Comparison of the metabolite profile of donkey and horse seminal plasma and its relationship with sperm viability and motility

Catalán, Jaime; Yánez-Ortiz, Iván; Martínez-Rodero, Iris; Mateo-Otero, Yentel; Nolis, Pau; Yeste, Marc; Miró, Jordi

doi:10.1016/j.rvsc.2023.105046

Cited by 2 publications

(2 citation statements)

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“…Moreover, this study found higher concentrations of metabolites like citrate and lactate in the seminal plasma of stallions with a low first cycle fertility rate, as previously observed in other species, including humans, indicating that these metabolites could be observed as possible biomarkers of fertility also in stallions. The concentration of citrate in seminal plasma also resulted in being negatively correlated with the curvilinear velocity (VCL) and average path velocity (VAP) of sperm in a recent paper by Catalàn and colleagues [53]. These authors applied 1 H-NMR to the seminal plasma of horses and donkeys, showing that the qualitative profile remains consistent across these two species, with 28 common metabolites identified.…”

Section: Pony Mares *mentioning

confidence: 93%

“…Studies have been performed on seminal plasma in humans [58][59][60] and in different animal species [61][62][63][64]. Three of them [51][52][53] have focused on equine seminal plasma, providing interesting insights into the metabolites contained in this biofluid. A preliminary study performed by Magistrini et al [51] identified, by 1 H-NMR, metabolites defined as markers of sex gland secretions.…”

Section: Pony Mares *mentioning

confidence: 99%

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Nuclear Magnetic Resonance (NMR) Metabolomics: Current Applications in Equine Health Assessment

Laus,

Bazzano,

Spaterna

et al. 2024

Metabolites

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Metabolomics can allow for the comprehensive identification of metabolites within biological systems, at given time points, in physiological and pathological conditions. In the last few years, metabolomic analysis has gained popularity both in human and in veterinary medicine, showing great potential for novel applications in clinical activity. The aim of applying metabolomics in clinical practice is understanding the mechanisms underlying pathological conditions and the influence of certain stimuli (i.e., drugs, nutrition, exercise) on body systems, in the attempt of identifying biomarkers that can help in the diagnosis of diseases. Proton Nuclear Magnetic Resonance spectroscopy (1H-NMR) is well tailored to be used as an analytical platform for metabolites’ detection at the base of metabolomics studies, due to minimal sample preparation and high reproducibility. In this mini-review article, the scientific production of NMR metabolomic applications to equine medicine is examined. The research works are very different in methodology and difficult to compare. Studies are mainly focused on exercise, reproduction, and nutrition, other than respiratory and musculoskeletal diseases. The available information on this topic is still scant, but a greater collection of data could allow researchers to define new reliable markers to be used in clinical practice for diagnostic and therapeutical purposes.

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Section: Pony Mares *mentioning

confidence: 93%

Section: Pony Mares *mentioning

confidence: 99%

Nuclear Magnetic Resonance (NMR) Metabolomics: Current Applications in Equine Health Assessment

Laus,

Bazzano,

Spaterna

et al. 2024

Metabolites

View full text Add to dashboard Cite

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Comparative Study on the Sperm Proteomes of Horses and Donkeys

Ren,

Wen,

et al. 2024

Animals

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The reproductive performance of horse sperm and donkey sperm has been reported to differ. Sperm proteins play a crucial role in sperm viability and fertility. Although differences between species are known, no prior study has investigated disparities in the sperm proteome between horses and donkeys. Therefore, this study characterized and compared the sperm proteomes of horses and donkeys using 4D-DIA mass spectrometry technology. We identified 3436 proteins in horse sperm and 3404 proteins in donkey sperm. Of these, 3363 proteins were expressed in both horse and donkey sperm, with 73 proteins being specifically expressed in horse sperm, and 41 in donkey sperm. According to data analysis, donkeys exhibited a greater percentage of motility and progressive movement in straight-line sperm than horses, as well as lower percentages of static and slow sperm than horses. Joint analysis of the results from the horse and donkey sperm proteomes and their CEROS II-read parameters demonstrated a possible association between sperm proteins and their sperm viability patterns. These findings suggest that there are discrepancies in the expression levels and protein compositions of horse and donkey sperm and that certain specific proteins may be responsible for the differences in performance between these two species.

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Comparison of the metabolite profile of donkey and horse seminal plasma and its relationship with sperm viability and motility

Cited by 2 publications

References 72 publications

Nuclear Magnetic Resonance (NMR) Metabolomics: Current Applications in Equine Health Assessment

Nuclear Magnetic Resonance (NMR) Metabolomics: Current Applications in Equine Health Assessment

Comparative Study on the Sperm Proteomes of Horses and Donkeys

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