Comparison of the sperm aneuploidy rate in severe oligozoospermic and oligozoospermic men and its relation to intracytoplasmic sperm injection outcome

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Purpose To investigate the association of Progesterone Receptor (PR) gene variations and male infertility Methods DNA extraction, PCR and sequencing of PR gene, PROGINS insertion by PCR. Association of the variations with seminal parameters and outcomes of ICSI. Results Four known SNPs in the PR gene were identified in the study of which three (rs3740753, rs1042838, rs104283) were co-inherited and in complete linkage disequilibrium with the PROGINS Alu insertion. There were no differences in their frequencies between fertile and infertile males. The rs2020880 was found at a very low frequency only in the controls but not in the infertile subjects. The sperm counts, fertilization rate, embryo quality or pregnancy rates were not different in individuals with or without PROGINS allele. Conclusion PR gene alterations are not associated with male infertility or ICSI outcome.

Section: Discussionsupporting

confidence: 58%

“…All the subjects (irrespective of sperm counts) underwent ICSI as per the standard protocol at INKUS IVF Centre. The protocol for ovarian hyperstimulation, oocyte retrieval, ICSI and embryo culture has been detailed elsewhere [9,29].…”

Section: Methodsmentioning

confidence: 99%

Association of progesterone receptor gene polymorphism with male infertility and clinical outcome of ICSI

Sen

Dixit

Thakur

et al. 2013

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“…Standard semen analysis is routinely used to evaluate the male infertility; however, some researchers believe that sperm functional test should also be used with standard semen analysis for evaluation of male fertility. The success rate of fertilization is greatly affected by the quality of sperm used for insemination [18,19].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion

Kazerooni

Asadi

Jadid

et al. 2009

103

Objective To evaluate the sperm's chromatin quality in couples with spontaneous recurrent abortion. Methods Thirty couples with spontaneous recurrent abortion (case group) and 30 fertile couples (control group) referring to Zeinabieh Gynecology clinic of Shiraz were included. Semen samples were collected for each participant and were used for standard semen analysis and sperm nuclear maturity tests including Chromomycin A3 (CMA3), Aniline Blue (AB) staining and Acridine Orange (AO) test (by light microscopy).Result Patients in case group had significantly higher percentage of CMA3 (p < 0.001) and AB (p <0.001) positive spermatozoa compared to controls. However AO results did not differ significantly between groups (p= 0.656). Sperm morphology and progressive motility were negatively correlated with CMA3 (p=0.001 and p=0.043) and AB (p=0.015 and p=0.031) respectively. Conclusion Evaluation of the sperm's quality via CMA3 and AB staining could be considered as one of the complementary tests of semen analysis for assessment of male factor in couples with spontaneous recurrent abortion.

“…Ovarian stimulation and ICSI procedures was performed by skilled and experienced person and as per the standard protocol performed at INKUS IVF Centre (Mumbai, India) which has been detailed elsewhere [26] A total of 104 cycles were evaluated for fertilization rate, embryo quality and pregnancy rate. Only good quality oocytes, as per published standards (Metaphase II and had the first polar body extruded) were considered for microinjection [48].…”

Section: Assessment Of Fertilization and Embryo Qualitymentioning

confidence: 99%

Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate

Bhilawadikar

Zaveri²,

Mukadam³

et al. 2013

Self Cite

Purpose To compare the expression profiles of Tektin 2 and CatSper 2 motility proteins in the spermatozoa of normozoospermic and oligoasthenozoospermic men and determine its correlation with sperm motility, fertilization rate, embryo quality and pregnancy rate. Methods Tektin 2 and CatSper 2 protein expression was studied using Western Blotting and immunofluorescence. Tektin 2 and CatSper 2 protein levels were quantified by ELISA. Results Oligoasthenozoospermic men were found to have lower fertilization rates, poor embryo quality and lower pregnancy rates as compared to normozoospermic men.The levels of Tektin 2 and CatSper 2 are significantly lower in spermatozoa of oligoasthenozoospermic men as compared to normozoospermic controls; the levels were also lower in immotile fraction as compared to motile fraction of spermatozoa obtained from normozoospermic individuals. The levels of Tektin 2 and CatSper 2 were higher in individuals demonstrating sperm motility >60 % as compared to sperm motility <30 %. Tektin 2 but not CatSper 2 levels were positively associated with fertilization rate, embryo quality and pregnancy rate. Conclusion Levels of Tektin 2 and CatSper 2 proteins are positively associated with sperm motility parameters. Measurements of Tektin 2 levels can be correlated with the clinical outcome of ICSI.