoglucosidans geneencoding branching enzyme, as well as its expression in Escherichia coli .…”

Section: Introductionmentioning

confidence: 99%

“…The bacterium Geobacillus thermoglucosidans was recently isolated from the soil around a dairy production plant [Zhao et al, 2012]. It is classified as a thermophile because its optimal growth occurs at approximately 60 ° C. This organism can also live in a wide variety of environments [Suzuki et al, 1983].…”

Section: Introductionmentioning

confidence: 99%

Expression and Biochemical Characterization of a Thermostable Branching Enzyme from <b><i>Geobacillus thermoglucosidans</i></b>

Ban

Li²,

Gu³

et al. 2016

Microb Physiol

View full text Add to dashboard Cite

The branching enzyme (EC 2.4.1.18) catalyzes the formation of α-1,6 branch points in starch. In this study, the Geobacillus thermoglucosidans gene-encoding branching enzyme was expressed in Escherichia coli BL21 (DE3) and the protein was isolated and characterized. G. thermoglucosidans branching enzyme is a thermostable enzyme with an optimal reaction temperature of nearly 60°C and a half-life at 65°C of approximately 1.1 h. The activity of the recombinant enzyme is optimal at pH 7.5, with broad stability between pH 5.5 and 9.0. Its thermostability, relatively broad pH stability and optimal temperature near the temperature at which starch begins to gelatinize may make it easy to use in industrial production. Furthermore, the enzyme is activated by Mg²⁺, Ba²⁺, K⁺ and Na⁺ in a concentration-dependent manner and dramatically inhibited by Ni²⁺ and Co²⁺. Its substrate dependence, using amylopectin as the substrate, could be adequately fitted using the Michaelis-Menten equation, yielding a K_m of 0.99 mg/ml. High-performance anion exchange chromatography results showed that the chain length distribution of branching enzyme-treated waxy corn starch is indistinguishable from that of the branching enzyme-treated common corn starch. This enzyme may therefore be a promising tool for the enzymatic modification of starch.

show abstract

Complete Genome Sequence of Geobacillus thermoglucosidans TNO-09.020, a Thermophilic Sporeformer Associated with a Dairy-Processing Environment

Cited by 35 publications

References 6 publications

Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium

Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium

Abiotic and Microbiotic Factors Controlling Biofilm Formation by Thermophilic Sporeformers

Expression and Biochemical Characterization of a Thermostable Branching Enzyme from <b><i>Geobacillus thermoglucosidans</i></b>

Contact Info

Product

Resources

About