Competition between enzymatic and non-enzymatic electrochemical determination of cholesterol

Hefnawy, Mahmoud A.; Fadlallah, Sahar A.; El‐Sherif, Rabab M.; Medany, Shymaa S.

doi:10.1016/j.jelechem.2023.117169

Cited by 26 publications

(21 citation statements)

References 184 publications

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“…The electrochemical methods are reported to be highly sensitive and selective techniques for nitrite detection in contaminated water. Thus, there are many advantages, such as low cost, fast response, simplicity, and reproducibility [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Polyaniline-Supported Nickel Oxide Flower for Efficient Nitrite Electrochemical Detection in Water

et al. 2023

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A modified electrode with conducting polymer (Polyaniline) and NiO nanoflowers was prepared to detect nitrite ions in drinking water. A simple method was used to prepare the NiO nanoflower (NiOnF). Several techniques characterized the as-prepared NiOnF to determine the chemical structure and surface morphology of the NiO, such as XRD, XPS, FT-IR, and TGA. The activity of the electrode toward nitrite sensing was investigated over a wide range of pH (i.e., 2 to 10). The amperometry method was used to determine the linear detection range and limit. Accordingly, the modified electrode GC/PANI/NiOnf showed a linear range of detection at 0.1–1 µM and 1–500 µM. At the same time, the limit of detection (LOD) was 9.7 and 64 nM for low and high concentrations, respectively. Furthermore, the kinetic characteristics of nitrite, such as diffusion and transport coefficients, were investigated in various media. Moreover, the charge transfer resistance was utilized for nitrite electrooxidation in different pH values by the electrochemical impedance technique (EIS). The anti-interfering criteria of the modified surfaces were utilized in the existence of many interfering cations in water (e.g., K+, Na+, Cu2+, Zn2+, Ba2+, Ca2+, Cr2+, Cd2+, Pd2+). A real sample of the Nile River was spiked with nitrite to study the activity of the electrode in a real case sample (response time ~4 s). The interaction between nitrite ions and NiO{100} surface was studied using DFT calculations as a function of adsorption energy.

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Section: Introductionmentioning

confidence: 99%

Polyaniline-Supported Nickel Oxide Flower for Efficient Nitrite Electrochemical Detection in Water

et al. 2023

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“…6 In this context, due to the low cost of both materials sources and production, as well as versatile and easily adjustable design, non-enzymatic electrochemical sensors are a relevant option for the analysis of cholesterol and other biomolecules. 7,8 To enhance the electrochemical response of non-enzymatic sensors, electrodes can be modified with metallic species to achieve high sensitivity, 9 for example, silver, 10 gold 11 and nickel, 12 and be explored for the determination of cholesterol. Nickel has been widely used in non-enzymatic electrochemical sensors for cholesterol determination due to its low cost compared to noble metals, high stability in alkaline solutions, and reproducibility.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical microfluidic device for non-enzymatic cholesterol determination using a lab-made disposable electrode

et al. 2023

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“…Similarly, gas chromatography-mass spectrometry [6], and HPLC [7] involve high instrument costs, skilled operators, chemical solvents, difficult sample preparation, and extended analysis times [8]. Electrochemical sensors [9], on the other hand, are becoming more relevant as bioanalytical sensing instruments for identifying CH and offer a range of benefits, including low cost, rapid testing times, portability, miniaturization, and high sensitivity [10]. There are two types of electrochemical methods for detecting CH: enzymatic and nonenzymatic [11].…”

Section: Introductionmentioning

confidence: 99%

Tin oxide-polyaniline nanocomposite modified nickel foam for highly selective and sensitive detection of cholesterol in simulated blood serum samples

Singh¹,

Sreekumar²,

Badhulika³

2023

Nanotechnology

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Cholesterol (CH) is a vital diagnostic marker for a variety of diseases, making its detection crucial in biological applications including clinical practice. In this work, we report the synthesis of tin oxide-polyaniline nanocomposite-modified nickel foam (SnO2-PANI/NF) for non-enzymatic detection of CH in simulated human blood serum. SnO2 was synthesized via the hydrothermal method, followed by the synthesis of SnO2-PANI nanocomposite through in situ chemical polymerization of aniline using ammonium persulfate as the oxidizing agent. Morphological studies display agglomerated SnO2-PANI, which possess diameters ranging from an average particle size of ∼50 to ∼500 nm, and the XRD analysis revealed the tetragonal structure of the SnO2-PANI nanocomposite. Optimization studies demonstrating the effect of pH and weight percentage are performed to improve the electrocatalytic performance of the sensor. The non-enzymatic SnO2-PANI/NF sensor exhibits a linear range of 1–100 μM with a sensitivity of 300 μA μM−1/cm−2 towards CH sensing and a low limit of detection of 0.25 μM (=3 S m−1). SnO2-PANI/NF facilitates the electrooxidation of CH to form cholestenone by accepting electrons generated during the reaction and transferring them to the nickel foam electrode via Fe (III)/Fe (IV) conversion, resulting in an increased electrochemical current response. The SnO2-PANI/NF sensor demonstrated excellent selectivity against interfering species such as Na+, Cl−, K+, glucose, ascorbic acid, and SO4 2−. The sensor successfully determined the concentration of CH in simulated blood serum samples, demonstrating SnO2-PANI as a potential platform for a variety of electrochemical-based bioanalytical applications.

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Competition between enzymatic and non-enzymatic electrochemical determination of cholesterol

Cited by 26 publications

References 184 publications

Polyaniline-Supported Nickel Oxide Flower for Efficient Nitrite Electrochemical Detection in Water

Polyaniline-Supported Nickel Oxide Flower for Efficient Nitrite Electrochemical Detection in Water

An electrochemical microfluidic device for non-enzymatic cholesterol determination using a lab-made disposable electrode

Tin oxide-polyaniline nanocomposite modified nickel foam for highly selective and sensitive detection of cholesterol in simulated blood serum samples

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