Complete Genome Sequence of a Parabacteroides distasonis Strain (CavFT hAR46) Isolated from a Gut Wall-Cavitating Microlesion in a Patient with Severe Crohn’s Disease

Abstract

Crohn’s disease (CD) is a chronic inflammatory bowel disease (IBD) of the digestive tract in humans. There is evidence that Parabacteroides distasonis could contribute to IBD. Here, we present the complete genome sequence of a strain designated CavFT-hAR46, which was isolated from a gut intramural cavernous fistulous tract (CavFT) microlesion in a CD patient.

Parabacteroides distasonis: intriguing aerotolerant gut anaerobe with emerging antimicrobial resistance and pathogenic and probiotic roles in human health

“…For example, gelatin hydrolysis and collagenase tests may suggest a lack of proteolytic activity in P. distasonis, but, as previously mentioned, we have recovered this species from micro cavitating (fistulizing) lesions in the gut wall of Crohn's disease resected bowels, supporting the potential for proinflammatory activity in IBD. 97 Additional tests and growth assays will be performed to confirm this observation. As of January 12, 2021, there are 133 genomes for P. distasonis registered in the bioinformatics resource database PATRIC ver.…”

“…Nagayama et al 96 demonstrated that P. distasonis, along with nine other anaerobic bacteria cultured from the small intestinal mucosa of CD patients, enhanced T H 1 and T H 17 cell accumulation and intestinal inflammation. 96 Furthermore, we have recently identified P. distasonis in the deep-gut wall tissues from patients that underwent surgery for the removal of chronically inflamed bowel segments, specifically patients afflicted with CD, 97 supporting a probable pathologic role for this species in CD. This corroberates the observed enrichment of Bacteroidetes in the gut metagenome of the SAMP mouse line, which is naturally prone to CD-like ileitis.…”

“…We have identified that this strain exhibits an 80% match to other P. distasonis strains, including strains P. distasonis ATCC 8503 and 82G9, both isolated from the feces of patients. 97 Due to horizontal gene transfer of antimicrobial resistance genes, Bacteroides and Parabacteroides isolates from European and American patients now show tetracycline and erythromycin resistance, complicating genetic selection processes. Furthermore, while genetic manipulation via CRISPR-Cas9 can be done on Bacteroidales, offtarget mutations, inefficient transformation, and the need for strain-specific modifications prevent CRISPR-Cas9 from being an effective method for molecular-level genetic manipulation.…”

In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice

“…A particular strain (CavFT-hAR46) has been isolated from a gut intramural cavernous fistulous tract microlesion from the gut wall of a CD patient. The authors suggested it to be a potential pathogenic strain, however they did not demonstrate its capacity to promote intestinal inflammation [ 86 ]. Peptidoglycan recognition proteins ( Pglyrps ) have been shown to participate in maintaining intestinal microbiota and mice deficient in the encoding genes were more sensitive to colitis and presented an increased level of P. distasonis and Prevotella falsenii [ 59 ].…”

Dynamic Alterations of Gut Microbiota in Porcine Circovirus Type 3-Infected Piglets

“…Some reports suggest that Parabacteroides and Catenisphaera contribute to inflammatory diseases, such as inflammatory bowel disease (IBD) ( Kaakoush, 2015 ; Yang et al, 2019 ). In our study, Parabacteroides and Catenisphaera (members of the family Erysipelotrichaceae ) were markedly increased ( P < 0.01, P < 0.05, respectively) in PCV3-inoculated piglets at 14 dpi compared to sham-inoculated piglets ( Kanno et al, 2015 ).…”