Compartmentalized reactions as a case of soft-matter biotechnology: synthesis of proteins and nucleic acids inside lipid vesicles

Stano, Pasquale; Carrara, Paolo; Kuruma, Yutetsu; Souza, Tereza Pereira de; Luisi, Pier Luigi

doi:10.1039/c1jm12298c

Cited by 132 publications

(107 citation statements)

References 151 publications

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“…Liposomes were also purified using an anionic exchange column to remove unencapsulated T7RNAP and DNA (Supporting Information, Figure S3). This quick, single-step method for purification is an alternative to published methods of adding ethylenediaminetetraacetic acid (EDTA) or nucleases to inhibit transcription outside of liposomes, [5,15] which are likely unsuitable for subsequent cellular delivery.…”

Section: Resultsmentioning

confidence: 99%

“…These systems have been encapsulated within lipid bilayers to form "protocells", liposomes capable of protein expression. [3] Protocells have been used to model early cellular life, [4,5] and significant advances have been made in studying and maximizing protein expression within nano- [6] and microsized liposomes. [7,8] Several applications for protocells in synthetic biology and drug delivery have been explored.…”

Section: Introductionmentioning

confidence: 99%

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Controlled Transcription of Exogenous mRNA in Platelets Using Protocells

et al. 2015

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Transcribing exogenous RNA in eukaryotic cells requires delivering DNA to their nuclei and changing their genome. Nuclear delivery is often inefficient, limiting the potential scope of gene therapy and synthetic biology. These challenges may be overcome by techniques that allow for extranucleate transcription within eukaryotic cells. Protocells have been developed that enable transcription inside of liposomes; however, it has not yet been demonstrated whether this technology can be extended for use within eukaryotic cells. Here we show RNA-synthesizing nanoliposomes allow transcription of exogenous RNA inside anucleate cells. To accomplish this, components of transcription were encapsulated into liposomes and delivered to platelets. These liposomes were capable of light-induced transcription in platelets, providing proof-of-concept that protocell technology can be adapted for use within mammalian cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Controlled Transcription of Exogenous mRNA in Platelets Using Protocells

et al. 2015

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“…Oparin never solved the first problem; he succeeded in preparing coacervate droplets with enzymes (which, due to accumulation of products, even divided themselves), but never with more than one enzyme. 26 Lately, much more elaborate enzymatic systems have been devised; those based on phospholipid vesicles were even able to synthesize proteins and nucleic acids, 27,28 but the problem of regulation persisted. The second problem, the evolution of catalysis inside the droplets, has two aspects: (1) catalysis of protein synthesis, and (2) abiotic generation of catalytic peptides.…”

Section: Oparin's Solution: Homogeneous Catalysis In Coacervate Dropletsmentioning

confidence: 99%

Catalysis in the Primordial World

Raos

Bermanec²

2017

Kem. Ind.

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Catalysis provides orderly prebiotic synthesis and eventually its evolution into autocatalytic (self-reproduction) systems. Research on homogeneous catalysis is concerned mostly with random peptide synthesis and the chances to produce catalytic peptide oligomers. Synthesis of ribose via formose reaction was found to be catalysed by B(OH) 4 − , presumably released by weathering of borate minerals. Oxide and clay mineral surfaces provide catalytic sites for the synthesis of oligopeptides and oligonucleotides. Chemoautotrophic or iron-sulphur-world theory assumes that the first (pioneer) organisms developed by catalytic processes on (Fe/Ni)S particles formed near/close hydrothermal vents. The review provides an overlay of possible catalytic reactions in prebiotic environment, discussing their selectivity (regioselectivity, stereoselectivity) as well as geological availability of catalytic minerals and geochemical conditions enabling catalytic reactions on early Earth.

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“…SSMCs can currently produce simple water-soluble functional proteins and some membrane proteins, which are often required for sensoring purposes, e.g. detecting molecules outside cells and trigger internal responses (see [54,29,8] and references therein).…”

Section: Semi-synthetic Minimal Cells (Ssmcs)mentioning

confidence: 99%

From Cells as Computation to Cells as Apps

Bracciali

Cataldo

Damiano

et al. 2016

IFIP Advances in Information and Communication Technology

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Abstract. We reflect on the computational aspects that are embedded in life at the molecular and cellular level, where life machinery can be understood as a massively distributed system whose macroscopic behaviour is an emerging property of the interaction of its components. Such a relatively new perspective, clearly pursued by systems biology, is contributing to the view that biology is, in several respects, a quantitative science. The recent developments in biotechnology and synthetic biology, noticeably, are pushing the computational interpretation of biology even further, envisaging the possibility of a programmable biology. Several in-silico, in-vitro and in-vivo results make such a possibility a very concrete one. The long-term implications of such an "extended" idea of programmable living hardware, as well as the applications that we intend to develop on those "computers", pose fundamental questions.

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Compartmentalized reactions as a case of soft-matter biotechnology: synthesis of proteins and nucleic acids inside lipid vesicles

Cited by 132 publications

References 151 publications

Controlled Transcription of Exogenous mRNA in Platelets Using Protocells

Controlled Transcription of Exogenous mRNA in Platelets Using Protocells

Catalysis in the Primordial World

From Cells as Computation to Cells as Apps

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