Comparison of Ultrasound Assisted Extraction and Conventional Extraction Technique on Recovery of Phenolic and Flavonoid Compounds from Aloe barbadensis Miller

Abstract

Aloe barbadensis Miller (Aloe vera) is useful for skin care and its bioactive compounds could replace the insecure chemical compounds that used in cosmeceutical application. This study was designed to evaluate the effect of solvents and ultrasound assisted extraction (UAE) parameters such as duty cycle and sonication time on the concentration of phenolic and flavonoid compounds from Aloe vera. The extraction efficiencies of the best condition of UAE were compared with that of conventional extraction technique. The Aloe vera gel sample was used in this study. The total phenolic and flavonoid compounds were determined by using UV-Vis spectrophotometer. The result shows that extraction solvents significantly affect extraction yield of phenolic and flavonoid compounds, and it was found that ethanol to be the most suitable solvent. The best condition of UAE was duty cycle of 50% and 40 min of sonication time. The sonication time with the UAE was 40 min, which was six-times shorter than with the conventional extraction technique. The highest concentrations of total phenolic and flavonoid compounds using UAE were found to be 11.41±0.04 and 6.76±0.05 mg/mL, respectively. The UAE was found to be more efficient in extraction of phenolic and flavonoid in comparison with conventional extraction technique.