Comparison of the detection of Helicobacter pylori infection by commercially available serological testing kits and the 13C-urea breath test

Kawai, Shinya; Arai, Kohei; Lin, Yingsong; Nishiyama, Takeshi; Sasakabe, Tae; Wang, Chaochen; Miwa, Hiroto; Kikuchi, Shogo

doi:10.1016/j.jiac.2019.03.026

Cited by 13 publications

(17 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detection of serum IgG against H. pylori is usually based on the enzyme-linked immunosorbent assays (ELISA). The latex immunoassay (Eiken Chemical Co, Ltd., or Denka Seiken Co, Ltd., Tokyo, Japan), is also employed with some advantage in terms of time consumed [ 104 ]. Several kits are available on the market and, overall, they are highly sensitive and specific.…”

Section: Non-invasive Testsmentioning

confidence: 99%

What Is New in Helicobacter pylori Diagnosis. An Overview

Dore

2021

JCM

View full text Add to dashboard Cite

Helicobacter pylori infection remains one of the most prevalent infections worldwide, especially in low-resource countries, and the major risk factor for peptic ulcer and gastric cancer. The “test-and-treat” strategy is recommended by several guidelines and consensus. The choice of testing method is based on patient age, presence of alarm signs and/or symptoms, use of non-steroidal anti-inflammatory drugs, as well as local availability, test reliability, and cost. Culture is the gold standard to detect H. pylori and, possibly, to perform susceptibility testing, however, it requires upper endoscopy and dedicated labs. Recent advances in molecular biology have provided new strategies in detecting infection and antimicrobial resistance without invasive tests. In this review we attempt to offer a comprehensive panorama on the new diagnostic tools and their potential use in clinical settings, in order to accomplish specific recommendations.

show abstract

Section: Non-invasive Testsmentioning

confidence: 99%

What Is New in Helicobacter pylori Diagnosis. An Overview

Dore

2021

JCM

View full text Add to dashboard Cite

show abstract

“…In previous studies, HP infection was detected with a serology test by enzyme-linked immunosorbent assay (ELISA) and confirmed with western blotting (WB) [6,7,9,[16][17][18], whereas in the present study, 13 C-and 14 C-UBT were used to detect HP infection. UBT is the best noninvasive method for patients without gastric resection or proton pump inhibitor (PPI) treatment, with both high positive predictive value and negative predictive value [19][20][21]. A meta-analysis suggested that UBT had high diagnostic accuracy for detecting HP infection in patients with dyspepsia, with a pooled sensitivity of UBT in adult patients of 96% and a pooled specificity of 93% [22,23].…”

Section: Discussionmentioning

confidence: 99%

Sperm parameters and anti-Müllerian hormone remain stable with Helicobacter pylori infection: a cross-sectional study

Feng

Huang

et al. 2020

BMC Urol

View full text Add to dashboard Cite

Background and aims It has been reported that Helicobacter pylori (HP) infection was more prevalent in infertile populations. HP infection could lead to decreased sperm parameters, and treating the HP infection could improve the quality of sperm. However, studies investigating the relationship between infertility and HP infection are still limited, and more evidence is required. Therefore, we performed the present study to investigate the impact of HP infection on sperm quality in males and on ovarian reserve in females. Methods A total of 16,522 patients who visited the Second Hospital of Zhejiang University from January 2016 to June 2019 due to abdominal discomfort and underwent a 13/14C-urea breath HP test were included in this retrospective cross-sectional study. Among them, 565 had performed sperm analysis or ovarian reserve tests in the past three months and were involved for further analyses. Sperm parameters were examined with a computer-assisted sperm analysis system, and serum anti-Müllerian hormone (AMH) and sex hormones were tested with an electrochemiluminescence method. Results Among 363 patients who underwent the sperm test, 136 (37.47%) had HP infection. Among 202 patients who underwent the AMH test, 55 (27.23%) had HP infection. There was no difference in sperm concentration and motility between the HP+ and HP− groups (P > 0.05). Further subgroup analyses stratified into 5-year age groups confirmed that there was no significant difference in sperm parameters (P > 0.05). When pooled with previously published data, no significant difference in sperm concentration or motility was found (P > 0.05). Meanwhile, this study found that the serum AMH level was similar between the HP+ and HP− groups (P > 0.05). Further subgroup analyses confirmed that there was no significant difference in serum AMH level (P > 0.05). Conclusions There were no differences in sperm parameters and AMH levels based on history of HP infection among Chinese patients.

show abstract

“…The diagnostic accuracy was determined using samples from 213 dyspeptic patients, and UBT was the reference. The authors concluded that, in terms of accuracy, the latex immunoassays are as reliable as ELISAs but they have some advantages in terms of time and cost 33 …”

Section: Non‐invasive Testsmentioning

confidence: 99%

Review: Diagnosis ofHelicobacter pyloriinfection

2020

View full text Add to dashboard Cite

New endoscopic techniques, especially linked colour imaging (LCI) and blue laser imaging (BLI), were extensively evaluated this year either for the diagnosis of Helicobacter pylori gastritis or the detection of early gastric cancer (EGC). In the first case, the studies followed a similar protocol. Endoscopy was performed with the new imaging technique as well as white light imaging (WLI) on a group of patients for whom the H pylori status was established by various methods. Several images were obtained from different parts of the stomach and submitted blindly to a group of endoscopists (at least 4) for evaluation. The first aim was to determine the sensitivity and specificity of the new method vs WLI. LCI always performed better than WLI with sensitivity and specificity for LCI ranging from 83.8% to 85.4% and 79.5% to 99.5%, respectively (Table 1). The difficulty stemmed from the fact that there are different stages of H pylori gastritis, from active inflammation to atrophy and intestinal metaplasia, which exhibit very different aspects. Nevertheless in their study, Wang et al concluded that identification of H pylori gastritis by LCI was especially reliable in the corpus. 1 In another study by Jiang et al, using a score of 3.5 as the cutoff value, sensitivity and specificity were 83.8% and 99.5%, respectively, for the diagnosis of H pylori gastritis among 358 patients. 2 Ono et al 3

show abstract

Comparison of the detection of Helicobacter pylori infection by commercially available serological testing kits and the 13C-urea breath test

Cited by 13 publications

References 19 publications

What Is New in Helicobacter pylori Diagnosis. An Overview

What Is New in Helicobacter pylori Diagnosis. An Overview

Sperm parameters and anti-Müllerian hormone remain stable with Helicobacter pylori infection: a cross-sectional study

Review: Diagnosis ofHelicobacter pyloriinfection

Contact Info

Product

Resources

About