Comparison of Temporary and Continuous Immersion Systems for Micropropagation of Axillary Shoots of Chestnut and Willow

Vidal, Nieves; Correa, Begoña; Rial, E. J.; Regueira, María; Sánchez, C.; Cuenca, B.

doi:10.17660/actahortic.2015.1083.27

Cited by 7 publications

(10 citation statements)

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“…In a former study of our research group, we explored the propagation of Salix viminalis (willow) and chestnut in liquid medium, either with temporary or continuous immersion systems. The results of this preliminary study were recently reported (Vidal et al 2015), and highlighted the different performance of these two woody species in liquid medium. Although the possibility of culturing chestnut shoots by TIS was demonstrated for some genotypes (Vidal et al 2015), shoot quality and proliferation rates were low, and should be improved before applying this system to largescale propagation.…”

Section: Introductionsupporting

confidence: 64%

“…comm. ) and also in preliminary experiments in TIS (Vidal et al 2015). In clone 111, basal explants cultured in twin flasks (5 l) mainly yielded non-hyperhydric shoots that were similar to those cultured in SS medium and that were more than twice as long as nodal explants (Fig.…”

Section: Resultsmentioning

confidence: 77%

“…High levels of hyperhydricity were also observed by Troch et al (2010), which considered this disorder as a major obstacle to culture European chestnut shoots in liquid medium. Hyperhydric shoots have not been observed during the culture of other tree species in TIS, such as Crescentia cujete (Murch et al 2004) and willow (Vidal et al 2015). However, a certain level of hyperhydricity has been reported in many woody species cultured in TIS, such as apple (Chakrabarty et al 2003;Zhu et al 2005), eucalyptus (McAlister et al 2005), cloudberry (Rubus chamaemorus) and blueberry (Debnath 2007(Debnath , 2009), teak (Quiala et al 2012), walnut (Moreno et al 2012), and pistachio (Akdemir et al 2014).…”

Section: Resultsmentioning

confidence: 98%

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A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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A protocol for culturing chestnut axillary shoots by temporary immersion in liquid medium was developed. The influence of type of explant, support material, bioreactor, and immersion was investigated for five artificial hybrids and five natural hybrids of Asian and European chestnut selected for resistance to ink disease. The type of explant influenced shoot quality and proliferation rates, and basal explants with callus produced more and longer shoots than apical and nodal segments. Use of rockwool cubes as support material prevented hyperhydricity and allowed proliferation of explants in Murashige and Skoog medium with half-strength nitrates supplemented with 0.22 lM BA and 3 % sucrose, cultured both in plantform TM and RITA Ò vessels with three or six immersions per day and additional aeration of 1 min per hour in the case of plantform TM bioreactors. Basal explants cultured in plantform TM for 5 weeks produced long shoots suitable for rooting, whereas apical and nodal explants cultured in plantform TM or RITA Ò produced shorter shoots that were suitable for maintenance of stock. For most of the clones, similar or higher proliferation rates were observed when cultured in liquid medium than in semisolid medium, with the additional benefit of cost-reduction of the former system. Shoots developed in liquid medium were submitted to ex vitro root induction by dipping in indole-3-butyric acid, and acclimatized under greenhouse conditions. This is the first demonstration of the production of chestnut plantlets from shoots cultured in liquid medium, and the protocol presented here shows good potential for application in large-scale propagation.

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Section: Introductionsupporting

confidence: 64%

Section: Resultsmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 98%

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A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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“…One research limitation of this particular study is indeed the small number of papers that compared the efficacy of more than one bioreactor as cultivation system. Of a total of 20 articles only 2 compared more than one bioreactor system (Vidal et al, 2015a;Vidal et al, 2015b). Since the work of Harris and Mason (1983), several TISs have been developed and the interest in this culture technique has progressively increased and shown promising results when compared to the traditional micropropagation system in semi-solid medium.…”

Section: Discussionmentioning

confidence: 99%

“…Although eucalyptus was the most studied species, other species of great economic interest also appeared in the papers, such as apple, followed by chestnut and pistachio (Chakrabarty et al, 2003(Chakrabarty et al, , 2007Zhu et al, 2005;Troch et al, 2010;Akdemir et al, 2014;Tilkat et al, 2014;Vidal et al, 2015a).…”

Section: Speciesmentioning

confidence: 99%

Temporary Immersion System for Micropropagation of Tree Species: a Bibliographic and Systematic Review

Carvalho

Özüdoğru²,

Lambardi

et al. 2018

Not Bot Horti Agrobo

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This paper was characterized by a bibliometric and systematic review on the database ISI Web of Science, aiming to provide an update of the main points addressed regarding the Temporary Immersion Systems (TIS) for micropropagation of tree species. It was pointed out that the frequency and time of immersion were one of the main parameters studied in the papers and 35% these papers worked with eucalyptus species. The main problem reported in the papers was the hyperhydricity, but it was overcome via procedures such as: a) air injection into the system, b) increasing the immersion intervals and decreasing the immersion time and c) decreasing concentration of cytokinin. Most papers reported that TIS produced plants that were more successful in surviving the ex-vitro acclimation stage than those produced on semi-solid media or continuous immersion systems. Few studies compared different types of temporary immersion bioreactors on micropropagation of plants and within the established criteria, papers with TIS tree species represented only 15% of the total. This system has presented promising results for most of the tree species, and although some gaps have been identified and few are the works with tree species, this process has been taking an increasingly larger space in the propagation of plants.

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Micropropagation of axillary shoots of Salix viminalis using a temporary immersion system

Regueira

Rial

Blanco

et al. 2017

Trees

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Comparison of Temporary and Continuous Immersion Systems for Micropropagation of Axillary Shoots of Chestnut and Willow

Cited by 7 publications

References 0 publications

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Temporary Immersion System for Micropropagation of Tree Species: a Bibliographic and Systematic Review

Micropropagation of axillary shoots of Salix viminalis using a temporary immersion system

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