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Cited by 58 publications
(41 citation statements)
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References 46 publications
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“…One of the most important properties of the immobilized enzyme, primarily due to its reusability, is the prerequisite that commercialization of biocatalysts demands that the enzymatic process be economically cost saving; this makes it superior to the free counterpart by assembling an enzymatic process that is economically favourable and deeply rooted in the field of biotechnology [63,64]. To find out how reusable the enzyme is, the spectrophotometric assay was conducted using varying concentrations of neostigmine methyl sulfate and half-maximal inhibitory con- Figure 8 The IC 50 values The IC 50 (μg/ml) values of free enzyme and porous silicon-immobilized acetylcholinesterase after repeated use for up to three cycles.…”
Section: Reusabilitymentioning
confidence: 99%
“…One of the most important properties of the immobilized enzyme, primarily due to its reusability, is the prerequisite that commercialization of biocatalysts demands that the enzymatic process be economically cost saving; this makes it superior to the free counterpart by assembling an enzymatic process that is economically favourable and deeply rooted in the field of biotechnology [63,64]. To find out how reusable the enzyme is, the spectrophotometric assay was conducted using varying concentrations of neostigmine methyl sulfate and half-maximal inhibitory con- Figure 8 The IC 50 values The IC 50 (μg/ml) values of free enzyme and porous silicon-immobilized acetylcholinesterase after repeated use for up to three cycles.…”
Section: Reusabilitymentioning
confidence: 99%
“…Isso mostra que o presente estudo apresenta conversões bastante satisfatórias usando o alginato de cálcio tamponado sem variar as razões molares dos reagentes. Além disso, os bons resultados de esterificação do valerato de etila podem ser devido ao fato das reações ocorrerem em meios contendo n-heptano que mostra ser um solvente bastante adequado, auxiliando a produção do valerato de etila na fase orgânica (Martins et al, 2014;Guillén et al, 2012). Haja vista que muito dos estudos encontrados na literatura com biocatalisadores usa n-heptano na conversão dos ésteres.…”
Section: Efeito Do Ph Na Esterificaçãounclassified
“…O principal propósito de imobilizar uma enzima é obter um biocatalisador com atividade e estabilidade, que não sejam afetados durante o processo, o que não ocorre com as enzimas na forma livre (Inanov e Scheneider, 1997;Dave e Madamwar, 2006;Bayramoğlu et al, 2011). Embora as reações de esterificação sejam comuns, os biocatalisadores ainda precisam ser compreendidos em relação ao seu comportamento e especificidade na síntese de ésteres aromáticos (Martins et al, 2014). Dessa forma, ainda existe um grande campo a ser explorado, relacionado à síntese enzimática de ésteres aromáticos em sistemas que empreguem materiais de baixo custo como suportes, gerando resultados que permitam verificar a viabilidade técnica e econômica da produção desses compostos.…”
Section: Introductionunclassified
“…The conventional method for optimization of process parameters involves changing one variable at a time, while keeping others at fixed levels. [39][40][41][42][43][44] Commercial technologies based on immobilized enzymes are based on batch operations in stirred tank reactors. These investigations can clarify the effect of individual variables on the desired output such as conversion and selectivity, but may not really account for the interactive effects between the variables.…”
Section: Introductionmentioning
confidence: 99%