Comparison of verdeluz orange G and modified Gallego stains

Abstract

Tumors of the oral cavity include combinations of hard and soft tissues that may be difficult to identify using routine hematoxylin and eosin (H & E) staining. Although combination stains can demonstrate hard and soft tissues, trichrome stains, such as VanGieson and Masson, cannot differentiate dental hard tissues, such as dentin, cementum and osteoid. Modified Gallegos (MGS) and verdeluz orange G-acid fuchsin (VOF) stains can differentiate components of teeth. We used 10 tissue sections of decalcified bone and 10 pathologic tissue sections that contained different calcified tissues including peripheral ossifying fibroma, odontoma, central ossifying fibroma and cemento-ossifying fibroma. Sections were stained with H & E, VOF or MGS. H and E stained both hard tissues pink. VOF stained bone purple-red, cementum red and collagen blue. MGS stained bone green-blue, cementum red and collagen blue. VOF staining intensity and differentiation was better than MGS staining. VOF staining demonstrated hard tissue components distinctly and exhibited good contrast with the surrounding connective tissue. VOF also is a simple, single step, rapid staining procedure.

Natural stain (Kumkum) formulated by the extract of Curcuma aromatica and slaked lime in histostaining of oral tissues

“…The authors confirmed that VOF showed better staining intensity for hard tissues and exhibited good contrast with surrounding connective tissue. [ 15 ] In the present study a natural compound, Kumkum was found to differentiate immature from mature bone. Structures such as canaliculi of osteocytes, resting and reversal lines of bone had a better contrast with increased staining intensity.…”

Modified Gallego’s Stain as Differential Stain for The Assessment of Dentin Morphology

“…When stained with Modified Gallego's stain, these components were stained with different colours as follows (Sandhya et al, 2014). It stains dentin in dark blue color as shown in figure 4, the dentinal tubules were observed as dark blue striations as shown in figure 4 while the coronal dentin is stained in light blue color as shown in figure 5 and interglobular dentin looks violet blue after staining with modified Gallego's stain as shown in figure 6 (Kunche et al, 2017). This staining procedure is technique sensitive and the intensity of the colour in the stained sections decreases as the time duration increases and becomes difficult to differentiate between the dentin at different parts (Comparison of Verdeluz Orange G and Modified Gallego Stains, 2017).…”