Competitive Chemiluminescent Immunoassay for Estradiol Using anN-Functionalized Acridinium Ester

Abstract

We have compared three competitive chemiluminescent immunoassays (CLIA) for estradiol (E2) using an N-functionalized acridinium ester (AE). The assays were a standard competitive assay using immobilized antibody and directly labeled antigen (type A), an immobilized antibody and indirectly labeled antigen (type B), and an immobilized antigen and labeled antibody (type C). In an antibody-immobilized system, the assay using both AE- and E2-labeled thyroglobulin as a tracer (type B) was more sensitive than that using AE directly coupled with E2 (type A). Subsequently, a comparison of the antibody-immobilized system (type B) and an antigen-immobilized system (type C) showed that the latter was slightly more sensitive than the former. The sensitivity of the CLIA (type C) was similar or superior to commercially available CLIA or radioimmunoassays for E2. Thus, the N-functionalized AE proved to be a useful labeling reagent for a competitive CLIA with high sensitivity.

Synthesis, structure elucidation, and chemiluminescent activity of new 9‐substituted 10‐(ω‐(succinimidyloxycarbonyl)alkyl)acridinium esters

“…1H), 7.03 (d, 8 Hz, 1H), 2.39 (s, 3H), 2.29 (s, 3H). 13 [26][27][28][29] To compound 2b (140 mg, 0.43 mmol), in dry DCM (2 ml), under…”

Production and Characterization of Monoclonal Antibodies against Acridine Ester

“…Acridine ester is a high effective label in chemiluminescence immunoassay (CLIA) [4], and is conjugated with amino, carboxyl or phosphate groups which are from biomacromolecule such as antigen, antibody and DNA for development of CLIA [5][6][7][8]. In 1983, Ian Weeks et al [9] improved CLIA using acridine ester as a label which has less background and better sensitivity [10][11][12][13]. The sensitivity, repeatability and accuracy of CLIA are preferable to ELISA [14].…”

Using acridinium ester as the sonochemiluminescent probe for labeling of protein

“…9,10 Acridinium ester has been usually used as the CL label in immunoassays due to its extremely high sensitivity and selectivity. [11][12][13][14][15] Since CL was firstly used in immunoassays, 12 a variety of configurations have been introduced in assays of clinical importance. Flow injection analysis (FIA) used in association with CL immunoassays has proved to offer advantages of faster speed and better reproducibility.…”