Complement factor C5 in Atlantic salmon (Salmo salar): Characterization of cDNA, protein and glycosylation

Johansen, Wenche; Grove, Søren; Anonsen, Jan Haug; Moen, Anders; Agusti-Ridaura, Celia; Azar, Amir Sefidmouy; Strætkvern, Knut Olav

doi:10.1016/j.dci.2019.103424

Cited by 2 publications

(1 citation statement)

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“…Additionally, our proteomic results revealed differences between the diets, showing proteins detected exclusively in either FM or SBM group in DI and skin mucus. Among the proteins detected uniquely in DI mucus of fish fed SBM, we found complement C5, which is a crucial feature of the defensive complement in all vertebrates and has been recently characterized in Atlantic salmon (Johansen et al, 2019). Complement C5 is a key factor in a lytic cascade that can directly execute pathogens and plays an important part in host homeostasis and regulation of adaptive immunity and inflammation.…”

Section: Discussionmentioning

confidence: 69%

Immune and proteomic responses to the soybean meal diet in skin and intestine mucus of Atlantic salmon ( Salmo salar L.)

et al. 2021

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The main objective of this study was to increase the knowledge about the mucosal immunity of Salmo salar, using soybean meal‐induced enteritis as a model of inflammation. A control fish meal (FM) and a diet containing 20% soybean meal (SBM) were fed to salmon for seven weeks in seawater. There was no growth difference between groups. However, histology of distal intestine (DI) showed a mild inflammation in the fish fed SBM. Proteomic results revealed differences between the diets. Among the proteins detected uniquely in DI mucus of SBM group, complement C5, Galectin and Glutathione synthetase are involved in innate and adaptive immunity, inflammation, redox signalling and detoxification of xenobiotics in mammals, and similar roles are hypothesized in salmon. Adenylosuccinate synthetase and putative aminopeptidase were uniquely detected in the skin mucus of SBM group. Trypsin enzymatic activity was significantly decreased in the DI of SBM group. Significantly higher production of immunoglobulin M and Mucin‐like protein in DI mucus in SBM group was observed, while an increase in immunoglobulin D and lysozyme but decrease in chymotrypsin was detected in the skin mucus of the same group. We propose mucosal immunoglobulins as diagnostic biomarkers for assessment of novel feed ingredients and aquafeeds.

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Section: Discussionmentioning

confidence: 69%