Comparison of the Cytotoxicity of Different Hydroperoxides to V79 Cells

Nakayama, Tsutomu; Hori, Kenzo; Terazawa, Kaoru; Kawakishi, Shunrô

doi:10.3109/10715769109088946

Cited by 19 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are many reports describing the cytotoxic effects of H202 On mammalian cells, including viability, DNA Iesions, mutation, chromosomal aberration, and morphological transformation (Gille & Joenje, 1991 ). We have already reported that various kinds of natural and synthetic polyphenols prevent cytotoxicity on cultured mammalian cells caused by H202 (Nakayama et a/., 1991(Nakayama et a/., , 1992a(Nakayama et a/., ,b, 1993Nakayama, 1994). Specifically, flavonoids such as quercetin and catechin prevented H202-induced cytotoxicity toward Chinese hamster lung V79 cells at concentrations that were not themselves cytotoxic (Nakayama et a/., 1993).…”

mentioning

confidence: 99%

Hydrogen Peroxide Formation during Catechin Oxidation Is Inhibited by Superoxide Dismutase.

Nakayama

Enoki

Hashimoto

1995

FSTI

View full text Add to dashboard Cite

We established a simple method to quantify hydrogen peroxide (H202) generated during the aerobic heating process of (+)-catechin solution. Using this method, we found that non-enzymatic H202 formation from catechin depended on pH, temperature, incubation time, and the presence of 02 in the solution. The formation of oxidized products of (+)-catechin, which was estimated by measuring the absorbance of the solution at 430 nm, also depended on these factors. The H202 formation was inhibited by various kinds of superoxide dismutase (SOD) with almost the same dose dependency. Although the oxidation of (+)-catechin was enhanced by superoxide (02-), neither catalase nor H202 had any effects on the oxidation. These results suggest that 02-, rather than H202, participated in the autoxidation of (+)-catechin, which was coupled with the H202 formation.

show abstract

mentioning

confidence: 99%

Hydrogen Peroxide Formation during Catechin Oxidation Is Inhibited by Superoxide Dismutase.

Nakayama

Enoki

Hashimoto

1995

FSTI

View full text Add to dashboard Cite

show abstract

“…It inhibits lipoxygenase activity (Kemal et al, 1987) and arachidonic acid release (Robinson et al, 1990). It suppresses cytotoxicity of hydroperoxides such as t-butylhydroperoxide (Nakayama et al, 1991) and hydrogen peroxide (Nakayama et al, 1992) to mammalian cells. Some antioxidants are known to prevent oxidative stress-induced toxicity.…”

Section: Resultsmentioning

confidence: 99%

Prooxidant activity and toxicity of nordihydroguaiaretic acid in clone-9 rat hepatocyte cultures

Sahu

Ruggles

O’Donnell

2006

Food and Chemical Toxicology

View full text Add to dashboard Cite

Nordihydroguaiaretic acid (NDGA) is a polyphenol. It is present at high concentrations in the leaves of the evergreen desert shrub, Larrea tridentate (Creosote bush), which has a long history of medicinal use traditionally by the native Americans and Mexicans. It is generally believed that the antioxidant properties of NDGA are responsible for the medicinal value of this desert shrub. The clone-9 rat hepatocyte cultures were used as an in vitro model to assess the hepatotoxic potential of NDGA and to determine whether it exhibits any prooxidant activity. The hepatocyte cultures were treated with NDGA for 2 h at 37 degrees C at concentrations of 0-100 microM. After the treatment period the cells, the culture supernatants and cell lysates were assayed for evaluation of prooxidant activity and toxicity of NDGA. Oxidative stress level and oxidative cell injury as measured by the peroxidation of membrane lipids and DNA double-strand breaks were used to index prooxidant activity. Cytotoxicity as measured by the leakage of the liver enzyme lactate dehydrogenase (LDH) into the culture medium, mitochondrial function and extent of cell proliferation were used as the endpoints of toxicity. Significant concentration-dependent differences were observed in these biomarkers over the concentration range examined demonstrating the prooxidant activity and toxicity of NDGA in clone-9 rat hepatocyte cultures.

show abstract

“…It is of interest that despite the noted antioxidant activity observed with RVS, this property did not interfere with the inhibition of cell proliferation observed for the RVS extract in both the HeLa and CT-26 tumor cell lines. Lipid oxidation products such as linoleic acid hydroperoxides are well known to exhibit cytotoxic effects in a number of cell lines (Nakayama et al, 1991), and protection of cytotoxicity induced by lipophilic hydroperoxides has been reported in endothelial cells with the antioxidant a -tocopherol (Kaneko et al, 1991). Alternatively, highly hydrophilic or lipophilic antioxidants are not effective at preventing cytotoxicity induced by lipid oxidation products (Nakayama et al, 1991).…”

Section: Discussionmentioning

confidence: 98%

“…Lipid oxidation products such as linoleic acid hydroperoxides are well known to exhibit cytotoxic effects in a number of cell lines (Nakayama et al, 1991), and protection of cytotoxicity induced by lipophilic hydroperoxides has been reported in endothelial cells with the antioxidant a -tocopherol (Kaneko et al, 1991). Alternatively, highly hydrophilic or lipophilic antioxidants are not effective at preventing cytotoxicity induced by lipid oxidation products (Nakayama et al, 1991). Further work is necessary to elucidate the mechanism(s) of action for RVS on cell cycle progression and the possibility that the active components may modulate cell cycle regulatory function.…”

Section: Discussionmentioning

confidence: 99%

ANTITUMORIGENIC AND CYTOTOXIC PROPERTIES OF AN ETHANOL EXTRACT DERIVED FROMRhus VernicifluaSTOKES (RVS)

Kitts¹,

Lim²

2001

Journal of Toxicology and Environmental Health, Part A

View full text Add to dashboard Cite

In this study, the antioxidant, cytotoxic, and antitumorigenic activities of a fractionated, ethanol extract derived from Rhus verniciflua Stokes (RVS), a plant indigenous to Korea, China, and Japan, were determined. Physicochemical analysis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) results indicated that the active component of a Sephadex G-150-fractionated RVS extract (PII fraction) was a copper-containing glycoprotein, possibly a plant laccase. Antioxidant activity of the fractionated RVS extract, observed in both aqueous and lipid in vitro oxidation reactions using 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical, site-specific Fenton-reaction deoxyribose, and a model lipid emulsion test system, indicated an affinity for protection against hydroxyl and peroxyl radicals. Cultured mouse brain neurons were protected against glucose oxidase-induced hydroxyl radical in the presence of the fractionated RVS extract (e.g., 58% protection at 4.9 microM and 95% protection with 22.7 microM RVS). RVS was further shown to protect against in vitro Fenton-reaction-induced single- and double-strand scission in supercoiled plasmid DNA. Further testing for bioactivity of the fractionated RVS extract was based on the affinity to inhibit cell proliferation in cultured HeLa and CT-26 tumor cells. The presence of RVS resulted in 70% cell death after 24 h of incubation in both cell lines at a minimum concentration of 2.48 microM RVS. Data demonstrate multiple bioactive chemopreventative properties of a Sephadex G-150-fractionated extract derived from RVS.

show abstract

Comparison of the Cytotoxicity of Different Hydroperoxides to V79 Cells

Cited by 19 publications

References 14 publications

Hydrogen Peroxide Formation during Catechin Oxidation Is Inhibited by Superoxide Dismutase.

Hydrogen Peroxide Formation during Catechin Oxidation Is Inhibited by Superoxide Dismutase.

Prooxidant activity and toxicity of nordihydroguaiaretic acid in clone-9 rat hepatocyte cultures

ANTITUMORIGENIC AND CYTOTOXIC PROPERTIES OF AN ETHANOL EXTRACT DERIVED FROMRhus VernicifluaSTOKES (RVS)

Contact Info

Product

Resources

About