Comparison of the antibacterial activity of honey from different provenance against bacteria usually isolated from skin wounds

Basualdo, Claudia; Sgroy, Verónica; Finola, Mónica S.; Marioli, Juan M.

doi:10.1016/j.vetmic.2007.04.039

Cited by 155 publications

(135 citation statements)

References 14 publications

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“…These authors also observed that S. aureus was the most sensitive bacteria to manuka and pasture honeys. Results of the inhibitory activity of raw honey against pathogens have been presented by Taormina et al (2001) and Basualdo et al (2007) which are similar to the results obtained in this work, that have been carried out in different experimental conditions.…”

Section: Antimicrobial Assayssupporting

confidence: 83%

“…Although the honey therapeutic action has been taken some attention by researchers, studies only have been done on screening the raw honey samples on antimicrobial activity (Taormina et al, 2001;Basualdo et al, 2007) and on antioxidant capacity (Rauha et al, 2000;Frankel et al, 1998). Others studies have shown that individual phenolic compounds have growth inhibition on a wide range of Gram-positive and Gram-negative bacteria (Davidson et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey

Estevinho

Pereira

Moreira

et al. 2008

Food and Chemical Toxicology

439

255

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Phenolic compounds of dark and clear honeys from Trás-os-Montes of Portugal were extracted with Amberlite XAD-2 and evaluated for their antioxidant and antimicrobial activities. The antioxidant effect was studied using the in vitro test capacity of scavenge the 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical and of reducing power of iron (III)/ferricyanide complex. The antimicrobial activity was screened using three Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, Staphylococcus lentus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli). The results obtained from the partial identification of honey phenolic compounds by high-performance liquid chromatography with a diode array detector showed that p-hydroxibenzoic acid, cinnamic acid, naringenin, pinocembrin and chrysin are the phenolic compounds present in most of the samples analyzed. Antioxidant potential was dependent of honey extract concentration and the results showed that dark honey phenolic compounds had higher activity than the obtained from clear honey. In the biological assays, results showed that S. aureus were the most sensitive microrganisms and B. subtilis, S. lentus, K. pneumoniae and E. coli were each moderately sensitive to the antimicrobial activity of honey extracts. Nevertheless, no antimicrobial activity was observed in the test with P. aeruginosa.

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Section: Antimicrobial Assayssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey

Estevinho

Pereira

Moreira

et al. 2008

Food and Chemical Toxicology

439

255

View full text Add to dashboard Cite

show abstract

“…The difficulty in Enterococcus faecalis treatment has been attributed to the lack of anti-infective strategies to eradicate its biofilm and to the frequent emergence of multidrug-resistant strains. Several studies have shown that various topical antimicrobial agents including honey and herbal extracts are ineffective for the eradication of Enterococcus faecalis biofilm (Basualdo et al, 2007;Nidadavolu et al, 2012;Hajská et al, 2014). The observations obtained in the present study suggest that, from a clinical point of view, infected wounds associated with Enterococcus faecalis biofilm could be unresponsive to topical honey treatment.…”

Section: Discussionsupporting

confidence: 44%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the antibiofilm activity of honey defensin-1 (Def-1) in its recombinant form. A modified Lubbock chronic wound biofilm formed by four bacterial species (Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas aeruginosa and Enterococcus faecalis) was used for evaluation of honey and recombinant bee-derived Def-1 antibiofilm efficacy. Recombinant Def-1 was prepared by heterologous expression in Escherichia coli. We showed that different types of honey (manuka and honeydew) were able to significantly reduce the cell viability of wound pathogens (Staphylococcus aureus, Streptococcus agalactiae and Pseudomonas aeruginosa) in mature polymicrobial biofilm. None of the tested honeys showed the ability to eradicate Enterococcus faecalis in biofilm. In addition, recombinant Def-1 successfully reduced the viability of Staphylococcus aureus and Pseudomonas aeruginosa cells within established polymicrobial biofilm after 24 and 48 h of treatment. Interestingly, recombinant Def-1 did not affect the viability of Streptococcus agalactiae cells within the biofilm, whereas both natural honeys significantly reduced the viable bacteria. Although Enterococcus faecalis was highly resistant to Def-1, Def-1 significantly affected the biofilm formation of Enterococcus faecalis and Streptococcus agalactiae after 24 h of treatment, most likely by inhibiting its extracellular polymeric substances production. In conclusion, our study revealed that honey and Def-1 are effective against established multispecies biofilm; however, Enterococcus faecalis grown in multispecies biofilm was resistant to both antimicrobials.

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“…as fungal strains. Results: The range of the diameter of zone of inhibition of various concentrations of tested honeys was (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23) mm) for Rhodotorula sp., while C. albicans showed clearly resistance towards all concentrations used. The MICs of tested honey concentrations against C. albicans and Rhodotorula sp.…”

Section: Honey Samplesmentioning

confidence: 99%

“…Most types of honey generate hydrogen peroxide when diluted because of the activation of the enzyme glucose oxidase, which oxidizes glucose to gluconic acid and hydrogen peroxide [17,18] . Hydrogen peroxide is the major contributor to the antimicrobial activity of honey, and the different concentrations of this compound in different honeys result in their varying antimicrobial effects [19][20][21][22] . The in vitro antifungal activity of honey was reported by Maria et al [23] , who observed that honey stops the growth of C. albicans, Candida krusei and Cryptococcus neoformans.…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of four honeys of different types from Algeria against pathogenic yeast: Candida albicans and Rhodotorula sp.

Mustafa¹,

Djebli

Saad³

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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Comparison of the antibacterial activity of honey from different provenance against bacteria usually isolated from skin wounds

Cited by 155 publications

References 14 publications

Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey

Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Antifungal activity of four honeys of different types from Algeria against pathogenic yeast: Candida albicans and Rhodotorula sp.

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