Complete genome sequence of Bacillus cereus FORC_005, a food-borne pathogen from the soy sauce braised fish-cake with quail-egg

Abstract

Due to abundant contamination in various foods, the pathogenesis of Bacillus cereus has been widely studied in physiological and molecular level. B. cereus FORC_005 was isolated from a Korean side dish, soy sauce braised fish-cake with quail-egg in South Korea. While 21 complete genome sequences of B. cereus has been announced to date, this strain was completely sequenced, analyzed, and compared with other complete genome sequences of B. cereus to elucidate the distinct pathogenic features of a strain isolated in South Korea. The genomic DNA containing a circular chromosome consists of 5,349,617-bp with a GC content of 35.29 %. It was predicted to have 5170 open reading frames, 106 tRNA genes, and 42 rRNA genes. Among the predicted ORFs, 3892 ORFs were annotated to encode functional proteins (75.28 %) and 1278 ORFs were predicted to encode hypothetical proteins (748 conserved and 530 non-conserved hypothetical proteins). This genome information of B. cereus FORC_005 would extend our understanding of its pathogenesis in genomic level for efficient control of its contamination in foods and further food poisoning.

Genome Sequence of Bacillus cereus FORC_021, a Food-Borne Pathogen Isolated from a Knife at a Sashimi Restaurant

“…S3A) contained internalin-J (FORC21_3525), which can play an important role in host cell invasion (Fig. S3B) [20]. The fourth different genomic region in FORC_021 (ranging from FORC21_5078 to FORC21_5080; 5,036,952-5,042,545 bp) detected in a comparative genomic analysis between FORC_021 and ATCC 14579 T (Fig.…”

“…Sphingomyelinase, a virulence factor that interacts with non-hemolytic enterotoxin in insects and murine intestinal epithelial cells [8], was encoded by a single CDS (FORC21_0599). Four CDSs (FORC21_0480, FORC21_1291, FORC21_1550, and FORC21_3385) were annotated to the putative internalin, which plays an important role in host cell invasion [20]. A total of 89 CDSs in the contig were homologous to spore-forming proteins, which help survive against heat or acids.…”

“…Keywords: Bacillus cereus, food-borne pathogen, virulence factor, enterotoxin, Listeria pathogenicity island 1 (LIPI-1) S S comparison of entire genomes [12,17], and therefore, obtaining genome sequences of isolated strains is crucial to identify their characteristics. Several studies have analyzed the B. cereus genomes to understand its toxicity [3,16,20,24].…”

Species Dependence of SYTO 9 Staining of Bacteria

“…Bacterial nucleic acid content was considered with respect to observed differences in SYTO 9 fluorescence. The bacterial chromosomes of B. cereus, S. aureus, E. coli , and S. Typhimurium are approximately 5.3 Mbp ( Lee et al, 2015 ), 2.8 Mbp ( Baba et al, 2008 ; Makarova et al, 2017 ), 5.1 Mbp ( Minogue et al, 2014 ), and 4.8 Mbp ( Mcclelland et al, 2001 ), and the guanine-cytosine (G+C) content is 35, 32, 50, and 51%, respectively. The G+C content was examined in addition to cell number and nucleic acid content, to determine if base pair preferential binding would explain any observed differences between staining conditions.…”

Genome Structure ofBacillus cereustsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis

“…To generate an alignment map of the assembled tsu1 scaffolds (GenBank: KN321896-KN321931) using MUMmer (version 3.0) on the galaxy working station (biou.psc.edu/galaxy) [28], the genome sequence of B. cereus B4264 (NCBI GenBank: CP001176.1) with the highest identity similarity clustered by phylogenetic COG (PCOGR) was downloaded from NCBI database and used as the reference ( Figure S1 in Supplementary Material, available online at https://doi.org/10.1155/2017/6192924) [29]. Based on the alignment, 20 scaffolds of tsu1 were selected to construct a circular genomic map using DNAPlotter (version 10.2) [30,31]. Localizations of annotated genes on these scaffolds and on the reconstructed circular map were described in Supplementary Table S1.…”