Complement activation and protein adsorption by carbon nanotubes

Salvador-Morales, Carolina; Flahaut, Emmanuel; Sim, Edith; Sloan, Jeremy; Green, Malcolm L. H.; Sim, Robert B.

doi:10.1016/j.molimm.2005.02.006

Cited by 391 publications

(271 citation statements)

References 25 publications

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“…Single walled carbon nanotubes (SWCNT) interact strongly with a large range of molecular species from dyes to polysaccharides and bio-molecules (Bandyopadhyaya et al, 2002;Chambers et al, 2003;Casey et al, 2005;Dalton et al, 2000;Hedderman et al, 2004;Keogh et al, 2004;Moulton et al, 2005;Salvador-Morales et al, 2006). Spectroscopic analysis of a commercial cell culture medium, commonly used in toxicity studies, revealed significant interaction of the constituent components with SWCNT.…”

Section: Introductionmentioning

confidence: 99%

Single walled carbon nanotubes induce indirect cytotoxicity by medium depletion in A549 lung cells

Casey¹,

Herzog²,

Lyng³

et al. 2008

Toxicology Letters

162

113

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Single walled carbon nanotubes induce indirect cytotoxicity by medium depletion in A549 lung cells." Toxicology Letters, 179, 78-84 (2008) t r a c tThe ability of two types of single walled carbon nanotubes (SWCNT), namely Arc Discharge (AD) and HiPco ® single walled carbon nanotubes, to induce an indirect cytotoxicity in A549 lung cells by means of medium depletion was investigated. The nanotubes were dispersed in a commercial cell culture medium and subsequently removed by centrifugation and filtration. Spectroscopic analysis confirmed the removal of the nanotubes and showed differing degrees of alteration of the composition of the medium upon the removal of the nanotubes. The ability to induce an indirect cytotoxic effect by altering the medium was evaluated using two endpoints, namely the Alamar Blue (AB) and the Clonogenic assay. Exposure of the A549 cells to the depleted medium which had previously contained carbonaceous nanoparticles, revealed significant cytotoxicity for both endpoints employed. The results presented demonstrate that single walled carbon nanotubes can induce an indirect cytotoxicity by alteration of cell culture medium (in which they have previously been dispersed) which potentially results in a false positive toxic effect being observed in cytotoxicity studies.

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Section: Introductionmentioning

confidence: 99%

Single walled carbon nanotubes induce indirect cytotoxicity by medium depletion in A549 lung cells

Casey¹,

Herzog²,

Lyng³

et al. 2008

Toxicology Letters

162

113

View full text Add to dashboard Cite

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“…A central methodological problem is to separate free protein from protein bound to nanoparticles, ideally employing nonperturbing methods that do not disrupt the protein-particle complex or induce additional protein binding. The preferred method to-date has been centrifugation, identifying the major serum proteins albumin, IgG and fibrinogen as being associated with a wide range of particles of seemingly disparate molecular composition (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Due to its high abundance, albumin is almost always observed on particles and may be retrieved even if it has relatively low affinity.…”

mentioning

confidence: 99%

Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles

Cedervall¹,

Lynch²,

Lindman

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

2,755

2,561

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Due to their small size, nanoparticles have distinct properties compared with the bulk form of the same materials. These properties are rapidly revolutionizing many areas of medicine and technology. Despite the remarkable speed of development of nanoscience, relatively little is known about the interaction of nanoscale objects with living systems. In a biological fluid, proteins associate with nanoparticles, and the amount and presentation of the proteins on the surface of the particles leads to an in vivo response. Proteins compete for the nanoparticle ''surface,'' leading to a protein ''corona'' that largely defines the biological identity of the particle. Thus, knowledge of rates, affinities, and stoichiometries of protein association with, and dissociation from, nanoparticles is important for understanding the nature of the particle surface seen by the functional machinery of cells. Here we develop approaches to study these parameters and apply them to plasma and simple model systems, albumin and fibrinogen. A series of copolymer nanoparticles are used with variation of size and composition (hydrophobicity). We show that isothermal titration calorimetry is suitable for studying the affinity and stoichiometry of protein binding to nanoparticles. We determine the rates of protein association and dissociation using surface plasmon resonance technology with nanoparticles that are thiol-linked to gold, and through size exclusion chromatography of protein-nanoparticle mixtures. This method is less perturbing than centrifugation, and is developed into a systematic methodology to isolate nanoparticle-associated proteins. The kinetic and equilibrium binding properties depend on protein identity as well as particle surface characteristics and size.

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“…Carbon nanotubes (CNTs), including single-walled carbon nanotubes (SWCNTs) and MWCNTs, have attracted much interest since Iijima [1,2] first reported in 1991. Since that time CNTs have been studied in a range of diverse applications including biological, chemical and electrical [3][4][5][6][7][8] owing to their unique mechanical, electronic and thermal properties [9,10].…”

mentioning

confidence: 99%

Cytotoxicity and genotoxicity of multi-walled carbon nanotubes with human ocular cells

Lü

Zhang

et al. 2013

Chin. Sci. Bull.

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Cytotoxicity and genotoxicity of plasma-modified multi-walled carbon nanotubes (MWCNTs), including hydroxyl-MWCNTs (MWCNT-OH), carboxyl-MWCNTs (MWCNT-COOH) and pristine MWCNTs, with human ocular cells (e.g. retinal pigment epithelium (RPE) cells) have been studied in this work. The addition of MWCNT-based materials caused few change in cell morphology while the presence of MWCNTs was observed inside the cells using transmission electron microscopy (TEM), suggesting possibility of MWCNTs passing through the cell membranes without damaging cells. Cell viability measurements suggested that MWCNT-COOH exhibited better biocompatibility than other MWCNT materials studied in this work. Lactate Dehydrogenase (LDH) release level was found to be less than 30% with all types of MWCNT-based materials. Reactive Oxygen Species (ROS) generation was visible but not severe with addition of nanotubes. A smaller oxidative stress level was obtained from MWCNT-COOH. Cell apoptosis was found to be less than 1.5% with addition of MWCNT-based materials. Particularly MWCNTs were found to be swallowed by cells and released by cells after 72 h without damaging cells, which may be considered as a potential vector for ocular genetic diseases. Plasma modification of MWCNTs particularly with -COOH was found to be an efficient way to improve ocular biocompatibility of MWCNTs, suggesting a fast and useful way to modify MWCNTs for applications in areas such as biology and biomedicine. multi-walled carbon nanotubes, ocular biocompatibility, retinal pigment epithelium (RPE) cells, cytotoxicity, genotoxicity Citation:Yan L, Li G X, Zhang S, et al. Cytotoxicity and genotoxicity of multi-walled carbon nanotubes with human ocular cells.

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Complement activation and protein adsorption by carbon nanotubes

Cited by 391 publications

References 25 publications

Single walled carbon nanotubes induce indirect cytotoxicity by medium depletion in A549 lung cells

Single walled carbon nanotubes induce indirect cytotoxicity by medium depletion in A549 lung cells

Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles

Cytotoxicity and genotoxicity of multi-walled carbon nanotubes with human ocular cells

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