Comparison of the Effect of ELF on c‐myc Oncogene Expression in Normal and Transformed Human Cells

Czerska, Ewa M.; Casamento, Jon P.; Ning, John; Swicord, Mays L.; Al-Barazi, Heba; Davis, Christophér C.; Elson, Edward C.

doi:10.1111/j.1749-6632.1992.tb49624.x

Cited by 18 publications

(12 citation statements)

References 5 publications

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“…In the case of transformation experiments, we considered animal studies that suggest a tendency toward a dose response in terms of MF flux density and exposure duration (3)(4)(5)(6) (42,43), have indicated that a brief exposure to a magnetic field is enough to evoke the same level of effect as a prolonged exposure. In other studies, no differences in gene responses were observed in several extremely low frequencies (up to 100 Hz) and MF flux densities (32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43). For our present work involving gene expression in HL60 cells, we selected nine 60-Hz MF exposure parameters tested by Goodman et al (34).…”

Section: Discussionmentioning

confidence: 98%

“…Because our working hypothesis is based on changes in gene expression, we needed to address contradictory reports concerning the ability of MFs to increase levels of a number of gene transcripts (32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43). Our second series of studies was designed to confirm with solid data whether MFs induce significant changes in transcription of seven genes.…”

mentioning

confidence: 99%

“…The ribosomal cDNA probes for the 5-kb 28S rRNA and 1.9-kb 18S rRNA were generated from total RNA using SuperScript RNase-H Reverse Transcriptase (Gibco BRL (12)(13)(14)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43).…”

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confidence: 99%

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Rodent cell transformation and immediate early gene expression following 60-Hz magnetic field exposure.

Balcer‐Kubiczek

Zhang

Harrison

et al. 1996

Environ Health Perspect

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Some epidemiological studies suggest that exposure to power frequency magnetic fields (MFs) may be associated with an elevated risk of human cancer, but the experimental database remains limited and controversial. We investigated the hypothesis that 60-Hz MF action at the cellular level produces changes in gene expression that can result in neoplastic transformation. Twenty-four hour 200 microT continuous MF exposure produced negative results in two standard transformation systems (Syrian hamster embryo cells and C3H/10T1/2 murine fibroblasts) with or without postexposure to a chemical promoter. This prompted a reexamination of previously reported MF-induced changes in gene expression in human HL60 cells. Extensive testing using both coded and uncoded analyses was negative for an MF effect. Using the same exposure conditions as in the transformation studies, no MF-induced changes in ornithine decarboxylase expression were observed in C3H/10T1/2 cells, casting doubt on a promotional role of MF for the tested cells and experimental conditions. Images Figure 1. Figure 2. A Figure 2. B Figure 2. C Figure 2. D Figure 3. A Figure 3. B Figure 4. Figure 5. A Figure 5. B Figure 5. C Figure 5. D Figure 5. E Figure 6. A Figure 6. B Figure 6. C Figure 6. D Figure 6. E Figure 7. Figure 8. A Figure 8. B Figure 8. C Figure 9. Figure 10. A Figure 10. B

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

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Rodent cell transformation and immediate early gene expression following 60-Hz magnetic field exposure.

Balcer‐Kubiczek

Zhang

Harrison

et al. 1996

Environ Health Perspect

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“…Despite many studies that have been conducted to investigate biological effects of ELF-MFs, there remains considerable controversy concerning whether reported biological effects would lead to a health hazard. Earlier studies reported that ELF±MFs can affect gene expression, particularly at the transcriptional level in human leukemia promyelocytic HL-60 cells, human lymphocytic Daudi cells, and T-lymphoblastoid CEM CE3 cells [Goodman et al, 1989;Czerska et al, 1992;Phillips, 1993]. In other reports, however, no clear positive results on induction of expression have been presented [Lacy Hulbert et al, 1995;Miyakoshi et al, 1996;Saffer and Thurston, 1995;Owen, 1998].…”

Section: Introductionmentioning

confidence: 96%

Elliptically polarized magnetic fields do not alter immediate early response genes expression levels in human glioblastoma cells

Yomori

Yasunaga

Takahashi

et al. 2002

Bioelectromagnetics

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Expression of immediate early response genes such as c-fos, c-jun, and c-myc in response to 1-500 microT resultant (r) 60 Hz elliptically polarized (EP) magnetic fields (MFs), typical of environmental MFs polarization under overhead power lines, was analyzed in both at transcriptional and translational levels using human glioblastoma (T98G) cells. Pseudo synchronized T98G cells at G1 phase were exposed to EP-MFs (1, 20, 100, and 500 microTr) for up to 3 h, but produced no statistical difference (P>0.05) in the levels of expression ratio at both the transcriptional and translational levels at 30 min for c-fos and c-jun and at 180 min for c-myc after serum stimulation. In addition, exposure of T98G cells to linearly (vertical and horizontal) and/or circularly polarized MFs (500 microTr) produced no significant change (P>0.05) in the expression ratio at both transcriptional and post-transcriptional levels. Thus, there was no evidence that linearly or rotating polarized MFs enhanced early response gene expression in these studies. These results suggest that environmental MFs at 1-500 microT flux density are unlikely to induce carcinogenesis through a mechanism involving altered expression of the immediate early response genes.

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“…Electromagnetic fields (EMF) are produced when electric current flows through an electrical conductor like power line [18]. Like MF and EF, EMF also has biological effects such as altered rate of cell growth [5], [19], altered quantities of RNA transcript and proteins [20], altered cell surface properties [21] and effect on development [22]. However EMFbased technologies have not progressed to clinical translation and the reason for this is the scepticism due to differences in experimental exposure protocols and static MF (SMF) variation applied in experiment [23].…”

Section: Introductionmentioning

confidence: 99%

A Preliminary Study on Magnetic Fields Effects on Stem Cell Differentiation

Miskon

Uslama

2011

IFMBE Proceedings

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Abstract-The objective of this paper is to provide the fundamental mathematical formula to predict the effect of magnetic fields (MF) on stem cell differentiation. The data were reviewed from journals related to the effects of magnetic fields on stem cell differentiation. These data were given a value for differentiation which is related to their MF strength with these conditions; MF strength that does not affect the stem cell differentiation given the value of zero, MF strength that results in stem cells death given the value of 1, and the MF strength that affects stem cells The differentiation given the value between 0.1 and 0.9. graph was plotted according to these data and the mathematical equation is designed from the graph. From this review, we suggest that the intensity of MF that can affect the stem cell differentiation is between 600µT and 9.4T in which the cell differentiation will not occur with intensity of less than 10µT and intensity of more than 12T will cause the death of stem cells. We also suggest that the limit of MF effects on stem cell differentiation lies between 10 µT and 600 µT, and the limit of MF strength that can lead to the death of stem cells lies between 9.4 T and 12 T. It can be concluded that the exposure of MF on stem cell differentiation depends not only on the MF intensity, but also on the period of exposure.

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Comparison of the Effect of ELF on c‐myc Oncogene Expression in Normal and Transformed Human Cells

Cited by 18 publications

References 5 publications

Rodent cell transformation and immediate early gene expression following 60-Hz magnetic field exposure.

Rodent cell transformation and immediate early gene expression following 60-Hz magnetic field exposure.

Elliptically polarized magnetic fields do not alter immediate early response genes expression levels in human glioblastoma cells

A Preliminary Study on Magnetic Fields Effects on Stem Cell Differentiation

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