Comparison of the performance of different reversed‐phase columns for liquid chromatography separation of 11 pollutant phenols

Abstract

A systematic optimization of the HPLC separation of a mixture containing 11 pollutant phenols (PPs) using a Hypersil ODS (250 mm64.6 mm, 5 lm) column and UV-DAD detection has been carried out. The binary mobile phases used were obtained by mixing 50 mM phosphate (pH = 3.0) and methanol, ACN, or THF as organic modifiers. After selecting ACN as an organic modifier, the effects of pH and temperature on PPs separation were studied. A mobile phase of 50 mM acetate (pH = 5.0) -ACN (60 : 40 v/v) at 508C allowed the separation of 11 phenols but not to baseline in 17 min. To improve the performance of this separation, the following RP columns were tested: Luna C18 (2), Purospher C18, Synergi C12, Synergi Fusion C18, Gemini C18, Luna Cyano, Lichrospher C8, and Envirosep-PP (polymeric). In all the cases, the performance (analysis time, retention, selectivity, resolution, asymmetry factors, and efficiency) was evaluated. A further reoptimization of the mobile phase was carried out for all the columns by studying the ACN content and pH, with the aim of improving the above-mentioned separations and selecting the most suitable one for PPs analysis.

Determination of priority phenolic pollutants exploiting an in-syringe dispersive liquid–liquid microextraction–multisyringe chromatography system

“…Phenolic compounds have been determined using different strategies, mainly exploiting separation techniques, e.g., gas chromatography and liquid chromatography [12,13]. Liquid chromatography is one of the most used techniques since derivatization of analytes is avoided [13,14].…”

“…Liquid chromatography is one of the most used techniques since derivatization of analytes is avoided [13,14]. Moreover, the advent of monolithic columns made possible the development of low-pressure chromatographic techniques such as sequential injection chromatography [15] and multisyringe chromatography (MSC) [16] as a result of the implementation of monolithic columns in sequential injection analysis (SIA) and multisyringe flow injection analysis (MSFIA) [17] systems, respectively.…”

A Facile Profiling Method of Short Chain Fatty Acids Using Liquid Chromatography-Mass Spectrometry

“…LC run time was chosen in our study. Alternatively, C8 reversed-phase column may perform similarly as C18 reversed-phase column in a shorter run time, although a significant effort needs to optimize chromatographic condition [24,25]. Various kinds of metabolites in biological samples often contain carboxylic acids.…”

18‐crown‐6 as a class I organic modifier in MEKC

“…NT and NP are of intense environmental and forensic interest 42–44. Both isomer series (which include ortho , meta , and para members) are well characterized 43, 45–49, with NT previously being used to examine class I and II modifiers in VEKC 9.…”