Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs

Abstract

BackgroundThe aim of the present study was to compare the osteogenic potential of mesenchymal stem cells extracted from the bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of young dogs. The following parameters were assessed: dimethyl thiazolyl diphenyl tetrazolium (MTT) conversion, alkaline phosphatase (ALP) activity, collagen and mineralised matrix synthesis, and the expressions of osterix, bone sialoprotein (BSP), and osteocalcin (OC).ResultsMTT conversion was greater in BM-MSCs compared to AD-MSCs after 14 and 21 days of differentiation; ALP activity was greater in differentiated AD-MSCs on day 7; collagen synthesis was greater in BM-MSCs on days 14 and 21; the percentage of mineralized area per field was greater in BM-MSCs compared to AD-MSCs; osterix expression was greater in BM-MSCs in days 14 and 21, and BSP and OC expression levels were greater in BM-MSCs at all the investigation time-points.ConclusionsIt was concluded that the osteogenic potential was greater in BM-MSCs than AD-MSCs when extracted from young dogs.

Different Bone Healing Effects of Undifferentiated and Osteogenic Differentiated Mesenchymal Stromal Cell Sheets in Canine Radial Fracture Model

“…Mesenchymal stromal cells (MSCs) have potentials for bone regeneration [1][2][3]. Previous reports indicated that MSCs could repair damaged tissue by replacing damaged cells [3].…”

Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs

“…To maximize osteogenic differentiation, in vitro differentiation is recommended before clinical use (LIU et al, 2013), as was performed in the present study (Figure 1). The formation of mineralized nodules and increases in expression of OSX, BSP, and OC (Figure 1) are considered reliable markers of osteogenic differentiation (ALVES et al, 2014;ALVES et al, 2015a).…”

“…For analgesia, meloxicam (Maxicam, Ouro Fino, Brazil) (0.2mg kg -1 IM) was used every 24 hours for three days. Adipose tissue (1cm 3 of each animal) was collected from the subcutaneous gluteal region and processed according to ALVES et al (2014). Briefly, the samples were washed with 0.15M PBS and digested in 20mL of 0.1% w/v collagenase B solution (Roche, Germany), in a 5% CO 2 incubator at 37°C for 45 minutes.…”

Sustained knock down of PPARγ and bFGF presentation in collagen hydrogels promote MSC osteogenesis

“…For assaying the effect of bFGF on cell proliferation within collagen hydrogel, 1 × 10 4 MSCs were incorporated into 500 μL hydrogels and poured onto 24-well plates. At different time points during the cultivation, cells were collected by digesting collagen with type I collagenase (10 mg/mL).…”

“…For serious bone defects, it is usually difficult to heal through endogenous regenerative mechanisms, especially for aged patients. Extrinsic regenerative strategies, therefore, attract more and more attention from clinicians and investigators [3,4].…”