Comparison of ultrastructure, tight junction-related protein expression and barrier function of human corneal epithelial cells cultivated on amniotic membrane with and without air-lifting

Ban, Yuriko; Cooper, Leanne J.; Fullwood, Nigel J.; Nakamura, Takahiro; Tsuzuki, Masato; Koizumi, Noriko; Dota, Atsuyoshi; Mochida, Chikako; Kinoshita, Shigeru

doi:10.1016/s0014-4835(03)00033-2

Cited by 98 publications

(58 citation statements)

References 27 publications

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“…21,23 Air-lifting was also shown to promote epithelial stratification and tight junction formation. 39 Ang et al demonstrated by using cultivated conjunctival epithelium that air-lifting caused reduced proliferating potential and it may be disadvantageous for long-term transplantation outcome. 40 Although longevity of the differentiated epithelial cell sheet can be a theoretical concern in long-term postoperative outcome, 17 the result of the current study indicated that the clinical outcomes were more feasible in eyes that had Method 2.…”

Section: Discussionmentioning

confidence: 99%

Factors Influencing Outcomes in Cultivated Limbal Epithelial Transplantation for Chronic Cicatricial Ocular Surface Disorders

Shimazaki

Higa

Morito

et al. 2007

American Journal of Ophthalmology

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Cultivated limbal epithelial transplantation (CLET) is a recently developedsurgical method to reconstruct the ocular surface in eyes with limbal dysfunction. The concept of the CLET is to produce a limbal epithelial sheet containing progenitor cells of the corneal epithelium, and then to transplant to the ocular surface following excision of cicatricial tissues. The method was first reported by Pellegrini et al in 1997, followed by several other investigators including our group. [1][2][3][4][5][6][7][8][9][10][11][12] In our previous report in 2002, the short-term outcomes of CLET were comparable with conventional limbal epithelial transplantation. 7 We found that the method did not produce excellent outcomes in patients with severe cicatricial ocular surface disorders such as Stevens-Johnson syndrome (SJS) and chemical/thermal burns of the cornea. 7 This may be due to the fact that many of the patients had risk factors for poor epithelialization such as decreased tear production, lid abnormalities, persistent inflammation. [13][14][15] It is not clear whether the relatively poor outcomes of our previous report can be improved by the modification of the epithelial sheet preparation. There are a variety of factors that may influence the quality of the epithelial sheet, which include; source of cells, preparation of the cells, types of substrates used, cultivation medium, the use of feeder cells, and culture conditions. Our group has made some modification of the cultivation/preparation methods including the use of feeder cells, cell suspension technique and air-lifting of the cell sheet.In order to investigate whether the modification resulted in improvement in surgical outcomes, we retrospectively studied the mid-term outcomes of CLET. The mid-term outcomes of CLET for chronic cicatricial ocular surface disorders were compared with the different cultivation methods. Also, other factors were analyzed to see if they influenced the results. METHODS PatientsTwenty nine eyes of 27 patients that had CLET between June 1999 and November 2003 were retrospectively analyzed in this study. Patients consisted of 16 males and 11 females, with a mean age of 50.2 + 20.7 years (15-82 years). All eyes had total limbal dysfunction. Conjunctivalization was confirmed preoperatively by impression cytology in 10 of 27 eyes. Mean corrected visual acuity was counting fingers, and two-third of the eyes had less than 20/2000. Schirmer`s test was performed in 21 eyes and decreased tear secretion (Schirmer`s value < 5 mm) was noted in 12 eyes. As for preoperative complications, glaucoma, macular degeneration, and lid deformity were noted in 4, 1, and 3 eyes, respectively.

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Section: Discussionmentioning

confidence: 99%

Factors Influencing Outcomes in Cultivated Limbal Epithelial Transplantation for Chronic Cicatricial Ocular Surface Disorders

Shimazaki

Higa

Morito

et al. 2007

American Journal of Ophthalmology

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“…These tight-junctions form an epithelial junctional complex with the adherens junctions and desmosomes to avoid the entry of substances within the eye (Ban et al, 2003). Tight junctions are multifunctional and dynamic protein complexes that connect adjacent cells by sealing the intercellular space between cells preventing the paracellular movement of solutes, ions and water.…”

Section: Effect On Epithelial Permeabilitymentioning

confidence: 99%

“…Tight junctions are multifunctional and dynamic protein complexes that connect adjacent cells by sealing the intercellular space between cells preventing the paracellular movement of solutes, ions and water. The main proteins forming a tight junction in the cornea are occludin (Langbein et al, 2002), claudins (Ban et al, 2003) and zonula occludens-1 (ZO-1) (Ban et al, 2003;Sosnova-Netukova et al, 2007;Yoshida et al, 2009). It was possible to measure the effect of Ap 4 A on tight junction proteins by western blot and the three mentioned proteins, claudin, occludin-7 and ZO-1, showed a clear reduction in its expression 2 h after the dinucleotide application, the fall of expression being 59.3% for ZO-1, 57.9% for occludin-7 and 58.8% for claudin (Loma et al, 2015b).…”

Section: Effect On Epithelial Permeabilitymentioning

confidence: 99%

The role of dinucleoside polyphosphates on the ocular surface and other eye structures

Carracedo

Crooke

Guzmán-Aránguez

et al. 2016

Progress in Retinal and Eye Research

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a b s t r a c tDinucleoside polyphosphates comprises a group of dinucleotides formed by two nucleosides linked by a variable number of phosphates, abbreviated NpnN (where n represents the number of phosphates). These compounds are naturally occurring substances present in tears, aqueous humour and in the retina. As the consequence of their presence, these dinucleotides contribute to many ocular physiological processes. On the ocular surface, dinucleoside polyphosphates can stimulate tear secretion, mucin release from goblet cells and they help epithelial wound healing by accelerating cell migration rate. These dinucleotides can also stimulate the presence of proteins known to protect the ocular surface against microorganisms, such as lysozyme and lactoferrin. One of the latest discoveries is the ability of some dinucleotides to facilitate the paracellular way on the cornea, therefore allowing the delivery of compounds, such as antiglaucomatous ones, more easily within the eye. The compound Ap 4 A has been described being abnormally elevated in patient's tears suffering of dry eye, Sjogren syndrome, congenital aniridia, or after refractive surgery, suggesting this molecule as biomarker for dry eye condition. At the intraocular level, some diadenosine polyphosphates are abnormally elevated in glaucoma patients, and this can be related to the stimulation of a P2Y 2 receptor that increases the chloride efflux and water movement in the ciliary epithelium. In the retina, the dinucleotide dCp 4 U, has been proven to be useful to help in the recovery of retinal detachments. Altogether, dinucleoside polyphosphates are a group of compounds which present relevant physiological actions but which also can perform promising therapeutic benefits.

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“…In airlifting cultures, the level of culture medium in the dish is lowered to the surface level of the epithelium, promoting stratification and differentiation of the epithelium (Ban et al, 2003, Cooper et al, 2004, Koizumi et al, 2001a, Nakamura et al, 2003b. This method can, therefore, be used for investigating the stratification and differentiation potential of cultured limbal epithelial cells with high expression of CK3 and CK12.…”

Section: Variations In Explant and Suspension Culturesmentioning

confidence: 99%

Limbal Stem Cell Transplantation and Corneal Neovascularization

Katikireddy¹,

Jurkunas²

2012

New Advances in Stem Cell Transplantation

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Comparison of ultrastructure, tight junction-related protein expression and barrier function of human corneal epithelial cells cultivated on amniotic membrane with and without air-lifting

Cited by 98 publications

References 27 publications

Factors Influencing Outcomes in Cultivated Limbal Epithelial Transplantation for Chronic Cicatricial Ocular Surface Disorders

Factors Influencing Outcomes in Cultivated Limbal Epithelial Transplantation for Chronic Cicatricial Ocular Surface Disorders

The role of dinucleoside polyphosphates on the ocular surface and other eye structures

Limbal Stem Cell Transplantation and Corneal Neovascularization

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