Compartmental organization of Purkinje cells in the mature and developing mouse cerebellum as revealed by an olfactory marker protein-lacZ transgene

Nunzi, Maria Grazia; Grillo, Mary; Margolis, Frank L.; Mugnaini, Enrico

doi:10.1002/(sici)1096-9861(19990201)404:1<97::aid-cne8>3.0.co;2-1

Cited by 25 publications

(1 citation statement)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we still do not fully understand how developmental clusters transform into mature zones with well-defined boundaries. From approximately embryonic day (E) 14, Purkinje cell clusters begin to express a variety of molecular markers that compartmentalize the developing cerebellum into an array of nascent sagittal zones (Wassef and Sotelo, 1984; Wassef et al, 1985; Oberdick et al, 1993; Millen et al, 1995; Nunzi et al, 1999; Ozol et al, 1999; Armstrong et al, 2001; Larouche et al, 2006; Furutama et al, 2010; Redies et al, 2011). Although we now know that the adult pattern reflects a complicated correlate of the embryonic cluster map, and apparently is derived from approximately 50 clusters (Fujita et al, 2012), it is still not clear how the early postnatal plan transforms into the mature zonal map.…”

Section: Introductionmentioning

confidence: 99%

Postnatal development of cerebellar zones revealed by neurofilament heavy chain protein expression

White¹,

Sillitoe²

2013

Front. Neuroanat.

View full text Add to dashboard Cite

The cerebellum is organized into parasagittal zones that control sensory-motor behavior. Although the architecture of adult zones is well understood, very little is known about how zones emerge during development. Understanding the process of zone formation is an essential step toward unraveling how circuits are constructed to support specific behaviors. Therefore, we focused this study on postnatal development to determine the spatial and temporal changes that establish zonal patterns during circuit formation. We used a combination of wholemount and tissue section immunohistochemistry in mice to show that the cytoskeletal protein neurofilament heavy chain (NFH) is a robust marker for postnatal cerebellar zonal patterning. The patterned expression of NFH is initiated shortly after birth, and compared to the domains of several known zonal markers such as zebrin II, HSP25, neurogranin, and phospholipase Cβ4 (PLCβ4), NFH does not exhibit transient expression patterns that are typically remodeled between stages, and the adult zones do not emerge after a period of uniform expression in all lobules. Instead, we found that throughout postnatal development NFH gradually reveals distinct zones in each cerebellar lobule. The boundaries of individual NFH zones sharpen over time, as zones are refined during the second and third weeks after birth. Double labeling with neurogranin and PLCβ4 further revealed that although the postnatal expression of NFH is spatially and temporally unique, its pattern of zones respects a fundamental and well-known molecular topography in the cerebellum. The dynamics of NFH expression support the hypothesis that adult circuits are derived from an embryonic map that is refined into zones during the first 3-weeks of life.

show abstract

Section: Introductionmentioning

confidence: 99%

Postnatal development of cerebellar zones revealed by neurofilament heavy chain protein expression

White¹,

Sillitoe²

2013

Front. Neuroanat.

View full text Add to dashboard Cite

show abstract

Transverse zones in the vermis of the mouse cerebellum

et al. 1999

View full text Add to dashboard Cite

The mouse cerebellar cortex is subdivided by an elaborate array of parasagittal and transverse boundaries. The relationship between these two orthogonal patterns of compartmentation is understood poorly. We have combined the use of adult and perinatal molecular markers of compartmentation—zebrin II, calbindin, and an L7/pcp‐2‐lacZ transgene—to resolve some of these issues. Our results indicate that the adult cerebellar vermis is divided along the rostrocaudal axis by three transverse boundaries: through the rostral face of lobule VI, in the caudal half of lobule VII, and across the posterolateral fissure between lobules IX and X. These three boundaries subdivide the vermis into four transverse zones: the anterior zone (lobules I–V), the central zone (lobules VI–VII), the posterior zone (lobules VIII–IX), and the nodular zone (lobule X). The same zones and boundaries also can be identified in the newborn cerebellum. The parasagittal organization is different in each zone: a unique combination of Purkinje cell phenotypes is found in each transverse zone both in the neonate and the adult, and different zones have distinct developmental time tables. Furthermore, the parasagittal bands of Purkinje cells revealed in the adult cerebellar cortex by using antizebrin II immunocytochemistry are discontinuous across the transverse boundaries. These data suggest that the transverse zones of the vermis form first during development and that parasagittal compartmentation develops independently in each transverse zone. J. Comp. Neurol. 412:95–111, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

Constitutive expression of the 25-kDa heat shock protein Hsp25 reveals novel parasagittal bands of Purkinje cells in the adult mouse cerebellar cortex

et al. 2000

View full text Add to dashboard Cite

Compartmental organization of Purkinje cells in the mature and developing mouse cerebellum as revealed by an olfactory marker protein-lacZ transgene

Cited by 25 publications

References 86 publications

Postnatal development of cerebellar zones revealed by neurofilament heavy chain protein expression

Postnatal development of cerebellar zones revealed by neurofilament heavy chain protein expression

Transverse zones in the vermis of the mouse cerebellum

Constitutive expression of the 25-kDa heat shock protein Hsp25 reveals novel parasagittal bands of Purkinje cells in the adult mouse cerebellar cortex

Contact Info

Product

Resources

About