Complete genome sequence of Achromobacter insolitus type strain LMG 6003T, a pathogen isolated from leg wound

Abstract

Achromobacter insolitus is a newly described, Gram-negative, small (1-2 mm long) and coccoid pathogen that has been isolated from leg wound. Due to its potential threat to human beings, the type strain of this species LMG 6003T was completely sequenced in this study. The genomic analysis revealed that the genome of LMG 6003T consists of one circular DNA chromosome of 6 492 697 bp with a GC content of 65.10%. The entire genome contains 6208 predicted coding genes, 61 tRNAs and 13 rRNA genes. Comparative genome analysis between LMG 6003T and another selected 10 sequenced Achromobacter revealed that 725 genes only exist in LMG 6003T. In particular, these genes include several important pathogenic-related genes such as Type IV prepilin peptidase (TadV/CpaA), Type VI secretion lipoprotein (VasD) and type 1 fimbriae major subunit (FimA). Genomic island (GI) analysis also suggests the existence of GIs in LMG 6003T. All these results strongly suggest the unique genomic features in LMG 6003T against other Achromobacter species. This report will provide us with an extended understanding of A. insolitus at the genomic level and would be helpful for understanding the evolution of the Achromobacter genus.

Comprehensive screening of genomic and metagenomic data reveals a large diversity of tetracycline resistance genes

“…Moreover, several of the predicted new efflux pump genes were located in opportunistic or pathogenic bacteria, e.g. Acinetobacter baumannii, Achromobacter insolitus, O. anthropi, Pandoraea pulmonicola and Burkholderia cenocepacia [66][67][68][69][70]. Among these, Acinetobacter baumannii is a notorious pathogen harbouring a wide range of mobile resistance genes against clinically relevant antibiotics, including tetracyclines [71].…”

Exploring the Bacteriome and Resistome of Humans and Food-Producing Animals in Brazil

“…In addition to Enterobacterales , we observed that environmental or commensal proteobacteria, such as C. kerstersii ( 32 , 33 ), A. insolitus ( 34 ), and B. hinzii ( 35 ), were abundant in both animals and humans and have been reported to be opportunistic agents colonizing humans. Microorganisms from distinct genera, such as P. stutzeri , Clostridium ventriculi , Faecalibacterium prausnitzii , and Bilophila wadsworthia , accounted for a proportion similar to those of some well-described antimicrobial-resistant species.…”

Effects of genetic and environmental variables on biofilm development dynamics in Achromobacter mucicolens

“…These various genes were investigated for their potential roles in biofilm development on other Achromobacter strains of A. insolitus and A. xylosoxidans (Table 1). These genes linked to biofilm development have been curated from Li et al, Nielsen SM et al (21)(22)(23).…”