Comparison of the Mycobacterium Growth Indicator Tube Method and the Method of Proportion for Drug Susceptibility Testing of Mycobacterium Tuberculosis

Aktaş, Ayşe; Yiğit, Nimet; Ayyıldız, Ahmet; Baştopçu, Ayşe

doi:10.5152/eajm.2014.23

Cited by 6 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sub-culturing was performed for 112 LJ-culture positive freezed isolates using BACTEC MGIT 960 instrument (Becton Dickinson, Baltimore, MD, USA) for a maximum of 42 days from initial incubation date.. The drug sensitivity test to streptomycin (S), isoniazid (I), rifampicin (R) and ethambutol (E) was done for all of the successfully recovered sub-culture positive isolates following the protocols [ 14 ]. To control tubes, 0.5 ml of Growth Control working solution was added and labeled as drug free Mycobacterium growth indicator tubes (MGIT), and the other drugs containing tubes were labeled S, I, R and E. The result was interpreted as the growth unit (GU) of the growth control (GC) reached at a minimum value of 400.…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and genotypic drug sensitivity profiles of Mycobacterium tuberculosis infection and associated factors in northeastern Ethiopia

et al. 2021

View full text Add to dashboard Cite

Background Tuberculosis is a devastating and a deadly disease despite the novel advances in its diagnostic tools and drug therapy. Drug resistant Mycobacterium contributes a great share to tuberculosis mortality. Status of drug resistance and patients’ awareness toward the disease is unknown in northeastern Ethiopia. Thus, the aim of this study was to determine the phenotypic and genotypic drug sensitivity patterns and associated factors in Oromia Special Zone and Dessie Town, northeastern Ethiopia. Methods In a cross-sectional study, 384 smear positive tuberculosis cases were recruited and Löwenstein-Jensen culture was done. The performance of GenoTypic MTBDRplus assay using the conventional BACTEC MGIT 960 as a “gold standard” was determined. Drug resistant strains were identified using spoligotyping. Pearson Chi-square test was used to determine the association of drug sensitivity test and tuberculosis type, lineages, dominant strains and clustering of the isolates. Results The 384 smear positive Mycobacterium samples were cultured on LJ media of which 29.2% (112/384) as culture positive. A fair agreement was found between MTBDRplus assay and the conventional MGIT test in detecting the Mycobacterium tuberculosis with sensitivity, specificity, positive and negative predictive value of 94.2, 30.2, 68.4 and 76.5%, respectively. Among LJ culture positive samples 95 of them gave valid result for MTBDRplus assay and 16.8% (16/95) as drug resistant. Similarly, MGIT subculture was made for the 112 isolates and 69 of them gave positive result with 15.9% (11/69) as drug resistant. Cohen’s kappa value showed almost a perfect agreement between the two testing methods in detecting rifampicin (sensitivity 100% and specificity 98.3%) and multi-drug resistance (sensitivity 83.3% and specificity 100%). Spoligotyping identified 76.5% (13/17) of the drug resistant isolates as Euro-American and family 33 as the predominant family. Significant association was observed between drug resistant isolates and the dominant strains (χ2: 34.861; p = 0.040) of the Mycobacterium. Conclusion Higher magnitude of drug resistance was found in the study area. The GenoTypic MDRTBplus assay had an acceptable drug sensitivity testing performance.

show abstract

Section: Methodsmentioning

confidence: 99%

Phenotypic and genotypic drug sensitivity profiles of Mycobacterium tuberculosis infection and associated factors in northeastern Ethiopia

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Each isolate was tested for susceptibility to four first-line anti-tuberculosis agents: isoniazid (0.2 and 0.4 μg/ml), rifampicin (40 and 80 μg/ml), streptomycin (4 and 8 μg/ml) and ethambutol (2 and 4 μg/ml) using Method of Proportion (MOP) on L-J medium [ 12 ]. They all proved susceptible to all tested medicines.…”

Section: Case Presentationmentioning

confidence: 99%

Intracardiac tuberculomas caused by Mycobacterium tuberculosis in a dog

Szaluś‐Jordanow

Augustynowicz–Kopeć²,

Czopowicz

et al. 2016

BMC Vet Res

View full text Add to dashboard Cite

BackgroundThis paper presents an unusual form of disseminated Mycobacterium tuberculosis infection in a dog. The infection lasted at least one year and its main gross lesions were massive cardiac tuberculomas. To the best of our knowledge, this is the first report of heart tuberculomas in a dog.Case presentationA 9-year-old mixed-breed male dog weighing 10 kg was referred to the clinic for cardiological evaluation before general anesthesia. The echocardiography revealed a lump of about 20 mm in diameter in the area of the left atrium. Almost one year later the same dog was presented again in severe clinical state (fever, anorexia, weight loss, depression, cough, dyspnea, lymphadenomegaly, vomiting, recent episodes of fainting). Due to progression of the disease and poor effects of treatment the owner decided to euthanize the dog. Most prominent lesions observed during autopsy were diffuse pneumonia, fibrinous pericarditis and epicarditis as well as large, yellow, semisolid masses of caseous necrosis in the left and right atrium (30 mm and 15 mm in diameter, respectively). From both pulmonary and cardiac lesions M. tuberculosis was isolated on Lowenstein-Jensen slants and in Bactec Mycobacteria Growth Indicator Tube 960 liquid media, and confirmed by BD ProbeTec ET Direct Detection Assay and spoligotyping.ConclusionCompanion animals may occasionally suffer from tuberculosis but majority of cases probably remain misdiagnosed or undetected. Typically tuberculosis in dogs affects lungs and their regional lymph nodes. Even in humans tuberculomas are rare manifestation of mycobacterial infection, mostly seen in the central nervous system. Atypical location of main tuberculous lesions may account for lack of correct ante mortem diagnosis in this case.

show abstract

“…[16] In the studies on MDR tuberculosis worldwide, quite different MDR-Mycobacterium tuberculosis complex strain resistance ratios have been reported. In Japan Hattori et al [20] reported a very low ratio of 0.2% in their study; in Indonesia, Lisdawati et al [21] reported MDR-M. tuberculosis complex strain rate as 5.4%, in their study; in Ethiopia, Solomon et al [22] reported a MDR rate of 2.27% in society, while the MDR rate for inmates living in prison was 15.89%; in China, He et al [15] reported a MDR-M. tuberculosis complex strain ratio of 6.2%. In a well-attended study; while in Pakistan Akhtar et al [23] reported a quite high resistance ratio of 69% in their study.…”

Section: Discussionmentioning

confidence: 99%

Türkiyenin Doğusunda Çeşitli Klinik Örneklerden İzole Edilen Mycobacterium tuberculosis Suşlarinin Major Anti-Tüberküloz İlaçlara Duyarlılıklarının Araştırılması

Yilmaz

Afşin

2021

Journal of Contemporary Medicine

View full text Add to dashboard Cite

In the study, 419 Mycobacterium tuberculosis complex strains, which were isolated from samples sent to the Regional Tuberculosis Laboratory in our city between 2015 and 2019 with suspected tuberculosis from Erzurum and surrounding cities were investigated. Material and Method: Our research is a retrospective crosssectional study and clinical samples sent to the Regional Public Health Laboratory with suspicion of Tuberculosis within a five-year period from January 2015 to December 2019 were first evaluated by using Ehrlich-Ziehl-Neelsen staining technique. The samples were then simultaneously inoculated into the automated culture system (BACTEC MGIT 960) with Löwenstein-Jensen medium. Sensitivity research was conducted for isoniazide (INH), rifampicin (RIF), streptomycin (STM) and ethambutol (ETM) antibiotics with BACTEC MGIT 960 system to samples defined by M. tuberculosis complex strains identification test (TBc ID) from reproduction positive tubes. Results: In our study, 337 (80.4%) of the 419 M. tuberculosis complex strains investigated in our study were susceptible to all major anti-TB drugs, while 82 (19.6%) were found to be resistant to at least one major anti-TB drug. The distribution of resistance ratios was INH (11.9%), STM (11.7%), RIF (4.1%), ETM (3.6%). Multi-drug resistant (MDR)-TB ratio (IZN+ RIF) was found to be (3.6%). Conclusions: In our study, in the strains in which resistance to anti-TB drugs was investigated, the highest drug resistance was determined for IZN. The lowest drug resistance was determined for ETM.

show abstract

Comparison of the Mycobacterium Growth Indicator Tube Method and the Method of Proportion for Drug Susceptibility Testing of Mycobacterium Tuberculosis

Cited by 6 publications

References 14 publications

Phenotypic and genotypic drug sensitivity profiles of Mycobacterium tuberculosis infection and associated factors in northeastern Ethiopia

Phenotypic and genotypic drug sensitivity profiles of Mycobacterium tuberculosis infection and associated factors in northeastern Ethiopia

Intracardiac tuberculomas caused by Mycobacterium tuberculosis in a dog

Türkiyenin Doğusunda Çeşitli Klinik Örneklerden İzole Edilen Mycobacterium tuberculosis Suşlarinin Major Anti-Tüberküloz İlaçlara Duyarlılıklarının Araştırılması

Contact Info

Product

Resources

About