Comparison of the therapeutic effects of bone marrow mononuclear cells and microglia for permanent cerebral ischemia

Abstract

In this study we transplanted bone marrow mononuclear cells (BM-MNCs) or microglia into rats that had undergone permanent cerebral ischemia and observed the distribution or morphology of transplanted cells in vivo. In addition, we also compared the effects of BM-MNCs and microglia on infarct volume, brain water content, and functional outcome after permanent cerebral ischemia. BM-MNCs and microglia were obtained from femur and brain, respectively, of newborn rats. Adult rats were injected with vehicle or 3 million BM-MNCs or microglia via the tail vein 24 h after permanent middle cerebral artery occlusion (pMCAO). The distribution or morphologic characteristics of transplanted BM-MNCs (BrdU/double-stained CD34 and CD45) and microglia (BrdU/double-stained Iba-1) were detected with immunofluorescent staining at 3 or 7 and 14 days after pMCAO. Functional deficits were assessed by the modified neurologic severity score at 1, 3, 7 and 14 days after pMCAO. Brain water content was assessed with dry–wet weight method at 3 days, and infarct volume was determined with 2,3,5-triphenyltetrazolium chloride (TTC) staining at 14 days. More BrdU/double-stained CD45- and Iba-1-positive cells were observed than BrdU/double-stained CD34-positive cells around the infarcted area. Some infused microglia show the morphology of innate microglia at 7 days and the increased numbers can be detected at 14 days after pMCAO. BM-MNC-treated rats showed a significant reduction in infarct volume and brain water content compared to vehicle- and microglia-treated rats. In addition, BM-MNC treatment reduced neurologic deficit scores compared to those in the other groups. The results provide evidence that infusion of BM-MNC, but not microglia, is neuroprotective after permanent cerebral ischemia.

Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats

“…The pro-angiogenic potentials of BM-MNCs and BMSCs have been demonstrated not only in skin flap survival model but also in the other ischemic diseases [7,8,[13][14][15][16][17]. However, which is the optimal cell source for clinical application is not clear.…”

Wharton’s Jelly Transplantation Improves Neurologic Function in a Rat Model of Traumatic Brain Injury

“…On days 1, 3, 7, 14, 21, and 28 after TBI, neurologic deficit was evaluated with the modified neurologic severity score (mNSS), which measures motor, sensory, reflex, and balance deficits (Zhang et al 2009; Jiang et al 2013). The mNSS test is graded on a scale of 0 (normal) to 18 (maximal deficit).…”

Structural and ultrastructural evaluation of the aortic wall after transplantation of bone marrow-derived cells (BMCs) in a model for atherosclerosis

“…C57BL/6 was anaesthetized with pentobarbital (150 mg/kg), and Pagination not final (cite DOI) / Pagination provisoire (citer le DOI) BMCs were isolated from the tibias and femurs Jiang et al 2013). The medullar cavities of the bones were exposed and flushed with DMEM (Dulbecco's Modified Eagle's Medium, Sigma-Aldrich, St. Louis, Missouri, USA), pH 7.2 .…”

“…Studies have shown that endothelial progenitor cells can repair and renovate some specific arterial physiological conditions. For example, progenitor cells stabilize atherosclerotic plaque in pathological conditions (Xu 2008), and studies have shown that the infusion of BMCs is neuroprotective after permanent brain ischemia (Jiang et al 2013). Moreover, other studies have shown that endothelial progenitor cells contribute to the formation of atherosclerotic lesions (Fadini et al 2007).…”