Competitive Promoter Occupancy by Two Yeast Paralogous Transcription Factors Controlling the Multidrug Resistance Phenomenon

Lucau-Danila, Anca; Delaveau, Thierry; Lelandais, Gaëlle; Devaux, Frédéric; Jacq, Claude

doi:10.1074/jbc.m309580200

Cited by 53 publications

(54 citation statements)

References 33 publications

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“…Parallel experiments were conducted in different genetic contexts. The wildtype strain was thus compared with strains in which the following genes coding for different transcription factors connected to the drug response were deleted: Yap1, Pdr1, Pdr3, Yrr1 (19), Pdr8 (15), and Yrm1 (20). Only the strains in which yap1 and pdr1 were deleted are considered in the rest of this work; deletions of the other genes did not lead to significant alterations of the genome-wide transcription response to benomyl under the conditions of this study.…”

Section: Resultsmentioning

confidence: 99%

Early Expression of Yeast Genes Affected by Chemical Stress

Lucau-Danila

Lelandais

Kozovská

et al. 2005

Molecular and Cellular Biology

108

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The variety of environmental stresses is probably the major challenge imposed on transcription activators and the transcriptional machinery. To precisely describe the very early genomic response developed by yeast to accommodate a chemical stress, we performed time course analyses of the modifications of the yeast gene expression program which immediately follows the addition of the antimitotic drug benomyl. Similar analyses were conducted with different isogenic yeast strains in which genes coding for relevant transcription factors were deleted and coupled with efficient bioinformatics tools. Yap1 and Pdr1, two well-known key mediators of stress tolerance, appeared to be responsible for the very rapid establishment of a transient transcriptional response encompassing 119 genes. Yap1, which plays a predominant role in this response, binds, in vivo, promoters of genes which are not automatically up-regulated. We proposed that Yap1 nuclear localization and DNA binding are necessary but not sufficient to elicit the specificity of the chemical stress response.Cellular organisms develop a myriad of strategies to maintain specific internal conditions constantly challenged by the varying drug environment. The complexity of the yeast cell system for detecting and responding to environmental variations is only beginning to come to light. It has been reported previously (13) that a large set of yeast genes (about 900) showed a similar drastic response to a large variety of environmental changes including temperature shocks, hydrogen peroxide, menadione, diamide, dithiothreitol, hyper-or hypoosmotic shock, amino acid starvation, nitrogen source depletion, and progression into stationary phase. Since these pioneering studies were reported, many observations of the global effects of a large variety of drugs on gene expression have been made. In most of these studies, a binary comparison (i.e., control versus stress-exposed cells) was carried out, whereas in some cases, time course experiments over rather long periods (several hours) were conducted. Although much valuable information has been collected in these studies, the heterogeneity in the protocols followed precludes a simple comparison between the different drug responses. In particular, it is extremely difficult to identify the different regulatory networks and to establish their chronological relationships. Time series experiments soon appeared and were much more informative than simple binary experiments. Such approaches were a particularly valuable source of information in the case of cell cycle analyses (24, 27); however, they were less suitable to describe the chronology of transcriptional events in the case of environ-

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Section: Resultsmentioning

confidence: 99%

Early Expression of Yeast Genes Affected by Chemical Stress

Lucau-Danila

Lelandais

Kozovská

et al. 2005

Molecular and Cellular Biology

108

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“…We reasoned that carotenoids, which are hydrophobic compounds (Gruszecki and Strzalka, 2005), cannot readily dissolve in the media used for the growth of yeast strains but are more likely to remain attached to cellular membranes. To determine whether carotenoids could be secreted from Orange02 cells during growth in batch cultures, 20% v/v sunflower oil, which is a proper solvent * * * 0 (DeRisi et al, 2000;Le Crom et al, 2002;Devaux et al, 2002;Hikkel et al, 2003;Tuttle et al, 2003;Lucau-Danila et al, 2003;Onda et al, 2004 Nawrocki et al, 2001;Hikkel et al, 2003;Tuttle et al, 2003;Onda et al, 2004) PDR15 2 (Wolfger et al, 1997;DeRisi et al, 2000;Hikkel et al, 2003;Tuttle et al, 2003) YLR346C 3 (DeRisi et al, 2000;do Valle Matta et al, 2001;Le Crom et al, 2002;Devaux et al, 2002;Hikkel et al, 2003;Tuttle et al, 2003;Onda et al, 2004) PDR5 6 (DeRisi et al, 2000Miura et al, 2001;Devaux et al, 2002;Teixeira and Sa-Correia, 2002;Tuttle et al, 2003;Lucau-Danila et al, 2003;Onda et al, 2004;Wehrschutz-Sigl et al, 2004 DeRisi et al, 2000;Le Crom et al, 2002;Devaux et al, 2002;Hikkel et al, 2003;Tuttle et al, 2003;Lucau-Danila et al, 2003;Onda et al, 2...…”

Section: Carotenoid Secretion From Carotenoid-producing S Cerevisiaementioning

confidence: 99%

Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drug resistance stress response

Verwaal

Jiang

Wang

et al. 2010

Yeast

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To obtain insight into the genome-wide transcriptional response of heterologous carotenoid production in Saccharomyces cerevisiae, the transcriptome of two different S. cerevisiae strains overexpressing carotenogenic genes from the yeast Xanthophyllomyces dendrorhous grown in carbon-limited chemostat cultures was analysed. The strains exhibited different absolute carotenoid levels as well as different intermediate profiles. These discrepancies were further sustained by the difference of the transcriptional response exhibited by the two strains. Transcriptome analysis of the strain producing high carotenoid levels resulted in specific induction of genes involved in pleiotropic drug resistance (PDR). These genes encode ABC-type and major facilitator transporters which are reported to be involved in secretion of toxic compounds out of cells. β-Carotene was found to be secreted when sunflower oil was added to the medium of S. cerevisiae cells producing high levels of carotenoids, which was not observed when added to X. dendrorhous cells. Deletion of pdr10, one of the induced ABC transporters, decreased the transformation efficiency of a plasmid containing carotenogenic genes. The few transformants that were obtained had decreased growth rates and lower carotenoid production levels compared to a pdr5 deletion and a reference strain transformed with the same genes. Our results suggest that production of high amounts of carotenoids in S. cerevisiae leads to membrane stress, in which Pdr10 might play an important role, and a cellular response to secrete carotenoids out of the cell.

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“…YRM1 (yeast reveromycin resistance modulator) encodes another Zn(II) 2 Cys 6 regulator acting as a specific inhibitor of Yrr1p. In the absence of Yrr1p, Yrm1p activates the transcription of most genes regulated by Yrr1p, reflecting the high level of complexity of the regulatory processes controlling drug resistance phenotypes in yeast [71].…”

Section: Regulation Of Abc Gene Expression In S Cerevisiaementioning

confidence: 99%

Yeast ABC transporters – A tale of sex, stress, drugs and aging

2005

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Yeast ATP-binding cassette (ABC) proteins are implicated in many biological phenomena, often acting at crossroads of vital cellular processes. Their functions encompass peptide pheromone secretion, regulation of mitochondrial function, vacuolar detoxification, as well as pleiotropic drug resistance and stress adaptation. Because yeast harbors several homologues of mammalian ABC proteins with medical importance, understanding their molecular mechanisms, substrate interaction and three-dimensional structure of yeast ABC proteins might help identifying new approaches aimed at combating drug resistance or other ABC-mediated diseases. This review provides a comprehensive discussion on the functions of the ABC protein family in the yeast Saccharomyces cerevisiae.

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Competitive Promoter Occupancy by Two Yeast Paralogous Transcription Factors Controlling the Multidrug Resistance Phenomenon

Cited by 53 publications

References 33 publications

Early Expression of Yeast Genes Affected by Chemical Stress

Early Expression of Yeast Genes Affected by Chemical Stress

Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drug resistance stress response

Yeast ABC transporters – A tale of sex, stress, drugs and aging

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