Comparison of two bioassays, a fish liver cell line (PLHC-1) and a midge (Chironomus riparius), in monitoring freshwater sediments

Huuskonen, Sirpa; Ristola, Tiina; Tuvikene, Arvo; Hahn, Mark E.; Kukkonen, Jussi V. K.; Lindström-Seppä, Pirjo

doi:10.1016/s0166-445x(98)00070-8

Cited by 27 publications

(21 citation statements)

References 47 publications

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“…The inherent economic and ethical constraints associated with in vivo tests using live animals for cytotoxicity analysis have led to the preferred utilization of in vitro biological test systems such as cell lines derived from aquatic animal species (Keddy et al 1995;Mátlová et al 1995;Huuskonen et al 1998;Segner 1998;Olabarrieta et al 2001;Repett et al 2001;Choi and Oris 2003;Ní Shúilleabháin et al 2004;Segner 2004;Wang et al 2004;Gűlden et al 2005 ) commonly present and bioavailable in the aquatic environment. This paper describes the employment of the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and CB (Coomassie Blue) assays for the examination of cytotoxic responses of these turtle cell lines following their exposure to the four selected metal salts.…”

Section: Introductionmentioning

confidence: 99%

Validation of an in vitro cytotoxicity test for four heavy metals using cell lines derived from a green sea turtle (Chelonia mydas)

Tan

Wang

et al. 2009

Cell Biol Toxicol

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Ten cell lines established from juvenile green sea turtles were tested and evaluated for their cytotoxic responses to four heavy metals: cadmium (Cd), chromium (Cr), zinc (Zn), and copper (Cu). Following a 24-h exposure to these metal salts at selected concentrations, test cells were comparatively characterized by morphology, viability, and proliferation. Experimental results indicated that all these metal salts were cytotoxic to these turtle cell lines at varied concentrations. Calculated 10% and 50% inhibitory concentration (IC(10) and IC(50)) values revealed that the cytotoxicities of Cd and Cr were significantly more potent than the other two metal salts (p < 0.01). Comparative analysis of IC(10) values in these ten cell lines showed that turtle lung cells (GT-LG) are the most sensitive cell line to Cd, Cr, Zn, and Cu. Among these turtle cell lines, turtle liver cells (GT-LV) are more tolerant than other cells to Cd, Cr, and Zn, while GT-EYE cells are more tolerant to Cu, as determined by IC(50) values. Overall, GT-LG represents the most sensitive cells to heavy metal contamination and may be used for initial environmental monitoring, while the highly tolerant nature of GT-LV and GT-EYE cells to the tested heavy metals suggest their potential use as an emergency last-resort indicator of potential metal-related adverse effect on human health.

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Section: Introductionmentioning

confidence: 99%

Validation of an in vitro cytotoxicity test for four heavy metals using cell lines derived from a green sea turtle (Chelonia mydas)

Tan

Wang

et al. 2009

Cell Biol Toxicol

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“…These cells have been used for investigations of cytotoxicity (Babich et al 1991b;Ryan and Hightower 1994;Bruschweiler et al 1995;Huuskonen et al 1998a;Fent and Batscher 2000) and effects of AHR ligands (Bruschweiler et al I996a;Bruschweiler et al 1996b;Celander et al 1996;Celander et al 1997;Villeneuve et al 1997;Huuskonen et al 1998b;Huuskonen et al 1998c;Smeets et al 1999;Huuskonen et al 2000). PLHC-I cells show induction of CYPIA (an AHR-mediated response) in response to AHR agonists (Hahn et al 1993;Hahn et al 1996a;Hahn et al 1996b;Hestermann et al in press).…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of action for aryl hydrocarbon receptor ligands in the PLHC-1 cell line

Hestermann¹

1999

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“…Declining EROD concentration-response curves have been observed in primary cultures of rat hepatocytes (Till et al, 1999) and H4IIE cells exposed to model compounds (Xu et al, 1993;Willett et al, 1998) and environmental samples (Kopponen et al, 1994;Gale et al 2000;Huuskonen et al, 1998Huuskonen et al, , 2000. For example, Chiarolini et al (1997) observed increased EROD activity at low concentrations, but a decrease in activity at high concentrations, in Fao cells exposed to PAHs from urban air.…”

Section: Discussionmentioning

confidence: 95%

“…In such cases induction of CYP1A protein may provide a more accurate measure. In several studies, CYP1A protein content have been shown to continue to increase somewhat linearly after the decline of EROD activity (Hahn et al, 1993(Hahn et al, , 1996Brüschweiler et al, 1996;Huuskonen et al 1998). CYP1A protein content may, however, also decrease when the concentration of inducers are sufficiently high (Hahn et al, 1996;Lorenzen et al, 1997;Huuskonen et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…Because of this, CYP1A induction has been used widely in in vitro evaluation of environmental samples, e.g. in the rat hepatoma cell line H4IIE (Tillitt et al, 1991;Kopponen et al, 1994;Gale et al, 2000;Whyte et al, 2004), the mouse hepatoma cell line Hepa 1 (Kopponen et al, 1994), and the fish hepatoma cell line PLHC-1 (Huuskonen et al, 1998(Huuskonen et al, , 2000. CYP1A catalytic rate in terms of 7-ethoxyresorufin-O-deethylase (EROD) activity is the most commonly used indicator of CYP1A induction (Hahn et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of CYP1A Protein Induction, Biotransformation Capacity and DNA Adduct Formation in a Rat Hepatoma Cell Line (Fao), as Biomarkers of Organic Contamination in Environmental Soil Samples

Østby

Sundby

Krøkje

2006

Water Air Soil Pollut

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Induction of cytochrome P4501A (CYP1A) immunopositive protein was evaluated in the rat hepatoma cell line Fao, as a biomarker of organic pollution in extract of environmental soil samples, exposed to different sources and degrees of chemical contamination. Soil samples were collected in one area in Russia (Monchegorsk) and two areas in Southern Norway (Fiskaa and Birkenes). In addition, one reference soil sample was collected in Central Norway (Høylandet). Contents of selected polycyclic aromatic hydrocarbons (PAHs) in the samples were also evaluated. To further evaluate the inducibility of the most potent soil extract (Fiskaa), S9 fraction of Fao cells, pretreated with this extract, was used as an activation system in the Ames Salmonella assay. The DNA adduct forming capacity of the soil extracts, analyzed by the 32 P-postlabeling technique, was also investigated in Fao cells. The Fao cell line has previously been found to be a very sensitive biomonitoring system, that responds to environmentally relevant concentrations of planar model contaminants with increased level of CYP1A immunopositive protein and DNA adducts. In the present study the Fao cell line also showed its potential for use in evaluating the CYP1A inducing potency of environmental samples. All soil extracts induced CYP1A protein in the Fao cells, although the level of induction varied between the soil samples. The Fiskaa soil extract was the most potent CYP1A inducer and this extract also contained the highest level of PAHs. No significant correlation was observed between the level of the total of 16 PAHs and CYP1A protein level. However, a significant correlation was observed between CYP1A protein level and the level of Benzo[a]pyrene (B[a]P), which is a very potent CYP1A inducer. The S9 fraction of pretreated Fao cells activated B[a]P to mutagens in a concentration-dependent relationship, although the response was weak. No DNA adducts were detected in cells exposed to the soil extracts. This demonstrates the necessity of determining several biomarker parameters simultaneously as one single biomarker may fail to respond to potentially harmful compounds.

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Comparison of two bioassays, a fish liver cell line (PLHC-1) and a midge (Chironomus riparius), in monitoring freshwater sediments

Cited by 27 publications

References 47 publications

Validation of an in vitro cytotoxicity test for four heavy metals using cell lines derived from a green sea turtle (Chelonia mydas)

Validation of an in vitro cytotoxicity test for four heavy metals using cell lines derived from a green sea turtle (Chelonia mydas)

Mechanisms of action for aryl hydrocarbon receptor ligands in the PLHC-1 cell line

Evaluation of CYP1A Protein Induction, Biotransformation Capacity and DNA Adduct Formation in a Rat Hepatoma Cell Line (Fao), as Biomarkers of Organic Contamination in Environmental Soil Samples

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