Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro

Kovács, Anita; Zsikó, Stella; Falusi, Fanni; Csányi, Erzsébet; Budai-Szűcs, Mária; Csóka, Ildikó; Berkó, Szilvia

doi:10.3390/pharmaceutics13122106

Cited by 10 publications

(1 citation statement)

References 26 publications

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“…Starting from the basis of microporous filter membrane and PDMS film studies, it was verified whether the lipid exchange effect is the main cause of drug permeation differences. Start-M membranes are commercially available artificial membranes that can replace the skin and have a structural composition that mimics skin lipids, which is not found in microporous membranes and PDMS membranes, so Start-M membranes were chosen as the diffusion medium for the experiments (Haq et al., 2018 ; Kovács et al., 2021 ), and the rest of the procedure was the same as in Section 2.1.3.…”

Section: Methodsmentioning

confidence: 99%

Study of the permeation-promoting effect and mechanism of solid microneedles on different properties of drugs

Peng

Song

et al. 2023

Drug Delivery

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In transdermal drug delivery systems, the physicochemical properties of the drug affect its percutaneous permeability. However, whether the physicochemical properties of drugs change their transdermal permeability in the presence of pores in the presence of solid microneedles (MNs) has been less studied in this area. In this project, cinnamaldehyde, curcumin, ferulic acid and geniposide were selected as model drugs for the study of their transdermal permeability under the action of MNs, and a combination of classical experiments and visualization means such as scanning electron microscopy and laser confocal was used to investigate the permeation-promoting mechanism of MNs. The results showed that the MNs had significant permeation-promoting effects on different properties of drugs, with the permeation-promoting effects on cinnamaldehyde, curcumin, ferulic acid and geniposide being 6.36, 17.43, 29.54 and 8.91 times, respectively, and the permeation-promoting effects were more pronounced for lipid-soluble and amphiphilic drugs. Using scanning electron microscopy, transmission electron microscopy and other means to confirm that MNs can promote the penetration by acting on the skin to produce pores, and their effect on skin structure is greater than that of drugs. In addition, the existence of pores increases the amount of drug transdermal, which may enhance the diffusion of drug on the skin, and has no effect on lipid exchange and transdermal route. Through the research, it has been found that MNs is equivalent to direct peeling of the stratum corneum (SC), but it is simpler and safer for the patient.

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Section: Methodsmentioning

confidence: 99%

Study of the permeation-promoting effect and mechanism of solid microneedles on different properties of drugs

Peng

Song

et al. 2023

Drug Delivery

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3D Bioprinted Human Skin Model Recapitulating Native‐Like Tissue Maturation and Immunocompetence as an Advanced Platform for Skin Sensitization Assessment

Bhar,

Das,

Manikumar

et al. 2024

Adv Healthcare Materials

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Physiologically‐relevant in vitro skin models hold utmost importance for efficacy assessments of pharmaceutical and cosmeceutical formulations, offering valuable alternatives to animal testing. Here, we present an advanced immunocompetent three‐dimensional (3D) bioprinted human skin model to assess skin sensitization. Initially, a photopolymerizable bioink is formulated using silk fibroin methacrylate, gelatin methacrylate, and photoactivated human platelet releasate. The developed bioink shows desirable physicochemical and rheological attributes for microextrusion bioprinting. The tunable physical and mechanical properties of bioink were modulated through variable photocuring time, aiding control over mechanoresponsive cellular behaviour. Thereafter, the bioink is utilized to 3D bioprint “sandwich type” skin construct where an artificial basement membrane supports a biomimetic epidermal layer on one side and a printed pre‐vascularized dermal layer on the other side within a transwell system. The printed construct is further cultured in air‐liquid interface for maturation. Immunofluorescence staining demonstrated a differentiated keratinocyte layer and dermal extracellular matrix (ECM)‐remodelling by fibroblasts and endothelial cell innervation. The biochemical estimations and gene‐expression analysis validate the maturation of the printed model. Incorporation of macrophages further enhances physiological relevance of the model. Our model effectively classifies skin irritative and non‐irritative substances, thus establishing itself as a suitable pre‐clinical screening platform for sensitization tests.This article is protected by copyright. All rights reserved

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Characterization of percutaneous absorption of calcium, magnesium, and potentially toxic elements in two tailored sulfurous therapeutic peloids: a comprehensive in vitro pilot study

Bastos,

Rocha,

Patinha

et al. 2024

Int J Biometeorol

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Pelotherapy treatments in thermal spas, which utilize peloids composed of clay minerals mixed with saltwater or mineral-medicinal water, can have various effects on spa users, ranging from therapeutic to potential adverse reactions. Despite the widespread use of peloids, comprehensive information on the penetration and permeation of essential and potentially toxic elements into deeper layers of the skin during pelotherapy is limited. Understanding the concentrations of these elements is crucial for evaluating therapeutic benefits and ensuring safety. This study investigates the in vitro availability and absorption of calcium, magnesium, and potentially toxic elements in two peloids, considering their formulation matrix. To replicate the pelotherapy methodology, an in vitro permeation experiment was conducted using a vertical diffusion chamber (Franz cells) and a biological system with human skin membranes from five Caucasian women, age range between 25 and 51 years. The experiment involved heating the peloids to 45℃. The results emphasize the possible transport properties of chemical elements in peloids, providing valuable information related to potential therapeutic efficacy and safety considerations. Despite no apparent differences between peloids’ chemical composition, the method identified permeation variations among chemical elements. The methodology employed in this study adheres to the guidelines outlined by OECD for analyzing skin absorption through an in vitro approach. Furthermore, it aligns with the associated OECD guidance document for conducting skin absorption studies. The replicability of this methodology not only facilitates the analysis of peloids pre-formulation but also provides a robust means to evaluate the effectiveness of therapeutic elements during topical administration, particularly those with potential toxicity concerns.

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Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro

Cited by 10 publications

References 26 publications

Study of the permeation-promoting effect and mechanism of solid microneedles on different properties of drugs

Study of the permeation-promoting effect and mechanism of solid microneedles on different properties of drugs

3D Bioprinted Human Skin Model Recapitulating Native‐Like Tissue Maturation and Immunocompetence as an Advanced Platform for Skin Sensitization Assessment

Characterization of percutaneous absorption of calcium, magnesium, and potentially toxic elements in two tailored sulfurous therapeutic peloids: a comprehensive in vitro pilot study

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