COMPLEMENT-FIXING ANTIBRAIN ANTIBODIES IN MULTIPLE SCLEROSISA Preliminary Report

Abstract

Complement fixing antibrain antihodies were found in cerebrospinal fluid (CSF) from 6 of 12 patients with multiple sclerosis (MS), but not in any of 17 CSF specimens from patients with other neurological disorders when tested with a crude MS brain preparation as antigen. Complement fixing antihrain antibodies in lower titres were also found in 5 of the 6 positive MS CSF specimens when tested with a control brain preparation as antigen. The findings indicate the occurrence in MS of an immunization against brain components.

Antimyelin Antibodies in Neurologic Diseases

“…3. In selected experiments, the binding of specific immunoglobulin fractions of sera in undiluted and 1:2 dilutions was as¬ sayed, using subsequent incubation with -chain specific fluorescein-conjugated anti-IgG, anti-IgM, and anti-IgA.…”

Antimyelin Antibodies in Neurologic Diseases

“…3. In selected experiments, the binding of specific immunoglobulin fractions of sera in undiluted and 1:2 dilutions was as¬ sayed, using subsequent incubation with -chain specific fluorescein-conjugated anti-IgG, anti-IgM, and anti-IgA.…”

Effectors of Demyelination and Remyelination in the CNS: Implications for Multiple Sclerosis

“…There is also increasing evidence that complement is necessary for the effector arm of this immune response (62). Complement‐fixing antibodies have been demonstrated in MS (56). In addition, late components of the complement activation system are present in the cerebrospinal fluid (CSF) of MS patients (22).…”

Antimyelin Antibodies in Neurologic Diseases

“…3. In selected experiments, the binding of specific immunoglobulin fractions of sera in undiluted and 1:2 dilutions was as¬ sayed, using subsequent incubation with -chain specific fluorescein-conjugated anti-IgG, anti-IgM, and anti-IgA.…”

Effectors of Demyelination and Remyelination in the CNS: Implications for Multiple Sclerosis

“…There is also increasing evidence that complement is necessary for the effector arm of this immune response (62). Complement‐fixing antibodies have been demonstrated in MS (56). In addition, late components of the complement activation system are present in the cerebrospinal fluid (CSF) of MS patients (22).…”

Antimyelin Antibodies in Neurologic Diseases

“…3. In selected experiments, the binding of specific immunoglobulin fractions of sera in undiluted and 1:2 dilutions was as¬ sayed, using subsequent incubation with -chain specific fluorescein-conjugated anti-IgG, anti-IgM, and anti-IgA.…”

Effectors of Demyelination and Remyelination in the CNS: Implications for Multiple Sclerosis

“…There is also increasing evidence that complement is necessary for the effector arm of this immune response (62). Complement‐fixing antibodies have been demonstrated in MS (56). In addition, late components of the complement activation system are present in the cerebrospinal fluid (CSF) of MS patients (22).…”